The present invention, in which RPE cells are suspended in a medium pharmaceutically acceptable as an ocular irrigating/washing solution and containing a poloxamer, achieves improvement of the post-thawing survival rate of cryopreserved RPE cells, improvement of the photoreceptor cell protection effect by RPE cell transplanted immediately after thawing, and prevention of loss of RPE cells in various steps from thawing to transplantation.

The present invention relates to a kit for preparing a customizable flesh simulating silicone gel or a flesh simulating silicone foam in particular for use in medical devices and a process for preparing said customizable flesh simulating silicone gel or silicone foam, in particular by using a 3D-printer.

The present invention relates to a kit for preparing a customizable flesh simulating silicone gel or a flesh simulating silicone foam in particular for use in medical devices and a process for preparing said customizable flesh simulating silicone gel or silicone foam, in particular by using a 3D-printer.

Embodiments of the present invention relate to methods for preparing high optical density solutions of nanoparticle, such as nanoplates, silver nanoplates or silver platelet nanoparticles, and to the solutions and substrates prepared by the methods. The process can include the addition of stabilizing agents (e.g., chemical or biological agents bound or otherwise linked to the nanoparticle surface) that stabilize the nanoparticle before, during, and/or after concentration, thereby allowing for the production of a stable, high optical density solution of silver nanoplates. The process can also include increasing the concentration of silver nanoplates within the solution, and thus increasing the solution optical density.

Embodiments of the present invention relate to methods for preparing high optical density solutions of nanoparticle, such as nanoplates, silver nanoplates or silver platelet nanoparticles, and to the solutions and substrates prepared by the methods. The process can include the addition of stabilizing agents (e.g., chemical or biological agents bound or otherwise linked to the nanoparticle surface) that stabilize the nanoparticle before, during, and/or after concentration, thereby allowing for the production of a stable, high optical density solution of silver nanoplates. The process can also include increasing the concentration of silver nanoplates within the solution, and thus increasing the solution optical density.

An object of the present invention is to provide a novel method which enables convenient preparation of cells exhibiting functions close to that of intestinal epithelial cells of living bodies, and use of the method. The differentiation of induced pluripotent stem cells into intestinal epithelial cells is induced by step of differentiating induced pluripotent stem cells into endoderm-like cells; step of differentiating the endoderm-like cells obtained in step into intestinal stem cell-like cells; and step of differentiating the intestinal stem cell-like cells obtained in step into intestinal epithelial cell-like cells, wherein step includes culture in the presence of a MEK1 inhibitor, a DNA methyltransferase inhibitor, a TGF-β receptor inhibitor, and EGF and under the condition that cAMP is supplied to the cells.

An object of the present invention is to provide a novel method which enables convenient preparation of cells exhibiting functions close to that of intestinal epithelial cells of living bodies, and use of the method. The differentiation of induced pluripotent stem cells into intestinal epithelial cells is induced by step of differentiating induced pluripotent stem cells into endoderm-like cells; step of differentiating the endoderm-like cells obtained in step into intestinal stem cell-like cells; and step of differentiating the intestinal stem cell-like cells obtained in step into intestinal epithelial cell-like cells, wherein step includes culture in the presence of a MEK1 inhibitor, a DNA methyltransferase inhibitor, a TGF-β receptor inhibitor, and EGF and under the condition that cAMP is supplied to the cells.

A method of acoustophoretic printing comprises generating an acoustic field at a first end of an acoustic chamber fully or partially enclosed by sound-reflecting walls. The acoustic field interacts with the sound-reflecting walls and travels through the acoustic chamber. The acoustic field is enhanced in a chamber outlet at a second end of the acoustic chamber. An ink is delivered into a nozzle positioned within the acoustic chamber. The nozzle has a nozzle opening projecting into the chamber outlet. The ink travels through the nozzle and is exposed to the enhanced acoustic field at the nozzle opening, and a predetermined volume of the ink is ejected from the nozzle opening and out of the acoustic chamber.

A method for constructing a three-dimensional multi-scale surface to obtain controlled and improved physical and chemical configurations to promote the integration of orthopedic and/or dental implants, to human and/or animal tissues, in different shapes and geometries in a versatile manner, and can be applied to all types of metals, metal alloys and/or ceramic compounds. This method includes the modification at the macroscopic level of the roughness, with an objective of promoting the mechanical interlocking of the implant, followed by the modification of the surface for the formation of microtopography, then the microtopography is changed to obtain a nanotopography with characteristics that optimize cellular metabolic responses related to attraction, adhesion, spreading, proliferation and cell growth, in addition to phenotypic and genotypic inductions in undifferentiated cells and in osteoblast lineage, responsible for mineralization and bone neoformation. As a result, the interface between implant and bone is improved.

The present invention relates to a raw material for a bio-3D printing support and, more specifically, to a novel type bio-3D printing support material for tissue engineering, a method for manufacturing a three-dimensional support by using the same, and a 3D-printing three-dimensional support manufactured thereby, the raw material: being non-toxic and implementing excellent biocompatibility and cell adhesion since a raw material for a tissue engineering support (scaffold) produced by bio-3D printing technology, a specific fatty acid and a fatty alcohol (phase change material) derived from a natural source having a low melting point and a low molecular weight are used; and, in particular, allowing a phase change to easily occur at a temperature similar to body temperature such that a process is simplified and cells or growth factors can be mixed.