The invention provides compositions and methods for making and using microvascular stamps for stimulation and spatial organization of neovessels in tissue.

The present invention is to provide a polymer scaffold for a prosthesis includes two outer sheets formed into a certain size and shape and made of a biodegradable synthetic polymer material, and an inner sheet disposed between the two outer sheets and made of a biodegradable natural polymer material, wherein the two outer sheets are formed in the form of a single sealed pouch by joining the edges of the two outer sheets together, and the outer sheets are formed by cutting a portion of a synthetic polymer matrix made of the biodegradable synthetic polymer material and having a larger area and thickness than the outer sheets, and the inner sheet is formed by cutting a portion of a natural polymer matrix made of the biodegradable natural polymer material and having a larger area and thickness than the inner sheet.

Tissue fillers derived from decellularized tissues are provided. The tissue fillers can include acellular tissue matrices that have reduced inflammatory responses when implanted in a body. Also provided are methods of making and therapeutic uses for the tissue fillers.

In one embodiment, the present invention provides a composition, wherein the composition is a porous scaffold, wherein the pores of the scaffold are from 1 to 500 microns, the composition comprising: a) a cross-linkable protein selected from the group consisting of collagen and gelatin; b) a cross-linker which induces cross-linking of the cross-linkable protein; and c) a liquid.

The proposed invention relates to the medical biotechnologies, to the tissue-derived approaches in regenerative medicine in particular. The method proposes, in addition to conservative treatment methods, substitution therapy for skin damage with the aid of polymer matrices, which are similar to the histotypically of the organism tissues with biologically active agents such as cellular derivatives (collagens and laminins) that contribute to the structural function of the damaged area. A biodegradable wound-healing composite material based on a combination of a polymer substrate and a product of the synthesis of human epithelial cells, devoid of the cell component, is characterized by a relative simplicity of manufacture, the long duration of the storage and can make possible to avoid skin grafting, for example in cases of deep and extensive burns, trophic ulcers, etc.

Methods of administering biological material to a subject are provided. Methods of culturing biological material are also provided. The methods include combining biological material with a thermally stable preparation having a purified amphiphilic peptide in an aqueous biocompatible solution. The peptide has a folding group having a plurality of charged amino acid residues and hydrophobic amino acid residues arranged in a substantially alternative pattern and a turn sequence. The peptide is configured to self-assemble into a hydrogel. The methods include combining the preparation with a buffer having an effective amount of an ionic salt and a biological buffering agent to form the hydrogel. The methods include administering the hydrogel and the biological material to a subject. Methods of biofabricating a hydrogel having biological material are also provided. Methods of facilitating cell therapy are also provided.

Provided herein is a method of preparing a crosslinked granular hydrogel, the method includes mixing a hydrogel microparticle having a first crosslinking group, a polymeric fiber having a second crosslinking group, an initiator, and a crosslinker to form a precursor composition. The method also includes applying focused ultrasound (FUS) to the precursor composition, whereby each of the first crosslinking group and the second crosslinking group reacts with the crosslinker, thereby the hydrogel microparticle and the polymeric fiber are crosslinked to form the granular hydrogel.

A multi-layer collagen-based membrane that includes a bioresorbable mesh embedded between a first decellularized natural collagen-based membrane and a second decellularized natural collagen-based membrane. The bioresorbable mesh can be formed of a synthetic polymer or demineralized laminar bone. Also provided are two methods for manufacturing a multi-layer collagen-based membrane with or without an embedded bioresorbable mesh.

Provided herein are constructs of micro-aggregate multicellular, minimally polarized grafts containing Leucine-rich repeat-containing G-protein coupled Receptor (LGR) expressing cells for wound therapy applications, tissue engineering, cell therapy applications, regenerative medicine applications, medical/therapeutic applications, tissue healing applications, immune therapy applications, and tissue transplant therapy applications which preferably are associated with a delivery vector/substrate/support/scaffold for direct application.

A multi-layer collagen-based membrane that includes a bioresorbable mesh embedded between a first decellularized natural collagen-based membrane and a second decellularized natural collagen-based membrane. The bioresorbable mesh can be formed of a synthetic polymer or demineralized laminar bone. Also provided are two methods for manufacturing a multi-layer collagen-based membrane with or without an embedded bioresorbable mesh.