The present invention provides a method for promoting the growth and preservation quality of Agaricus bisporus, and belongs to the technical field of cultivation and preservation of edible fungi. In the present invention, the methyl jasmonate solution is sprayed onto the surface of Agaricus bisporus at a concentration of 10-200 mol/L when the Agaricus bisporus grows to the mushroom bud stage, which can accelerate the growth and development of the Agaricus bisporus, improve the post-harvest preservation quality of the Agaricus bisporus, and prolong the shelf life thereof, thereby improving the yield and commercial value of the Agaricus bisporus. The present invention provides a new method for increase in both production and income and post-harvest preservation of Agaricus bisporus, can be applied to large-scale industrial production of Agaricus bisporus, and thus has important economic benefits and application promotion value.

The invention is a method for enhanced growth of mycelium in culture including the key steps of seed blend choice from among corn, rice, quinoa, chia, canihua, cumin and flax seed, germinating the seed blend prior to inoculating the mycelium starter, and conducting the mushroom cultivation with ORP enhanced water and particular transmissions of sound and particularly colored light. Mushrooms grown by such a method not only mature in 13-15 days rather than 21 days, but have an enhanced nutritional and medicinal profile along with absence of unwanted organism contaminants whose unwanted overgrowth occurs during days 16-21 (or days 16-28) of typical mushroom culture.

The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.

A mushroom culture of Agaricus bisporus, comprising at least one set of chromosomes as a culture of line J14756-s3, includes a representative culture of the line, which has been deposited under NRRL Accession No. 67317. A method of producing a hybrid mushroom culture of Agaricus bisporus comprising: mating a culture comprising at least one set of chromosomes as a culture of line J14756-s3 with a second homokaryotic line culture. Additionally, mushrooms, parts of the culture and products incorporating the culture of the invention are provided.

The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material.

The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material.

The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is a made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material. In another embodiment, the fungus is allowed to grow as a fruiting body out of the substrate and within an enclosure to completely fill the enclosure to form a self-supporting structure.

The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is a made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material. In another embodiment, the fungus is allowed to grow as a fruiting body out of the substrate and within an enclosure to completely fill the enclosure to form a self-supporting structure.

The present invention is related to an active substance of Hericium erinaceus having a pain-relieving effect, and a pharmaceutical composition including the active substance. The active substance is prepared using the following steps: (a) inoculating a mycelium of H. erinaceus on an agar plate and incubating at 15-32 C. for 8-16 days; (b) inoculating the incubated H. erinaceus mycelia from step (a) into a medium in a flask and incubating at 20-30 C. and pH 4.5-6.5 for 3-5 days; (c) inoculating the incubated H. erinaceus mycelia from step (b) into a medium in a fermentation tank and incubating at 24-32 C. and pH 4.5-5.5 for 8-16 days to obtain a fermented medium of the H. erinaceus mycelia; and (d) desiccating the fermented medium of the H. erinaceus mycelia from step (c) to obtain the powder of the H. erinaceus mycelia, which is further purified and isolated to obtain a novel compound of H. erinaceus.

The method grows a mycelial mass over a three-dimensional lattice such that a dense network of oriented hyphae is formed on the lattice. Growth along the lattice results in mycelium composite with highly organized hyphae strands and allows the design and production of composites with greater strength in chosen directions due to the organized nature of the supporting mycelia structure.