The present disclosure provides guided microbial remodeling (GMR) methods for the rational improvement of plant-associated microbes to perform plant-beneficial functions. The GMR methods described herein allow for non-intergeneric genetic optimization of key regulatory networks within the microbes, which improve plant-beneficial functions over wild-type microbes but don't have the risks associated with transgenic approaches (e.g., unpredictable gene function, public and regulatory concerns, etc.). The present disclosure also provides remodeled microbes and compositions thereof. The utilization of remodeled microbes and compositions thereof will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived fertilizers.

A method for remediating developmentally delayed plants within a field of crops using at least one work vehicle during a field operation. The method comprises identifying delayed plants within the field with a sensor on the work vehicle and generating, with a processor, location data associated with the location of the delayed plant with the field. Upon arriving at the location of the delayed plant with the work vehicle, the delayed plant is remediated.

A method for remediating developmentally delayed plants within a field of crops using at least one work vehicle during a field operation. The method comprises identifying delayed plants within the field with a sensor on the work vehicle and generating, with a processor, location data associated with the location of the delayed plant with the field. Upon arriving at the location of the delayed plant with the work vehicle, the delayed plant is remediated.

One aspect of the disclosure is directed to a method for activation/enhancement of cell growth of a plant. The method also stimulates the production of pharmaceutically active metabolites, including alkaloids, in plant cell cultures. The method includes providing a nano-sized material contained agent, and treating the plant with the nano-sized material contained agent to allow sufficient interaction of cells of the plant with the nano-sized material so as to activate/enhance the cell growth of the plant or to stimulate the production of pharmaceutically active metabolites.

One aspect of the disclosure is directed to a method for activation/enhancement of cell growth of a plant. The method also stimulates the production of pharmaceutically active metabolites, including alkaloids, in plant cell cultures. The method includes providing a nano-sized material contained agent, and treating the plant with the nano-sized material contained agent to allow sufficient interaction of cells of the plant with the nano-sized material so as to activate/enhance the cell growth of the plant or to stimulate the production of pharmaceutically active metabolites.

This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte.

This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte.

The disclosure is generally directed to methods for screening, identifying, and producing microorganisms capable of imparting beneficial properties to plants. In some aspects, improved plant-associated soil microorganisms are generated by experimental evolution using a plant root exudate or root exudate compound.

The disclosure is generally directed to methods for screening, identifying, and producing microorganisms capable of imparting beneficial properties to plants. In some aspects, improved plant-associated soil microorganisms are generated by experimental evolution using a plant root exudate or root exudate compound.

Described herein is the evaluation of the antifungal properties of bacterial strain KGS-3 against Fusarium Head Blight (FHB)) white mold, blackleg and a number of potato fungal diseases and the plant growth effect attained. KGS-3 is a novel strain of Paenibacillus polymyxa that can suppress bacterial and fungal plant diseases. KGS-3 is predicted to produce antifungal metabolites polymyxin, fusaricidin, and paenilarvin and has been demonstrated to produce cylindrol B. KGS-3 is a plant growth promoting bacteria demonstrated to increase protein content of plants and/or plant products.