The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.

Methods of in vitro clonal propagation, regeneration and transformation in Cannabis are provided. Also provided is the use of such methods in improvements of cannabis cultivars such as via breeding.

Provided are a composition for editing a Cannabis gene, a kit for editing a Cannabis gene, a kit for inhibiting Cannabis DNA expression and targeting the DNA, and a method of editing Cannabis DNA, each using a gene editing protein; a method of preparing an edited Cannabis gene including a step of forming calli; a gRNA for editing a Cannabis gene; and a use of the gRNA of the present invention and a use of both the gene editing protein and the gRNA for editing a Cannabis gene.

The present disclosure describes a method of culturing and harvesting plant shoot tips. The method includes providing a sterile vessel configured to hold at least one plant with one or more root masses and a first set of shoot tips, cutting across a base of the shoot tips with the one or more root masses held in the vessel to cut a first plurality of cut shoot tips at a first time; then growing a second set of shoot tips from the one or more root masses in the vessel; and then cutting across the one or more root masses held in the vessel to cut a second plurality of cut shoot tips. Plant tissue culture devices are also described.

The present description relates to a plant cutting device for cutting or cloning a plant, and housing the plant for growth. The plant cutting device generally comprises two compartments that are operatively connected together and displaceable from an open position to a closed position defining an enclosure, a cutter mounted within at least one of the compartments and configured to cut a plant part when the first and second compartments are displaced to the closed position, a cut end of the plant part being accommodated within the enclosure; and a growth medium disposed in the first compartment and/or the second compartment for contacting the cut end of the plant part allowing for the growth of the plant.

The invention provides compositions and methods for the breeding, production, processing and use of specialty cannabis.

Methods of propagating and growing poinsettias in pots have allowed poinsettias to become the highest selling potted flowering plant. The present application relates generally to the field of plant propagation. In particular, a method of producing a pre-pinched, callused poinsettia cutting is provided. Also provided is a method to produce a poinsettia with improved growth habit. The invention also provides a pre-pinched, callused poinsettia cutting.

The invention provides seeds and plants of cucumber hybrid SVCS0025 and cucumber inbred line ASL-M3092027M0. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of cucumber hybrid SVCS0025 and cucumber inbred line ASL-M3092027M0 and to methods for producing a cucumber plant produced by crossing such plants with themselves or with another plant, such as a cucumber plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of cucumber hybrid SVCS0025 and cucumber inbred line ASL-M3092027M0 comprising introduced beneficial or desirable traits.

The present invention provides a method for improving a one-time seedling rate of microspore embryoids of Brassica campestris ssp. Chinensis Makino, including the steps of: spraying 6-BA onto a flower bud of a plant; sterilizing the flower bud with alcohol and HgCl.sub.2; releasing microspores, filtering and centrifuging to obtain purified microspores; diluting the microspores with a NLN medium, subpackaging into culture dishes, and adding phytic acid; finally, subjecting to heat shock treatment and transferring to culture in the dark until embryoids appear, and then conducting shaking culture; transferring the cultured embryos in a cotyledon stage onto a MS medium for differentiation culture, wherein the culture conditions are: 4° C., 14 h of illumination by blue-red compound light/day, and 14 days of culture; and then continuing to culture under the condition of 25° C. and 14 h of illumination by blue-red compound light/day until seedlings.

The present invention provides a method for improving a one-time seedling rate of microspore embryoids of Brassica campestris ssp. Chinensis Makino, including the steps of: spraying 6-BA onto a flower bud of a plant; sterilizing the flower bud with alcohol and HgCl.sub.2; releasing microspores, filtering and centrifuging to obtain purified microspores; diluting the microspores with a NLN medium, subpackaging into culture dishes, and adding phytic acid; finally, subjecting to heat shock treatment and transferring to culture in the dark until embryoids appear, and then conducting shaking culture; transferring the cultured embryos in a cotyledon stage onto a MS medium for differentiation culture, wherein the culture conditions are: 4° C., 14 h of illumination by blue-red compound light/day, and 14 days of culture; and then continuing to culture under the condition of 25° C. and 14 h of illumination by blue-red compound light/day until seedlings.