A salt water supply unit includes a salt water plate that divides the interior of a salt water tank into a salt container and a salt water reservoir, and a salt water well that stands and penetrates the salt water plate. The salt water well accommodates a salt water valve device and a concentration detector. The salt water valve device includes a valve box having a valve hole that allows makeup water or salt water to flow therethrough, a float rod penetrating the valve hole, a valve element coupled to a first end of the float rod, and a water level detecting float coupled to a second end of the float rod. The concentration detector includes a switch that is incorporated in a stem holding a concentration detecting float. The switch outputs different detection signals in accordance with the position of the concentration detecting float.

A salt water supply unit includes a salt water plate that divides the interior of a salt water tank into a salt container and a salt water reservoir, and a salt water well that stands and penetrates the salt water plate. The salt water well accommodates a salt water valve device and a concentration detector. The salt water valve device includes a valve box having a valve hole that allows makeup water or salt water to flow therethrough, a float rod penetrating the valve hole, a valve element coupled to a first end of the float rod, and a water level detecting float coupled to a second end of the float rod. The concentration detector includes a switch that is incorporated in a stem holding a concentration detecting float. The switch outputs different detection signals in accordance with the position of the concentration detecting float.

The present disclosure relates to methods of protein purification by attaching an intein-C fragment to a target protein, passing a sample containing the intein-C tagged protein over a chromatographic resin carrying an intein-N fragment so as to create an intein-N intein-C complex, releasing the target protein from the intein-C fragment, and regenerating the column under conditions that disrupt the intein-N intein-C complex while preserving column functionality for multiple reuses.

The present disclosure relates to methods of protein purification by attaching an intein-C fragment to a target protein, passing a sample containing the intein-C tagged protein over a chromatographic resin carrying an intein-N fragment so as to create an intein-N intein-C complex, releasing the target protein from the intein-C fragment, and regenerating the column under conditions that disrupt the intein-N intein-C complex while preserving column functionality for multiple reuses.

The present disclosure relates to methods of protein purification by attaching an intein-C fragment to a target protein, passing a sample containing the intein-C tagged protein over a chromatographic resin carrying an intein-N fragment so as to create an intein-N intein-C complex, releasing the target protein from the intein-C fragment, and regenerating the column under conditions that disrupt the intein-N intein-C complex while preserving column functionality for multiple reuses.

The present disclosure relates to methods of protein purification by attaching an intein-C fragment to a target protein, passing a sample containing the intein-C tagged protein over a chromatographic resin carrying an intein-N fragment so as to create an intein-N intein-C complex, releasing the target protein from the intein-C fragment, and regenerating the column under conditions that disrupt the intein-N intein-C complex while preserving column functionality for multiple reuses.