Provided herein are devices, systems, and methods for particle sorting, including cell sorting, using microfluidics cartridges and microchips and the manufacture of the microfluidics cartridges and microchips by high-throughput approaches. Such methods, devices, and systems can be used to identify, sort, and collect a subset of particles or a single particle from a sample. The capability to manufacture such microfluidic tools in high volume may lower production costs and allow for the microfluidic tools to be used as consumables.

A device for performing an assay comprises a tube, a cap, an insert, and a reaction container. The tube includes a lateral flow strip disposed therein. The cap is coupled to the tube and includes a hollow interior defined at least partially therethrough. The insert is configured to be at least partially received within the hollow interior of the cap. The reaction container includes a cavity configured to store one or more fluids therein, and is rotatably coupled to the cap such that rotation of the cap relative to the reaction container causes (i) mixing of the one or more fluids and (ii) at least a portion of the mixed fluids to be delivered from the reaction container to the lateral flow strip via the insert.

A microfluidic sensing assembly may include a first structure supporting a sensor array, a second structure joined to the first structure and forming a microfluidic passage and a flat lens to focus light, following reflection of the light back and forth across the microfluidic passage, from the microfluidic passage onto the sensor array.

An assembly for use in a medical diagnostic device and system for analysis of one or more samples is disclosed. In one aspect of the invention, the assembly includes at least one extendable sample tray configured to hold the one or more samples. Additionally, the assembly includes at lease one holding unit coupled to the at least one extendable sample tray, wherein the holding unit is configured to hold a calibration marker. Furthermore, the extendable sample tray and the holding unit are arranged in the same plane and when the extendable sample tray is extended, the at least one holding unit is brought in a field of view of an image capturing unit.

The virus test device encompasses a pseudo-receptor film having pseudo-receptors mimicking a structure of a host-cell receptor, which binds specifically to a target virus, a virus introducing-tube for sucking down an air-under-test (AUT) containing the target viruses, to compress the AUT into a high-speed air-flow of aerosols-under-test, concentrating the target viruses contained in the AUT, and to eject the high-speed air-flow to the pseudo-receptor film, a signal conditioner for converting physical signals, which represent alterations of physical states of the pseudo-receptor film ascribable to specific bindings of the pseudo-receptors with the target viruses, to electric signals.

Verification plates for a bacterial endotoxin reader are provided, namely a temperature verification plate (TVP) and optical verification plate (OVP). The TVP has a body configured to be placed on a spindle of said reader and rotated by said spindle. The body has a temperature verification circuit with a temperature sensor and a temperature indicator. The temperature sensor is configured to measure a temperature of the body rotated by the spindle of the reader. The temperature indicator optically represents a value of the temperature measured by the temperature sensor. The temperature indicator is readable by an optical bench of the reader. The OVP has a body with a plurality of apertures located along a periphery that line up with an optical bench of the reader. Light produced by a light source of the reader can pass through the aperture and an intensity measured by a photodetector of the reader.

A micro total analysis system, operating method and manufacturing method thereof are provided. The micro total analysis system includes at least one micro total analysis unit each including: microfluidic device including first electrode and dielectric layer connected to each other, where the dielectric layer drives to-be-measured droplet to move based on voltage of the first electrode; and acoustic wave detection device including second electrode connected to the dielectric layer, where the dielectric layer is also used as transducer of the acoustic wave detection device, and configured to generate acoustic wave toward the droplet based on voltage of the second electrode, and generate a detection result corresponding to the droplet based on received acoustic wave. The micro total analysis system, the operating method and the manufacturing method thereof enables the microfluidic device and the acoustic wave detection device to be integrated in the same chip.

The present disclosure describes a throughput-scalable image sensing system for analyzing biological or chemical samples is provided. The system includes a plurality of image sensors configured to detect at least a portion of light emitted as a result of analyzing the biological or chemical samples. The plurality of image sensors is arranged on a plurality of wafer-level packaged semiconductor dies of a single semiconductor wafer. Each image sensor of the plurality of image sensors is disposed on a separate packaged semiconductor die of the plurality of packaged semiconductor dies. Neighboring packaged semiconductor dies are separated by a dicing street; and the plurality of packaged semiconductor dies and a plurality of dicing streets are arranged such that the plurality of packaged semiconductor dies can be diced from the single semiconductor wafer as a group.

Provided is a method of transferring a material into cells, comprising the steps of: forming droplets consisting of a material to be transferred and cells; and a step of subjecting the formed droplets to deformation, thereby transferring the material to be transferred, into the cells.

A digital microfluidics (DMF) device including an FET-biosensor (FETB) and method of field-effect sensing is closed. In some embodiments, the DMF device may include one or more FETBs integrated into the top substrate, the bottom substrate, or both the top and bottom substrates of the DMF device. In some embodiments, the DMF device may include one or more “drop-in” style FETBs in the top substrate, the bottom substrate, or both the top and bottom substrates of the DMF device. In some embodiments, the DMF device, FETB, and method of field-effect sensing provide active-matrix control integrated into an active-matrix DMF device. Further, a microfluidics system for and method of using the DMF device including at least one FETB is provided.