A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.

A method for processing a flowable product using a centrifuge includes pretreating the flowable product so that particles in the flowable product are increasingly attracted by electrically charged components, feeding the flowable product into the disc stack, generating an electric charge on the discs of the disc stack, and separating the charged particles from the flowable product within the disc stack under the influence of electrostatic attractive forces and a centrifugal field.

A method for processing a flowable product using a centrifuge includes pretreating the flowable product so that particles in the flowable product are increasingly attracted by electrically charged components, feeding the flowable product into the disc stack, generating an electric charge on the discs of the disc stack, and separating the charged particles from the flowable product within the disc stack under the influence of electrostatic attractive forces and a centrifugal field.

The disclosure relates to a dielectrophoretic tweezer, and associated methods of fabrication and use. The tweezer comprises a first end and a second end, in which the first end has a lateral dimension of less than 10 microns; a structure, extending in a longitudinal direction between the first and second ends, comprising an electrically insulating barrier defining a first chamber and a second chamber within the structure, in which the first and second chambers are insulated from each other by the electrically insulating barrier; a first electrode in the first chamber at the first end; and a second electrode in the second chamber at the first end, in which a width of the electrically insulating barrier separating the first electrode from the second electrode is 50 nm or less.

The present invention an apparatus and method of injecting a liquid borne sample into a field flow fractionator and a method of forming a top plate and spacer. In an embodiment, the field flow fractionation unit includes a top plate including a sample injection inlet port, a sample injection outlet port, and a spacer including a separation channel cavity defining at least a portion of the separation channel, a sample injection inlet cavity configured to be in fluid contact with the separation channel and located substantially beneath the sample injection inlet port, a sample injection outlet cavity configured to be in fluid contact with the separation channel and located substantially beneath the sample injection outlet port, such that the injection inlet and outlet paths are configured to define an injection channel that is essentially perpendicular to the length of the separation channel spanning the width of the separation channel cavity.

The disclosure provides devices and methods for controlled assembly of colloidal particles. A medium with colloidal particles having a charged surface is placed in physical contact with an electrically conductive material (e.g., an ITO coating). An external light source directs light towards the electrically conductive material, thus driving the colloidal particles from a first non-assembled state to a second assembled state, which may thus create organized colloidal crystals or alternatively predetermined void regions. Assembly of the colloids can be achieved with no external electric fields or external magnetic fields. Moreover, the colloidal assembly is three-dimensional, occurs rapidly, and is entirely reversible and reconfigurable based on controlling the light applied to the electrically conductive material.

The disclosure provides devices and methods for controlled assembly of colloidal particles. A medium with colloidal particles having a charged surface is placed in physical contact with an electrically conductive material (e.g., an ITO coating). An external light source directs light towards the electrically conductive material, thus driving the colloidal particles from a first non-assembled state to a second assembled state, which may thus create organized colloidal crystals or alternatively predetermined void regions. Assembly of the colloids can be achieved with no external electric fields or external magnetic fields. Moreover, the colloidal assembly is three-dimensional, occurs rapidly, and is entirely reversible and reconfigurable based on controlling the light applied to the electrically conductive material.

A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.

A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.

A biosensor system includes an array of biosensors with a plurality of electrodes situated proximate the biosensor. A controller is configured to selectively energize the plurality of electrodes to generate a DEP force to selectively position a test sample relative to the array of biosensors.