Systems, methods, and devices are described herein for identifying, monitoring, isolating, or selecting a cell having a predefined characteristic in a mixed population of cells utilizing a combination of any one or more of iDEP, a region of localized field enhancement, a variable frequency electric field, a wide bandwidth amplifier, and/or an imaging apparatus.

The object of the present invention is to provide a separation method and a separation apparatus for carbon nanotubes capable of separating a mixture of carbon nanotubes in a highly efficient, inexpensive and simple manner. The present invention relates to a carbon nanotube separation method comprising: a step of preparing a dispersion liquid including a mixture of two or more types of carbon nanotubes having different zeta potentials; a step of introducing the dispersion liquid into a flow path formed between a first electrode having holes for allowing the dispersion liquid to pass therethrough, and a second electrode arranged so as to face the first electrode; a step of applying a DC voltage to the first electrode and the second electrode while the dispersion liquid is flowing through the flow path; and, a step of continuously collecting a dispersion liquid including carbon nanotubes separated to a first electrode side upon application of the voltage from an opposite side to the flow path with respect to the first electrode, and at the same time, continuously collecting a dispersion liquid including carbon nanotubes separated to a second electrode side from a downstream side of the flow path.

A device for sorting bio-particles by image-manipulated electric force includes a first substrate, a second substrate, a fluidic channel, one or more photosensitive layers and an inlet hole. The first substrate has a first conductive electrode, and the second substrate has a second conductive electrode. The second conductive electrode is disposed opposite the first conductive electrode. The fluidic channel is disposed between the first conductive electrode and the second conductive electrode. The photosensitive layer is conformally disposed on at least one of the surfaces of the first conductive electrode and the second conductive electrode. The inlet hole is disposed in the first conductive electrode and the first substrate, where the inlet hole includes a first opening close to the fluidic channel and a second opening away from the fluidic channel, and the surface area of the first opening is greater than the surface area of the second opening.

A method for assembling multi-component nano-structures that includes dispersing a plurality of nano-structures in a fluid medium, and applying an electric field having an alternating current (AC) component and a direct current (DC) component to the fluid medium containing the plurality of nano-structures. The electric field causes a first nano-structure from the plurality of nano-structures to move to a predetermined position and orientation relative to a second nano-structure of the plurality of nano-structures such that the first and second nano-structures assemble into a multi-component nano-structure.

A 3-dimensional PDMS cell sorter having multiple passages in a PDMS layer that follow the same path in a DEP separation region and that are in fluid communication with each other within that region. The passages may differ in width transverse to the flow direction within the passages. Flat plates may sandwich the PDMS layer; each plate may have a planar electrode used to generate a DEP field within a sample fluid flowed within the passages. The DEP field may concentrate target cells or particulates within one of the passages within the DEP separation region. The passages may diverge after the DEP-separation region, leaving one passage with a high concentration of target cells or particulates. Techniques for manufacturing such structures, as well as other micro-fluidic structures, are also provided.

An inverted microwell provides rapid and efficient microanalysis system and method for screening of biological particles, particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.

A method and apparatus is described that uses a device that presents a surface of a dielectric material to a flowing or circulating fluid that results in charge being created at a boundary layer of the dielectric where it contacts the fluid by ion exchange and charging of compounds, for example insoluble dielectric particles such as colloids, and using at least one control impedance to control an impressed current in the fluid caused by the fluid flowing through the device and to cause suspended particles in the fluid to be charged, whereby the charge of the suspended particles is then neutralised causing coagulation of the particles as suspended insoluble particles.

A system for extracting metals (e.g. precious metals or dangerous metals) from a substrate material such as sludge from a lake bed or sewage treatment facility includes processing the substrate material and metals by exposing the substrate material and metals to the plasma of an electric arc. Then, the exposed substrate material and metals are passed through an electrically charged collection grid in which the metals, now electrically charged, are attracted to the collection grid and hold to the collection grid and the substrate material exits the collection grid with less concentrations (or none) of the metals. In some embodiments, in addition to recovering the metals (e.g. precious metals, dangerous metals, etc.), a flammable gas is produced.

A system for extracting metals (e.g. precious metals or dangerous metals) from a substrate material such as sludge from a lake bed or sewage treatment facility includes processing the substrate material and metals by exposing the substrate material and metals to the plasma of an electric arc. Then, the exposed substrate material and metals are passed through an electrically charged collection grid in which the metals, now electrically charged, are attracted to the collection grid and hold to the collection grid and the substrate material exits the collection grid with less concentrations (or none) of the metals. In some embodiments, in addition to recovering the metals (e.g. precious metals, dangerous metals, etc.), a flammable gas is produced.

A cell sorting apparatus includes a flow channel through which fluid including cells flows, an electric-field application section capable of applying an electric field having a gradient in a direction different from the flowing direction of the fluid at a first position on the flow channel in accordance with a cell sorting signal requesting an operation to sort the cells, and a flow splitting section configured to split the cells changing their flowing directions due to a dielectrophoretic force caused by application of the electric field at a second position on the downstream side of the first position on the flow channel.