DNA constructs as well as methods for the production of unicellular organisms capable of producing hydrogen peroxide resistance proteins are disclosed. DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular organisms for the expression of hydrogen peroxide production proteins are also disclosed. In addition, DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular organisms for the expression of hydrogen peroxide resistance and hydrogen peroxide production proteins are disclosed.

DNA constructs as well as methods for the production of unicellular organisms capable of producing hydrogen peroxide resistance proteins are disclosed. DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular organisms for the expression of hydrogen peroxide production proteins are also disclosed. In addition, DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular organisms for the expression of hydrogen peroxide resistance and hydrogen peroxide production proteins are disclosed.

In an embodiment of the invention, aqueous growth medium in a pond can be used to grow algae which can be pumped to a primary dewatering device where the algae can be separated from the harvested growth media based on the flow of the harvested growth media and gravity. The flow through the primary de-watering device can be optimized to maintain log phase growth in the pond, while minimizing the pumping cost and maximizing the concentration of total solids in the primary de-watered algae.

The present disclosure relates to the field of algal cultivation and biofuels. Particularly, the present disclosure relates to a method of enhancing lipid production during algal culturing. Particularly, the present disclosure relates to a method of enhancing neutral lipid and total lipid production by maintaining algae in a thin layer cultivation system and exposing said algae maintained in the thin layer cultivation system to infra-red (IR) radiation. Said method enhances lipid accumulation in algae, thereby increasing the yield of neutral lipids and total lipids. The method is simple, cost-effective in producing high quantities of algal-derived biofuels, requires shorter time duration for lipid induction and results in no or minimal reduction of biomass.

The present disclosure relates to the field of algal cultivation and biofuels. Particularly, the present disclosure relates to a method of enhancing lipid production during algal culturing. Particularly, the present disclosure relates to a method of enhancing neutral lipid and total lipid production by maintaining algae in a thin layer cultivation system and exposing said algae maintained in the thin layer cultivation system to infra-red (IR) radiation. Said method enhances lipid accumulation in algae, thereby increasing the yield of neutral lipids and total lipids. The method is simple, cost-effective in producing high quantities of algal-derived biofuels, requires shorter time duration for lipid induction and results in no or minimal reduction of biomass.

A photobioreactor (PBR) cell comprising a riser portion in the form of a tube; a downcomer portion in the form of a tube; a means of joining the riser portion and the downcomer portion to allow liquid flow; and an aeration means.