Provided herein are systems and methods for biological sample concentration, purification, and fractionation of biological samples. The systems can comprise one or more containment devices for a biological sample connected to a shaft; a handheld motor source connected to the shaft and configured to modulate spinning the one or more containment devices along an axis of the one or more containment devices.

Provided herein are systems and methods for biological sample concentration, purification, and fractionation of biological samples. The systems can comprise one or more containment devices for a biological sample connected to a shaft; a handheld motor source connected to the shaft and configured to modulate spinning the one or more containment devices along an axis of the one or more containment devices.

The invention provides devices for improving assay protocol compliance, reproducibility, and sensitivity. In particular, the devices of the present invention are tailored to certain single-cell sequencing assays, including single-cell RNA sequencing assays that utilize pre-templated instant partitions (PIPs) templates, wherein such devices provide improved means of holding tubes stationary while performing sample preparation, provide reproducible volume removal from tubes, allow for improved magnetic separation of analytes from buffers, and provide reproducible centrifugation of tubes.

A closed loop extraction system for extracting desired components from raw plant matter and method of use is disclosed. The closed loop extraction system features a solvent source, a chill tank, a wash tank, a rinse tank, a centrifuge, and a feed tank all in fluid communication via a series of interconnected pump systems. The method of extracting the desired products from raw plant matter uses this system to perform the extraction with a reduced amount of solvent in an energy-efficient manner.

The present invention relates, in part, to methods, systems and processes for large-scale purification of mRNA using a filtering centrifuge operating at lower gravitational forces. The invention also relates to compositions of purified mRNA and uses thereof.

The present invention relates, in part, to methods, systems and processes for large-scale purification of mRNA using a filtering centrifuge operating at lower gravitational forces. The invention also relates to compositions of purified mRNA and uses thereof.

A filter unit (10) for separation of particulate matter from particulate-laden liquid, the filter unit comprising: a chamber (12) defined by an upper axial end wall (14) and an opposing lower axial end wall (16) and a peripheral particle collection wall (18), the upper and lower axial end walls being spaced by the peripheral particle collection wall, the chamber being rotatable about an axis of rotation (30) so as to impart rotational motion to the liquid; an inlet (23) for delivering particulate-laden liquid into the chamber (12); an outlet (24) in the upper or lower axial end wall for discharging filtered liquid from the chamber; a flow path (22) from the inlet to the outlet; wherein the flow path includes a radial component from the inlet to the peripheral particle collection wall and an axial component along the peripheral particle collection wall.

A filter unit (10) for separation of particulate matter from particulate-laden liquid, the filter unit comprising: a chamber (12) defined by an upper axial end wall (14) and an opposing lower axial end wall (16) and a peripheral particle collection wall (18), the upper and lower axial end walls being spaced by the peripheral particle collection wall, the chamber being rotatable about an axis of rotation (30) so as to impart rotational motion to the liquid; an inlet (23) for delivering particulate-laden liquid into the chamber (12); an outlet (24) in the upper or lower axial end wall for discharging filtered liquid from the chamber; a flow path (22) from the inlet to the outlet; wherein the flow path includes a radial component from the inlet to the peripheral particle collection wall and an axial component along the peripheral particle collection wall.

A container set including: a filter unit; and a closing unit that is attachable to and detachable from the filter unit. The filter unit includes: a housing having a sample introduction opening provided at one end, and a liquid discharging opening provided at the second end; and a filter fixed to the housing. The housing has a first joining part, and the closing unit has a second joining part configured to be joinable with the first joining part. The first joining part and the second joining part are configured such that the liquid discharging opening is spatially closed in a state in which the two joining parts are joined together. A space between the closing unit and the filter is made into a positive pressure relative to a pressure in the sample holding space by pushing the filter unit and the closing unit such that the distance therebetween is shortened.

A container set including: a filter unit; and a closing unit that is attachable to and detachable from the filter unit. The filter unit includes: a housing having a sample introduction opening provided at one end, and a liquid discharging opening provided at the second end; and a filter fixed to the housing. The housing has a first joining part, and the closing unit has a second joining part configured to be joinable with the first joining part. The first joining part and the second joining part are configured such that the liquid discharging opening is spatially closed in a state in which the two joining parts are joined together. A space between the closing unit and the filter is made into a positive pressure relative to a pressure in the sample holding space by pushing the filter unit and the closing unit such that the distance therebetween is shortened.