A mechanical seal device for a centrifugal separator includes a first seal ring including a first seal surface and a second seal ring including a second seal surface. The first and second seal surfaces are configured to face each other to form a seal, and at least one channel for a cooling fluid is arranged in the first seal ring. The at least one channel includes an inlet and an outlet. The outlet of the at least one channel is arranged at the first seal surface of the first seal ring. A centrifugal separator includes the mechanical seal device.

A shot peening apparatus includes a first tank. The first tank includes a swirl flow generating mechanism and a suction port. The swirl flow generating mechanism swirls a waste liquid in the circumferential direction. The suction port is disposed at the axial center of the first tank. The suction port opens toward a bottom wall and suctions the waste liquid. A plurality of blade members are provided inside the first tank. The plurality of blade members hinders the waste liquid from swirling in the first tank.

A shot peening apparatus includes a first tank. The first tank includes a swirl flow generating mechanism and a suction port. The swirl flow generating mechanism swirls a waste liquid in the circumferential direction. The suction port is disposed at the axial center of the first tank. The suction port opens toward a bottom wall and suctions the waste liquid. A plurality of blade members are provided inside the first tank. The plurality of blade members hinders the waste liquid from swirling in the first tank.

A method for separating a cell culture mixture utilizes a centrifugal separator including a stationary frame, a rotatable assembly and a drive unit for rotating the rotatable assembly relative the frame around a vertical axis of rotation. The rotatable assembly includes a rotor casing enclosing a separation space in which a stack of separation discs is arranged to rotate around an axis of rotation. The rotor casing includes a mechanically hermetically sealed inlet for supply of the cell culture mixture to the separation space and first and second mechanically hermetically sealed liquid outlets. The second mechanically hermetically sealed outlet is arranged at an axial end of the rotor casing and arranged so that the separated cell phase is discharged at the rotational axis.

Provided herein are systems, devices and methods for harvesting biological cells with a centrifuge. A biological cell harvesting centrifuge apparatus may be provided with a rectangularly shaped base and 21 laterally spaced apart upright supports. Each upright support may extend vertically upward from the base and have a hollow core. Each upright support may be configured to slidably receive and support either one, two or four vertical edges of either one, two or four 15 mL micro bioreactor vessels. The 21 upright supports may be arranged in a 3?7 array such that they form a 2?6 array of receptacles configured to slidably receive and support 12 of the bioreactor vessels. The upright supports may be configured to support at least 80% of the vertical edges of the bioreactor vessels.

Provided herein are systems, devices and methods for harvesting biological cells with a centrifuge. A biological cell harvesting centrifuge apparatus may be provided with a rectangularly shaped base and 21 laterally spaced apart upright supports. Each upright support may extend vertically upward from the base and have a hollow core. Each upright support may be configured to slidably receive and support either one, two or four vertical edges of either one, two or four 15 mL micro bioreactor vessels. The 21 upright supports may be arranged in a 3?7 array such that they form a 2?6 array of receptacles configured to slidably receive and support 12 of the bioreactor vessels. The upright supports may be configured to support at least 80% of the vertical edges of the bioreactor vessels.

A centrifugal structure member of a microfluidic chip and a centrifuge are provided. The centrifugal structure member of a microfluidic chip includes: a connecting portion (10) configured to connect to a rotor of a centrifuge; a support portion (20) fixedly connected or integrally formed with the connecting portion (10), and having an inclined outer surface forming an included angle with the rotation axis (50) of the rotor of the centrifuge; and a mounting portion (30) connected with or formed on the inclined outer surface, and configured to detachably mount the microfluidic chip (40) on the support portion (20).

An inflow apparatus includes an inlet opening for introducing a starting product into the inflow apparatus, at least one first outlet opening and one second outlet opening different from the first outlet opening for feeding the starting product from the inflow apparatus into the filling zone. The inflow apparatus additionally includes a connection device via which the inlet opening is in flow communication with the first outlet opening and with the second outlet opening. To ensure a homogeneous distribution of the starting product in the filling zone in the peripheral direction, the connection device includes a first passage and a second passage different from the first passage, with the first outlet opening being in flow communication with the inlet opening via the first passage and the second outlet opening being in flow communication with the inlet opening via the second passage.

An inflow apparatus includes an inlet opening for introducing a starting product into the inflow apparatus, at least one first outlet opening and one second outlet opening different from the first outlet opening for feeding the starting product from the inflow apparatus into the filling zone. The inflow apparatus additionally includes a connection device via which the inlet opening is in flow communication with the first outlet opening and with the second outlet opening. To ensure a homogeneous distribution of the starting product in the filling zone in the peripheral direction, the connection device includes a first passage and a second passage different from the first passage, with the first outlet opening being in flow communication with the inlet opening via the first passage and the second outlet opening being in flow communication with the inlet opening via the second passage.

A centrifugal separator includes a frame and a rotating part including a spindle and a centrifuge rotor enclosing a separation space. An inlet channel provides fluid communication into the separation space and includes a rotary channel part, a frame channel part, and a first seal at the interface between the rotary and frame channel parts. An outlet channel provides fluid communication out from the separation space and includes a rotary channel part, a frame channel part, and a second seal at the interface between the rotary and frame channel parts. The inlet and outlet channels are arranged concentrically with each other. A forcing device is provided to generate a leak flow through one of the first and second seals in a first direction from the outlet channel to the inlet channel and to counteract leakage in the opposite direction.