The present invention relates to a system and method of cultivating Cordyceps, specifically, provided herein is a system of cultivating Cordyceps comprising: a Hepialus moth trapping device; a Hepialus moth eggs screening device, wherein the device is connected with the Hepialus moth trapping device; an ascospores-collecting device; a mycelia-preparing device; a conidia-preparing device; an infection device, wherein the device is connected with the ascospores-collecting device, the mycelia-preparing device and the conidia-preparing device; and a feeding device, wherein the device is connected with the Hepialus moth eggs screening device and the infection device. And also provided is a method of using the system to cultivate Cordyceps.

A habitat determination method includes: calculating, by a computer, a first indicator, which is a number of trees per predetermined area with respect to a tree which allows tree mammals to inhabit; and determining, by the computer, according to the calculated first indicator and a characteristic of each tree mammal, whether or not each tree mammal is inhabitable.

A habitat determination method includes: calculating, by a computer, a first indicator, which is a number of trees per predetermined area with respect to a tree which allows tree mammals to inhabit; and determining, by the computer, according to the calculated first indicator and a characteristic of each tree mammal, whether or not each tree mammal is inhabitable.

Human or humanized tissues and organs suitable for transplant are disclosed herein. Gene editing of a host animal provides a niche for complementation of the missing genetic information by donor stem cells. Editing of a host genome to knock out or debilitate genes responsible for the growth and/or differentiation of a target organ and injecting that animal at an embryo stage with donor stem cells to complement the missing genetic information for the growth and development of the organ. The result is a chimeric animal in which the complemented tissue (human/humanized organ) matches the genotype and phenotype of the donor. Such organs may be made in a single generation and the stem cell may be taken or generated from the patient's own body. As disclosed herein, it is possible to do so by simultaneously editing multiple genes in a cell or embryo creating a niche for the complemented tissue. Multiple genes can be targeted for editing using targeted nucleases and homology directed repair (HDR) templates in vertebrate cells or embryos.

Improved molecular breeding methods include a method in which an association data set is developed by associating the phenotypes of a broad population of individuals with the individual genotypes. The association data set is used in conjunction with a growth model in order to select breeding pairs likely to generate offspring with one or more desirable traits.

Improved molecular breeding methods include a method in which an association data set is developed by associating the phenotypes of a broad population of individuals with the individual genotypes. The association data set is used in conjunction with a growth model in order to select breeding pairs likely to generate offspring with one or more desirable traits.

An artificial feeding system for maintenance and growth of hematophagous insects is disclosed to comprise a blood-free diet formulation and a feeding device. The blood-free diet formulation comprises effective amounts of a protein source and a carbohydrate source, wherein the blood-free diet formulation is suitable for egg production and colony maintenance of hematophagous insects. The feeding device comprises a diet reservoir for receiving the blood-free diet formulation and a feeding platform for hematophagous insects to feed the blood-free diet formation from the diet reservoir.

An object of the present invention is to provide a novel medical application for use in regenerative medicine that uses pluripotent stem cells (Muse cells). The present invention provides a cell preparation for treating myocardial infarction, and particularly serious massive myocardial infarction and heart failure associated therewith, that contains pluripotent stem cells positive for SSEA-3 isolated from biological mesenchymal tissue or cultured mesenchymal cells. The cell preparation of the present invention is based on a cardiac tissue regeneration mechanism by which Muse cells are made to selectively accumulate in damaged myocardial tissue and differentiate into cardiac muscle in that tissue as a result of intravenous administration of Muse cells to a subject presenting with the aforementioned disorders.

A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.

The present invention discloses a method for identifying Wuzhishan miniature pig inbred line by using 145 SNPs. The method for auxiliarily identifying whether a pig to be tested is Wuzhishan miniature pig inbred line, provided by the present invention, comprises the following steps: testing genotypes based on 145 SNP sites of the pig to be tested; if all standards of (1) to (145) are satisfied, the pig to be tested is a candidate for Wuzhishan miniature pig inbred line; if all the standards of the above (1) to (145) are not satisfied, the pig to be tested is a candidate for non-Wuzhishan miniature pig inbred line.