Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

A container for exchanging fluids using pressurized air has a cylindrical tank with caps and seals at the tank's opposite ends. Grooves in the caps hold the seals, and the caps move between unsealed and sealed arrangements using a tie rod engaging threaded bores in the caps. When sealed, the seals are radially compressed between the flange and the tank's interior sidewall. The tie rod connects to threaded bores at the caps' centers. Rotating the caps relative to the tank axially moves the caps between the sealed and unsealed arrangements. The top cap has a fill port with a threaded plug. A process tube connects to either the outlet port for the fluid when the top cap has the outlet or the inlet port for the pressurized air when the bottom cap has the inlet. A float valve connects to the process tube's end for fluid flowing through the outlet.

A combination set of eco-friendly recycling beverage container includes a container unit and a recycling storage unit. The container unit includes a soft part that has softness that folds and bends more easily than plastic or glass and stores a beverage therein, an inlet support part coupled around a beverage outlet at an upper end of the soft part, and a bottom support part coupled to a lower end of the soft part. The recycling storage unit is configured to accommodate and bind the container unit, the recycling storage unit having a multi-stage overlapping structure in which an overall height is reduced depending on a degree of reduced amount of the beverage contained in the container unit.

A packaging device and a manufacturing method thereof are provided. The packaging device includes: a bottom plate (1); a side plate (2) fixed to a surface of the bottom plate (1), the bottom plate (1) and the side plate (2) forming a containing cavity through joint enclosure; and a supporting piece (3) in the bottom plate (1). The bottom plate (1) completely wraps the supporting piece (3). An extending direction of the supporting piece (3) is roughly parallel to the surface of the bottom plate (1). A material of the supporting piece (3) is different from that of the bottom plate (1), and a strength of the supporting piece (3) is greater than that of the bottom plate (1).

A device for collecting split wood, having a plurality of bar elements, which each have a first and a second longitudinal portion, and a plurality of connecting elements, which connect the bar elements movably to one another. The second longitudinal portion of each bar element extends in a curved manner and the first longitudinal portion extends in a straight manner. The connecting elements cooperate with the second longitudinal portions, and a clamping element, which cooperates with the first longitudinal portions, is designed to firmly clamp the bar elements to a chopping block by their first longitudinal portions.

The present disclosure provides for a reversible and adjustable stopper assembly comprised of an upper frame, L-bracket, connector portion and stopping member. The stopper can be configured to be secured to at least two main types of press brakes. The stopping portion of the stopper is also reversible, allowing for less sheet metal to be inserted into the press brake, if desired. The stopper assembly can be slid in a horizontal manner, relative to the press brake to preserve the measurement of the sheet metal from one end of the press brake to the other.

The utility model relates to drinking vessels, more specifically to a disposable container for hot and cold beverages. The technical result lies in the creation of a disposable cup made of a multi-layered material based on foamed primary polyethylene terephthalate and/or secondary polyethylene terephthalate (obtained from polyethylene terephthalate waste), which is easy to produce and fit for use. The essence of the invention consists in that a disposable cup has a lateral wall, in the form of a rotation body, and a base, which are fastened to each other, wherein a flanged edge is formed in the upper part of the lateral wall, the lateral wall and the base are multi-layered and comprise a layer of foamed polyethylene terephthalate with a density of from 100 kg/m.sup.3 to 900 kg/m.sup.3 and a characteristic viscosity of from 0.5 dl/g to 1.0 dl/g, which is connected to a polyethylene layer by fusion.

A user friendly twist and lock telescoping tubular package includes tubular package pieces with built-in visual cues assisting a user in the proper assembly and operation of the package, and with improved engagement features opposing improper operation of the package while facilitating proper use of the package. Improved locking features are more resistant to inadvertent unlocking or repositioning of the package. Support and stability features enhance the locking connections and improve reliability of the locking connections.

A tamper-indicating assembly for a drum enclosure assembly is provided. The tamper-indicating assembly includes a tamper-indicating device that defines a cavity sized to receive and surround at least a portion of the closure bolt, the first flanged end, and the second flanged end to prevent movement of the closure bolt. A tab extends radially outward from the tamper-indicating device with respect to an axial centerline of the tamper-indicating assembly. The tamper-indicating assembly further includes a pin non-removably coupled to the tamper-indicating device. The pin extends through the tamper-indicating device and across the cavity such that the tamper-indicating device and the pin collectively surround the closure bolt. The exemplary tamper-indicating device described herein includes features that facilitate the robotic application of the tamper-indicating device to a drum enclosure.

A user friendly twist and lock telescoping tubular package includes tubular package pieces with built-in visual cues assisting a user in the proper assembly and operation of the package, and with improved engagement features opposing improper operation of the package while facilitating proper use of the package. Improved locking features are more resistant to inadvertent unlocking or repositioning of the package. Support and stability features enhance the locking connections and improve reliability of the locking connections.