The present disclosure relates to systems and methods for producing an extruded protein product. In particular, a system for making an extruded protein product using a system that includes a die including channel having a transverse cross section that is a continuous loop along at least a portion of the length of the die is disclosed.

A method for producing a part in the form of a solid block from a natural material in particulate form containing scleroproteins. A phase of heating the natural material, under compression at a pressure greater than or equal to 30 MPa, to a temperature greater than or equal to the denaturation temperature of the scleroproteins contained in the material. A phase of cooling the material thus obtained to a temperature less than 100 C., while maintaining the compression during at least a part of the cooling phase.

A cardiac patch for treatment of a mammalian heart including perfusable vessels embedded integratedly between two layers of anisotropically oriented myocardial fibers. The cardiac patch is made using a dual 3D bioprinting technique using stereolithography to form an anisotropic construct and extrusion printing to form perfusion vessels. A nutrient and oxygen containing media can be provided within the perfusion vessels for growth of cells in the cardiac patch. The technique permits larger patches to be made for the treatment of cardiac damage in both small and large mammalian hearts.

Methods for making an osteoimplant are provided. In one embodiment the method includes applying a mechanical force to an aqueous slurry of insoluble collagen fibers to entangle the insoluble collagen fibers so as to form a semi-solid mass of entangled insoluble collagen fibers; and lyophilizing the semi-solid mass of entangled collagen fibers to form the osteoimplant. An osteoimplant containing entangled insoluble collagen fibers is also provided.

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

A biomimetic microtube and a preparation method thereof are provided. A coaxial pipe is used to form a biomimetic microtube having a core solution and a wall surrounding the core solution. In the preparation method, some various processing methods can be used to increase the roughness, porosity, and hardness of the wall of the biomimetic microtube.

The invention relates to a method (100) for producing a three-dimensional object (10), having the following steps: a printing structure (11), which defines an interior (12), is produced (110) from a printing material (21) by means of 3-D printing; a filling material (22), which comprises at least one liquid or pasty monomer (23), is introduced (120) into the interior (12); the monomer (23) is polymerized (130) to form a polymer (24). The invention further relates to a 3-D printer (30) for performing the method (100), wherein a first printing head (31) for the printing material (21) and a second printing head (32) for the filling material (22) are provided, wherein the outlet opening (32a) of the second printing head (32) for the filling material (22) has a cross-sectional area that is greater than that of the outlet opening (31a) of the first printing head (31) for the printing material (21) by a factor of at least 2, preferably by a factor of at least 5, and/or a base plate (33) is provided, on which the printing structure (11) should be constructed, wherein the base plate (33) has a feed-through (34) for the filling material (22), which feed-through can be connected, on the side facing away from the printing structure (11), to a pressurized source (26) for the filling material (22).

Methods for making an osteoimplant are provided. In one embodiment the method includes applying a mechanical force to an aqueous slurry of insoluble collagen fibers to entangle the insoluble collagen fibers so as to form a semi-solid mass of entangled insoluble collagen fibers; and lyophilizing the semi-solid mass of entangled collagen fibers to form the osteoimplant. An osteoimplant containing entangled insoluble collagen fibers is also provided.

The present invention relates to biomaterial in the form of dispersion of cellulose nanofibrils with extraordinary shear thinning properties which can be converted into desire 3D shape using 3D Bioprinting technology. In this invention cellulose nanofibril dispersion, is processed through different mechanical, enzymatic and chemical steps to yield dispersion with desired morphological and rheological properties to be used as bioink in 3D Bioprinter. The processes are followed by purification, adjusting of osmolarity of the material and sterilization to yield biomaterial which has cytocompatibility and can be combined with living cells. Cellulose nanofibrils can be produced by microbial process but can also be isolated from plant secondary or primary cell wall, animals such as tunicates, algae and fungi. The present invention describes applications of this novel cellulose nanofibrillar bioink for 3D Bioprinting of tissue and organs with desired architecture.

Described herein is a 3D-printed scaffold comprising a peptide-polymer conjugate, the peptide-polymer conjugate having the structure: X-Y-Z-Y-X, wherein X is a biologically active peptide, Y is a linker moiety, and Z is a biocompatible and biodegradable polymer.