Described herein are apparatuses, systems, and methods for fabricating tissue constructs, such as by fabricating perfusable tissue constructs by embedding a sacrificial material into a composite matrix yield stress support bath. A composite matrix bath can include a microgel filler and a hydrogel precursor. An extrusion tip can be used for embedded printing of perfusable tissue constructs by disposing sacrificial material into the composite matrix bath while the extrusion tip travels along a predefined course through the composite matrix bath. This sacrificial material can be the printed tissue construct or can be removed to render the matrix bath a perfusable tissue construct. The composite matrix bath can include acellular or cell-laden hydrogels. The sacrificial material can include a salt and a physiological buffer or a non-cytotoxic porogen material. The hydrogel precursor can include at least one of gellan and gelatin. Cross-linking can be carried out chemically, thermally, enzymatically, or physically.

The present invention relates to bioinks based on undenatured collagen, kits containing the same, and methods of printing three-dimensional structures using the bioinks.

The present invention relates to biomaterial in the form of dispersion of cellulose nanofibrils with extraordinary shear thinning properties which can be converted into desire 3D shape using 3D Bioprinting technology. In this invention cellulose nanofibril dispersion, is processed through different mechanical, enzymatic and chemical steps to yield dispersion with desired morphological and rheological properties to be used as bioink in 3D Bioprinter. The processes are followed by purification, adjusting of osmolarity of the material and sterilization to yield biomaterial which has cytocompatibility and can be combined with living cells. Cellulose nanofibrils can be produced by microbial process but can also be isolated from plant secondary or primary cell wall, animals such as tunicates, algae and fungi. The present invention describes applications of this novel cellulose nanofibrillar bioink for 3D Bioprinting of tissue and organs with desired architecture.

The present invention relates to biomaterial in the form of dispersion of cellulose nanofibrils with extraordinary shear thinning properties which can be converted into desire 3D shape using 3D Bioprinting technology. In this invention cellulose nanofibril dispersion, is processed through different mechanical, enzymatic and chemical steps to yield dispersion with desired morphological and rheological properties to be used as bioink in 3D Bioprinter. The processes are followed by purification, adjusting of osmolarity of the material and sterilization to yield biomaterial which has cytocompatibility and can be combined with living cells. Cellulose nanofibrils can be produced by microbial process but can also be isolated from plant secondary or primary cell wall, animals such as tunicates, algae and fungi. The present invention describes applications of this novel cellulose nanofibrillar bioink for 3D Bioprinting of tissue and organs with desired architecture.

The present invention relates to a method of producing a coherent growth substrate product formed of man-made vitreous fibres (MMVF), comprising the steps of (i) providing MMVF; (ii) providing an uncured binder composition; (iii) providing an additive; (iv) forming a mixture of the MMVF, the uncured binder composition and the additive; (v) curing the uncured binder composition in the mixture to form the coherent growth substrate product; wherein the uncured binder composition comprises at least one hydrocolloid; and wherein the additive is a micro-organism, fungus, biologically-active additive or combination thereof.

The present disclosure provides a composition comprising a hierarchical opal that exhibits structural color when exposed to incident electromagnetic radiation. The hierarchical opal comprises nanoscale periodic cavities separated by a lattice constant, and includes a surface having grooves. The grooves may form a diffractive optical element on the surface of the hierarchical opal, such as a diffuser, a diffraction grating, a beamsplitter, a beam displacement opic, a Fresnel lens, and a micro lens, among others.

A process for the production of a three-dimensional particulate structure embedded in a body formed of a hydrogel matrix, comprising the steps of forming a layer of a hydrogel matrix having a particulate substructure embedded therein by subjecting a suspension of particulates in a layer of a hydrogel matrix precursor to standing acoustic waves (SAWs) to spatially partition the particulates within the layer of hydrogel precursor into a particulate substructure and allowing the hydrogel precursor to solidify such as to form the layer of a hydrogel matrix having a particulate substructure embedded therein, and repeating the process until the three-dimensional particulate structure embedded in a body formed of a hydrogel matrix is formed.

The present invention relates to an aqueous binder composition for mineral fibers comprising at least one hydrocolloid, at least one fatty acid ester of glycerol.

The invention relates to a method of extruding sleeves extruded of collagen for encasing sausages, wherein a collagen fluid is fed to an extrusion head; the collagen fluid is guided through the extrusion head; and the collagen fluid is discharged from the extrusion head. The invention also relates to an extrusion head for performing such a process as well as a co-extrusion sausage line. Finally also the sleeve extruded with this method or devices is part of the invention.

Disclosed herein are improved cross-linked resilin compositions and methods of making these improved compositions. These include new methods of cross-linking resilin compositions, use of polar nonaqueous solvents for adjusting material properties of cross-linked resilin solid compositions, and resilin foam compositions and methods of making the same.