A system for and method of reconstituting post mortem circulation is provided. Certain methods include simulating natural clotting cascades at wound sites while preventing clotting in reservoirs and within the cadaver. Clot prevention in the reservoir and the cadaver are accomplished with an anticoagulant and a dispersant, respectively. Simulation of clotting at the wound site is accomplished by diluting cadaver fluid with reservoir fluid, thereby diluting the dispersant. The cadaver fluid contains excess calcium for resisting the anticoagulant of the reservoir fluid. Certain methods of the present invention also include reanimating a heart, attaching foreign cadaveric circulatory branches to other cadaveric tissue, enabling mobile cadaveric perfusion, and utilizing a water bath with gravity-feed perfusion.

A system for and method of reconstituting post mortem circulation is provided. Certain methods include simulating natural clotting cascades at wound sites while preventing clotting in reservoirs and within the cadaver. Clot prevention in the reservoir and the cadaver are accomplished with an anticoagulant and a dispersant, respectively. Simulation of clotting at the wound site is accomplished by diluting cadaver fluid with reservoir fluid, thereby diluting the dispersant. The cadaver fluid contains excess calcium for resisting the anticoagulant of the reservoir fluid. Certain methods of the present invention also include reanimating a heart, attaching foreign cadaveric circulatory branches to other cadaveric tissue, enabling mobile cadaveric perfusion, and utilizing a water bath with gravity-feed perfusion.

In at least one embodiment the present invention provides a mobile storage system including a container having a first end, second end, first side, second side, ceiling and floor defining a rectangular prismatic cavity, an access door that corresponds with an access opening positioned on at least one of the longitudinal sides between the first end and the second and of the container, a climate control unit for cooling the container, a rack unit positioned within one of the first end and the second end of the container and adapted to slidably yet releasably receive a tray in a stowed position, and a mobile stretcher adjustable from a first height to a least a second height and adapted to securely yet releasably receive the tray.

In at least one embodiment the present invention provides a mobile storage system including a container having a first end, second end, first side, second side, ceiling and floor defining a rectangular prismatic cavity, an access door that corresponds with an access opening positioned on at least one of the longitudinal sides between the first end and the second and of the container, a climate control unit for cooling the container, a rack unit positioned within one of the first end and the second end of the container and adapted to slidably yet releasably receive a tray in a stowed position, and a mobile stretcher adjustable from a first height to a least a second height and adapted to securely yet releasably receive the tray.

A human tissue isolation pouch for use with a sealer machine to indirectly seal human tissue medical pouches while avoiding cross contamination is herein disclosed. The human tissue isolation pouch comprises a rigid frame supporting a high temperature material and having one or more magnets for removably attaching the human tissue isolation pouch to a sealer machine.

During use a medical pouch, which may contain a human tissue sample, is inserted within the human tissue isolation pouch. The jaws of the sealer machine clamp down directly on the high temperature material, thus indirectly heating and sealing the medical pouch inside the human tissue isolation pouch. The sealing machine jaws do not physically contact the medical pouch at any time during the sealing process, and in this way contamination of the sealer machine (and cross contamination of medical pouches from distinct donor cases) is avoided. The rigid frame of the human tissue isolation pouch may be sterilized after each donor event. And the containment bag formed of a high temperature material may be disposed of after each donor event.

A human tissue isolation pouch for use with a sealer machine to indirectly seal human tissue medical pouches while avoiding cross contamination is herein disclosed. The human tissue isolation pouch comprises a rigid frame supporting a high temperature material and having one or more magnets for removably attaching the human tissue isolation pouch to a sealer machine.

During use a medical pouch, which may contain a human tissue sample, is inserted within the human tissue isolation pouch. The jaws of the sealer machine clamp down directly on the high temperature material, thus indirectly heating and sealing the medical pouch inside the human tissue isolation pouch. The sealing machine jaws do not physically contact the medical pouch at any time during the sealing process, and in this way contamination of the sealer machine (and cross contamination of medical pouches from distinct donor cases) is avoided. The rigid frame of the human tissue isolation pouch may be sterilized after each donor event. And the containment bag formed of a high temperature material may be disposed of after each donor event.

A system for reconstituting circulation in a cadaver includes at least a pump, a fluid driven by the pump, and operative fluid connections between the pump and the cadaver. The system may be used to occlude one or more arterioles within the cadaver. The system may include a first circuit that creates a first fluid path with vessels of the cadaver, with first circuit having a first fluid conduit, a heater unit, a first pump, and a resistance device. The system may also include a second circuit that creates a second fluid path with vessels of the cadaver that are different from the vessels of the first circuit, with the second circuit having a second fluid conduit and a second pump. Alternately, the system may include a first circuit that creates a first fluid path with vessels of the cadaver and a second flow path that creates a second fluid path with the reservoir, where the first circuit includes a first conduit, a second connector, a pump, a reservoir, and a connector.

A system for reconstituting circulation in a cadaver includes at least a pump, a fluid driven by the pump, and operative fluid connections between the pump and the cadaver. The system may be used to occlude one or more arterioles within the cadaver. The system may include a first circuit that creates a first fluid path with vessels of the cadaver, with first circuit having a first fluid conduit, a heater unit, a first pump, and a resistance device. The system may also include a second circuit that creates a second fluid path with vessels of the cadaver that are different from the vessels of the first circuit, with the second circuit having a second fluid conduit and a second pump. Alternately, the system may include a first circuit that creates a first fluid path with vessels of the cadaver and a second flow path that creates a second fluid path with the reservoir, where the first circuit includes a first conduit, a second connector, a pump, a reservoir, and a connector.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

Described herein are methods of preserving a tissue of a subject, such as a brain or a portion thereof. The methods include washing the tissue by perfusing the tissue with a wash fluid comprising an aqueous solution; fixing the tissue by perfusing the tissue with a fixation fluid comprising an aldehyde; and cryoprotecting the tissue by perfusing the tissue with a cryoprotection fluid comprising a vitrification agent. The wash fluid, the fixation fluid, and/or the cryoprotection fluid can include a dye or a contrast agent to monitor perfusion of the fluid through the tissue. In certain embodiments, the cryoprotection fluid has a vitrification temperature of about ?80? C. or higher. The wash fluid can further include one or more of an ion channel blocker, a calcium chelator, a thrombolytic agent, an anti-platelet, a respiratory poison, or a synaptic poison. Also described herein are methods of analyzing the preserved tissue.