Disclosed herein are methods of producing substantially pathogen-free plants of the genus Cannabis and pathogen-free plants and clones produced therefrom. One embodiment of the method comprises pretreating a progenitor plant of the genus Cannabis, surface sterilizing a shoot segment of the pretreated plant with a bleach solution, excising a meristematic tip of the shoot segment, and transferring the meristematic tip into a culturing plate comprising a supplemented Murashige and Skoog culture medium for further culturing. The supplemented Murashige and Skoog culture medium can comprise benzyladenine, naphthaleneacetic acid, gibberellic acid, or a combination thereof.

Described are methods of field conditioning cereal crop pollen. A method of the present invention includes collecting fresh pollen and introducing the pollen to field conditioning conditions which regulate pollen moisture content. The field conditioning conditions may include a relative humidity ranging from about 50% to about 100%, a temperature ranging from about 10-10 C., and an air pressure ranging from about 15 kPa to about 150 kPa. The field conditioning conditions may further include a continuous, adjustable positive or negative air flow and/or and a flow of one or more continuously refreshed, selected gases. The field conditioning conditions may dehydrate the pollen to achieve a pollen moisture content of about 40% to about 58%, such as about 50% to about 57%.

Described are methods of field conditioning cereal crop pollen. A method of the present invention includes collecting fresh pollen and introducing the pollen to field conditioning conditions which regulate pollen moisture content. The field conditioning conditions may include a relative humidity ranging from about 50% to about 100%, a temperature ranging from about 10-10 C., and an air pressure ranging from about 15 kPa to about 150 kPa. The field conditioning conditions may further include a continuous, adjustable positive or negative air flow and/or and a flow of one or more continuously refreshed, selected gases. The field conditioning conditions may dehydrate the pollen to achieve a pollen moisture content of about 40% to about 58%, such as about 50% to about 57%.

Adenine derivatives substituted at the C2, N6, and N9 purine positions having antisenescent and combined photoprotective UVA/UVB effects. These substances are particularly suitable as anti-senescent and UV-photoprotective component in cosmetic preparations, plant protection preparations and in preparations for the treatment/application of tissue cultures. ##STR00001##

Adenine derivatives substituted at the C2, N6, and N9 purine positions having antisenescent and combined photoprotective UVA/UVB effects. These substances are particularly suitable as anti-senescent and UV-photoprotective component in cosmetic preparations, plant protection preparations and in preparations for the treatment/application of tissue cultures. ##STR00001##

A method for treating a perishable product including determining a desired product treatment outcome selected from sanitation, protection, preservation, or enhancement of the perishable product; directly applying a substance to the surface of the perishable product after harvesting using a carrier; wet or dry washing the perishable product; and packaging the perishable product. A system for treating a perishable product with a substance treatment including one or more devices for directed substance application to a surface of the perishable products prior to final packaging of the perishable product using a carrier; and wherein the one or more devices is an electrostatically charging device or a nebulizer. Embodiments of the invention enhance the efficacy of sanitizers and other functional substances for increasing the quality, safety, and overall shelf life of perishable products through improved coating and distribution of the sanitizers and functional substances on the surface of perishable product.

A method for treating a perishable product including determining a desired product treatment outcome selected from sanitation, protection, preservation, or enhancement of the perishable product; directly applying a substance to the surface of the perishable product after harvesting using a carrier; wet or dry washing the perishable product; and packaging the perishable product. A system for treating a perishable product with a substance treatment including one or more devices for directed substance application to a surface of the perishable products prior to final packaging of the perishable product using a carrier; and wherein the one or more devices is an electrostatically charging device or a nebulizer. Embodiments of the invention enhance the efficacy of sanitizers and other functional substances for increasing the quality, safety, and overall shelf life of perishable products through improved coating and distribution of the sanitizers and functional substances on the surface of perishable product.

Compositions comprising complexes of 1-methylcyclopropene and -cyclodextrin, and articles including the composition, are provided. Methods for using the compositions and articles in delaying the maturation of fruits, vegetables, and plants are also provided.

The present disclosure provides the use of a phosphatidylcholine compound as a component of a media composition. The resulting media composition can be used for the cryopreservation of eukaryotic cells. The cryopreserved eukaryotic cells can be thawed and recovered for inoculation and/or growth in a media to reproduce new cells. Provided herein are compositions and methods for the cryopreservation of eukaryotic cells in a media composition containing a phosphatidylcholine compound.

The present disclosure provides the use of a phosphatidylcholine compound as a component of a media composition. The resulting media composition can be used for the cryopreservation of eukaryotic cells. The cryopreserved eukaryotic cells can be thawed and recovered for inoculation and/or growth in a media to reproduce new cells. Provided herein are compositions and methods for the cryopreservation of eukaryotic cells in a media composition containing a phosphatidylcholine compound.