A reduction treatment agent made of a powder with a particle size within a range of 1500 to 3000 mesh, the powder containing: 20 to 40 parts of a mixed-oxide powder containing magnesium oxide and zinc oxide; and 60 to 80 parts of an organic acid powder containing calcium, ascorbic acid, citric acid and salt. The reduction treatment agent may further contain one to six kinds of metal powder selected from the group of copper, molybdenum, nickel, cobalt, iron and aluminum, each in an amount of one part. Due to this configuration, the reduction treatment agent can be easily mixed with various substances when added to those substances. Even if the target substance is not water, the agent can entirely and uniformly change that substance into a reduced state. Additionally, the reduction treatment agent can act as a surfactant, and therefore, can be used as cosmetics or food.

A reduction treatment agent made of a powder with a particle size within a range of 1500 to 3000 mesh, the powder containing: 20 to 40 parts of a mixed-oxide powder containing magnesium oxide and zinc oxide; and 60 to 80 parts of an organic acid powder containing calcium, ascorbic acid, citric acid and salt. The reduction treatment agent may further contain one to six kinds of metal powder selected from the group of copper, molybdenum, nickel, cobalt, iron and aluminum, each in an amount of one part. Due to this configuration, the reduction treatment agent can be easily mixed with various substances when added to those substances. Even if the target substance is not water, the agent can entirely and uniformly change that substance into a reduced state. Additionally, the reduction treatment agent can act as a surfactant, and therefore, can be used as cosmetics or food.

Methods for obtaining genetic material from plant embryos while preserving their viability are provided. In the methods, preservation of viability may be maintained by suspending the embryos in an aqueous solution surrounded by an oil matrix. Genetic material may be obtained from an aliquot of the aqueous solution and may be used directly for molecular analysis, or whole genome amplification may be performed prior to molecular analysis.

Methods for obtaining genetic material from plant embryos while preserving their viability are provided. In the methods, preservation of viability may be maintained by suspending the embryos in an aqueous solution surrounded by an oil matrix. Genetic material may be obtained from an aliquot of the aqueous solution and may be used directly for molecular analysis, or whole genome amplification may be performed prior to molecular analysis.

Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.

Described are methods of preserving pollen, which may be subsequently stored. A method of the present invention includes collecting fresh pollen and introducing the pollen to a controlled environment which regulates pollen moisture content. The controlled environment may have a temperature ranging from about −10-10° C.; an adjustable and programmable relative humidity; a continuous, adjustable positive or negative air flow; and a flow of one or more continuously refreshed, selected gases which displace oxygen. In another embodiment of the method, the controlled environment may have a relative humidity from about 50-100%; a temperature from about −10-10° C.; and air pressure from about 15-150 kPa. In all embodiments of the method, an optional field conditioning step may be performed. The field conditioning step may include subjecting the pollen to an environment controlled for relative humidity, temperature, and air pressure such that the initial pollen moisture content may be adjusted to a target moisture content.

Described are methods of preserving pollen, which may be subsequently stored. A method of the present invention includes collecting fresh pollen and introducing the pollen to a controlled environment which regulates pollen moisture content. The controlled environment may have a temperature ranging from about −10-10° C.; an adjustable and programmable relative humidity; a continuous, adjustable positive or negative air flow; and a flow of one or more continuously refreshed, selected gases which displace oxygen. In another embodiment of the method, the controlled environment may have a relative humidity from about 50-100%; a temperature from about −10-10° C.; and air pressure from about 15-150 kPa. In all embodiments of the method, an optional field conditioning step may be performed. The field conditioning step may include subjecting the pollen to an environment controlled for relative humidity, temperature, and air pressure such that the initial pollen moisture content may be adjusted to a target moisture content.

Aspects of the disclosure provide coating compositions and methods of use thereof.

Aspects of the disclosure provide coating compositions and methods of use thereof.

The present invention includes a method of preserving cannabinoid concentration in a biomass comprising: providing a hemp biomass in an aqueous solution; adding a culture of a cannabinoid preserving Bacillus bacteria to the hemp biomass solution; and culturing the hemp biomass with the Bacillus bacteria under conditions in which the cannabinoid concentration is maintained.