Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

According to the invention, it has been found that a particulate biomass containing an oxidation-sensitive material of value can be converted into a particularly easy-to-handle product in a gentle manner if it is subjected to a granulation with the addition of an agglomeration auxiliary.

The present invention is a method of wowing mushrooms, preferably but not necessarily) Basiomycetes mushrooms, on a substrate that includes unrefined, live or dried cannabis plants (whole or particulated) preferably those cannabis, strains high in CBD and low in THC or containing a preferred ratio thereof, as part or all of the growth medium for the mushrooms.

There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid particles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid particles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid particles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid particles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid particles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.

There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid particles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid particles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid particles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid particles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid particles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.

The present invention relates to a method for precisely determining protein content in edible fungus, belonging to the technical field of food detection. The method is as follows: determining total nitrogen content in an edible fungus sample by using a Kjeldahl method; additionally taking an equal amount of sample, and washing same with acid and alkali solutions to obtain chitin residues; then determining nitrogen content in the chitin residues by using the Kjeldahl method; subtracting the nitrogen content in the chitin residues from the total nitrogen content in the sample, so as to eliminate the interference of the nitrogen content in chitin; and finally obtaining precise protein content by calculation based on a formula. The present invention provides a method for precisely determining protein content in edible fungus, and provides a method for the precise assessment of the nutrient composition and value of protein in the edible fungus.

The present invention relates to a method for precisely determining protein content in edible fungus, belonging to the technical field of food detection. The method is as follows: determining total nitrogen content in an edible fungus sample by using a Kjeldahl method; additionally taking an equal amount of sample, and washing same with acid and alkali solutions to obtain chitin residues; then determining nitrogen content in the chitin residues by using the Kjeldahl method; subtracting the nitrogen content in the chitin residues from the total nitrogen content in the sample, so as to eliminate the interference of the nitrogen content in chitin; and finally obtaining precise protein content by calculation based on a formula. The present invention provides a method for precisely determining protein content in edible fungus, and provides a method for the precise assessment of the nutrient composition and value of protein in the edible fungus.

Process is described for extraction from a plant starting material, such as a fungus, for the preparation of a mixture (m) of at least one glycosaminoglycan selected from: (a) hyaluronic acid or a salt thereof (HA) having a weight average molecular weight from 10 kDa to 600 kDa; (b) chondroitin or chondroitin sulfate or a salt thereof (CS) having a weight average molecular weight comprised from 3 kDa to 50 kDa; and (c) a combination of (a) and (b).

Process is described for extraction from a plant starting material, such as a fungus, for the preparation of a mixture (m) of at least one glycosaminoglycan selected from: (a) hyaluronic acid or a salt thereof (HA) having a weight average molecular weight from 10 kDa to 600 kDa; (b) chondroitin or chondroitin sulfate or a salt thereof (CS) having a weight average molecular weight comprised from 3 kDa to 50 kDa; and (c) a combination of (a) and (b).

A preparation method of Antrodia cinnamomea water-insoluble dietary fiber. After ethanol and water extraction, Antrodia cinnamomea wastes are dried and superfine-comminuted to obtain Antrodia cinnamomea waste powder. The waste powder treated with NaOH solution containing NaBH4 is extracted it twice to obtain two extracts. The two extracts are combined and an appropriate amount of glacial acetic acid are added for neutralization. The extracts are centrifugalized and precipitate is collected. After washing the precipitate with ultrapure water, the precipitate is dissolved with LiCl-DMSO. The precipitate that is insoluble in the LiCl-DMSO solution is dialyzed and freeze-dried to obtain the water-insoluble dietary fiber component ACA-IDK of Antrodia cinnamomea. The LiCl-DMSO solution is subjected to ethanol/DMSO fractional precipitation, and then the precipitate is collected and dialyzed. The water-insoluble dietary fiber component ACA-DK of Antrodia cinnamomea is obtained after freeze-drying.