Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.

Products configured for oral use and methods for preparing such products are provided. In particular, such products may be provided in the form of a lozenge. In some embodiments, oral products described herein may include a first sugar substitute in an amount of at least about 80 weight percent, a basic amine, and an organic acid, an alkali metal salt of an organic acid, or a combination thereof. In one or more embodiments, at least a portion of the basic amine is associated with at least a portion of the organic acid or the alkali metal salt thereof, the association in the form of a basic amine-organic acid salt, an ion pair between the basic amine and a conjugate base of the organic acid, or both.

A composition enclosed in a pouch to form a pouched product configured for oral use is provided, the composition including a filler in an amount of at least 20% by weight, based on the total weight of the composition, and at least one active ingredient, at least one flavorant, or a combination thereof. The composition is loaded within the pouch at a density of about 0.45 g/cm.sup.3 or higher. Further provided are methods of preparing the pouched product.

A composition enclosed in a pouch to form a pouched product configured for oral use is provided, the composition including a filler in an amount of at least 20% by weight, based on the total weight of the composition, and at least one active ingredient, at least one flavorant, or a combination thereof. The composition is loaded within the pouch at a density of about 0.45 g/cm.sup.3 or higher. Further provided are methods of preparing the pouched product.

The disclosure provides compositions adapted for oral use and, in particular, multi-compartment oral pouched products including such compositions. Some aspects of the disclosure relate to oral pouched products including a composition adapted for oral use within a porous pouch. In particular, the porous pouch may include two or more compartments, each compartment having a content of the composition contained therein. The composition within each compartment can be different such that the composition within each compartment provides a different functional or sensory experience, such as through use of a different flavoring agent, a different active ingredient, or both a different flavoring agent and a different active ingredient.

The disclosure provides compositions adapted for oral use and, in particular, multi-compartment oral pouched products including such compositions. Some aspects of the disclosure relate to oral pouched products including a composition adapted for oral use within a porous pouch. In particular, the porous pouch may include two or more compartments, each compartment having a content of the composition contained therein. The composition within each compartment can be different such that the composition within each compartment provides a different functional or sensory experience, such as through use of a different flavoring agent, a different active ingredient, or both a different flavoring agent and a different active ingredient.

Tobacco products suitable for oral ingestion are provided. The tobacco products include a tobacco composition and at least one edible film. The edible film can include one or more of (i) a fruit or vegetable puree and (ii) a denatured protein. A multi-layer tobacco product is provided that includes a tobacco composition in the form of a compressed sheet layer and at least one edible film adjacent to the tobacco composition layer, wherein the edible film includes a puree of at least one fruit, vegetable, or a combination thereof. A process for preparing a tobacco product is also provided, such as a process that includes receiving a tobacco composition and an edible film; applying the edible film to the tobacco composition; and compressing the edible film and tobacco composition together to form a multi-layer tobacco product.

Tobacco products suitable for oral ingestion are provided. The tobacco products include a tobacco composition and at least one edible film. The edible film can include one or more of (i) a fruit or vegetable puree and (ii) a denatured protein. A multi-layer tobacco product is provided that includes a tobacco composition in the form of a compressed sheet layer and at least one edible film adjacent to the tobacco composition layer, wherein the edible film includes a puree of at least one fruit, vegetable, or a combination thereof. A process for preparing a tobacco product is also provided, such as a process that includes receiving a tobacco composition and an edible film; applying the edible film to the tobacco composition; and compressing the edible film and tobacco composition together to form a multi-layer tobacco product.

This disclosure provides tobacco plants having a mutation in PR50 and transgenic tobacco plants containing a PR50 RNAi, and methods of making and using such plants.

This disclosure provides tobacco plants having a mutation in PR50 and transgenic tobacco plants containing a PR50 RNAi, and methods of making and using such plants.