Disclosed are methods to convert nitrogen in a wastewater stream to an arginine-enriched polypeptide, which includes supplying a nitrogen-enriched wastewater stream; culturing an organism in the waste stream that produces an arginine-enriched polypeptide to produce the arginine-enriched polypeptide; and removing the produced arginine-enriched polypeptide from the wastewater stream to create a processed wastewater stream. The methods also include a method to make an arginine-enriched polypeptide enriched fertilizer by flowing the effluent from the culturing step over a cation exchange material to allow binding of the arginine enriched polypeptide to the cation exchange material; and collecting arginine-enriched polypeptide to form the arginine-enriched polypeptide enriched fertilizer. The method also includes enhancing the growth or production of a plant using the fertilizer containing the arginine-enriched polypeptide.

Disclosed are methods to convert nitrogen in a wastewater stream to an arginine-enriched polypeptide, which includes supplying a nitrogen-enriched wastewater stream; culturing an organism in the waste stream that produces an arginine-enriched polypeptide to produce the arginine-enriched polypeptide; and removing the produced arginine-enriched polypeptide from the wastewater stream to create a processed wastewater stream. The methods also include a method to make an arginine-enriched polypeptide enriched fertilizer by flowing the effluent from the culturing step over a cation exchange material to allow binding of the arginine enriched polypeptide to the cation exchange material; and collecting arginine-enriched polypeptide to form the arginine-enriched polypeptide enriched fertilizer. The method also includes enhancing the growth or production of a plant using the fertilizer containing the arginine-enriched polypeptide.

High surface area magnesium carbonate structures formed from a calcined slurry of magnesium carbonate powder and a binder and method for their use to adsorb aqueous phosphate and ammonia for recovery and repurposing as a fertilizer are disclosed. A binder is utilized to aid in the formation of useful structures. The binder significantly increase porosity and the available surface area for adsorption.

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, fertilizer, feed, biofuel manufacture, and desirably impacts, nutrient recovery from waste streams for valued byproducts production, recycle water, and alternative/renewable energy production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal biomass-processing waste or additional algae harvested as feedstock for products such as fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as fertilizer, feed, biofuels, etc. to compete with non-organic or petroleum products in the marketplace.

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, fertilizer, feed, biofuel manufacture, and desirably impacts, nutrient recovery from waste streams for valued byproducts production, recycle water, and alternative/renewable energy production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal biomass-processing waste or additional algae harvested as feedstock for products such as fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as fertilizer, feed, biofuels, etc. to compete with non-organic or petroleum products in the marketplace.

The invention provides for methods, devices, and systems for pyrolyzing biomass. A pyrolysis unit can be used for the pyrolysis of biomass to form gas, liquid, and solid products. The biomass materials can be selected such that an enhanced biochar is formed after pyrolysis. The biomass can be pyrolyzed under specified conditions such that a selected biochar core is formed. The pyrolysis process can form a stable biochar core that is inert and/or resistant to degradation. The biochar or biochar core can be functionalized to form a functionalized biochar or functionalized biochar core. Functionalization can include post-pyrolysis treatments such as supplementation with microbes or physical transformations including annealing and/or activation.

The invention provides for methods, devices, and systems for pyrolyzing biomass. A pyrolysis unit can be used for the pyrolysis of biomass to form gas, liquid, and solid products. The biomass materials can be selected such that an enhanced biochar is formed after pyrolysis. The biomass can be pyrolyzed under specified conditions such that a selected biochar core is formed. The pyrolysis process can form a stable biochar core that is inert and/or resistant to degradation. The biochar or biochar core can be functionalized to form a functionalized biochar or functionalized biochar core. Functionalization can include post-pyrolysis treatments such as supplementation with microbes or physical transformations including annealing and/or activation.

The invention provides for methods, devices, and systems for pyrolyzing biomass. A pyrolysis unit can be used for the pyrolysis of biomass to form gas, liquid, and solid products. The biomass materials can be selected such that an enhanced biochar is formed after pyrolysis. The biomass can be pyrolyzed under specified conditions such that a selected biochar core is formed. The pyrolysis process can form a stable biochar core that is inert and/or resistant to degradation. The biochar or biochar core can be functionalized to form a functionalized biochar or functionalized biochar core. Functionalization can include post-pyrolysis treatments such as supplementation with microbes or physical transformations including annealing and/or activation.

A method of producing a fertiliser composition, the method comprising: (a) providing partially decomposed organic matter; (b) contacting the organic matter with: (i) an anaerobic digestate; (ii) a source of nitrate ion; (iii) a source of ammonia; and (c) contacting the mixture obtained in step (b) with a source of carbon dioxid. Also claimed is a fertiliser composition comprising partially decomposed organic matter admixed with an anaerobic digestate, a source of nitrate ion, a source of ammonia and carbon dioxide.

A method of producing a fertiliser composition, the method comprising: (a) providing partially decomposed organic matter; (b) contacting the organic matter with: (i) an anaerobic digestate; (ii) a source of nitrate ion; (iii) a source of ammonia; and (c) contacting the mixture obtained in step (b) with a source of carbon dioxid. Also claimed is a fertiliser composition comprising partially decomposed organic matter admixed with an anaerobic digestate, a source of nitrate ion, a source of ammonia and carbon dioxide.