Provided herein is a method of generating aerosol from an aerosol-generating substrate using an aerosol-generating device with at least one heat source disposed to heat, but not burn, the aerosol-generating substrate in use. The aerosol-generating substrate has first and second portions having substantially the same composition, and/or has first and second portions and there is no physical separation between the portions. The method includes heating the aerosol-generating substrate in the aerosol-generating device, so that a temperature profile of the first portion of the aerosol-generating substrate during heating is different from a temperature profile of the second portion of the aerosol-generating substrate.

Described herein is a laminate aerosol generating material, wherein the material comprises an aerosol-forming layer attached to a carrier layer, wherein the aerosol-forming layer comprises an amorphous solid, and wherein the carrier layer has a thickness from about 10 μm to about 2.5 mm.

Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.

Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.

Described herein is a method of generating aerosol from an aerosol-generating substrate using an aerosol-generating device, the aerosol-generating device comprising at least three heating zones disposed so as to each heat a different section of the substrate to generate an aerosol without burning. The method comprises sequentially generating aerosol from each different section of substrate, wherein during heating; (i) a section of substrate is heated to an aerosol-generation temperature; (ii) another section of substrate which is heated to an intermediate temperature which is below the aerosol-generation temperature but and approximately equal to or above the minimum operating temperature; (iii) at least one of the remaining sections of substrate are heated to a minimum operating temperature which is at least sufficient to prevent condensation of volatilized components on or in the vicinity of those sections; and wherein once aerosol has been generated from a section, (a) the temperature in that section is reduced from the aerosol-generation temperature to the minimum operating temperature, (b) the section previously heated to the intermediate temperature is heated to the aerosol-generation temperature, and (c) a further section is heated to the intermediate temperature.

Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.

Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.

An aerosol generating article (10) comprises: an aerosol generating substrate (12); a mouthpiece (14); and a tipping wrapper (20) comprising: a first weakening line (22) extending around at least a portion of the tipping wrapper (20); a removable tipping wrapper portion (26) extending downstream from the first weakening line (22); an upstream tipping wrapper portion (24) extending upstream from the first weakening line (22); a first longitudinal edge (30) extending along the outer surface of the aerosol generating article; and a second longitudinal edge (32) opposed to the first longitudinal edge (30) and overlying the mouthpiece (14). The tipping wrapper (20) overlaps itself in an overlapping region (28) extending at least 45 degrees circumferentially around the aerosol generating article (10). The removable tipping wrapper portion (26) is adhered to itself by means of an area of adhesive (50) provided in the overlapping region (28) and positioned such that the removable tipping wrapper portion (26) comprises an adhesive free area (52) adjacent at least a portion of the first longitudinal edge (30).

An aerosol generating article (10) comprises: an aerosol generating substrate (12); a mouthpiece (14); and a tipping wrapper (20) comprising: a first weakening line (22) extending around at least a portion of the tipping wrapper (20); a removable tipping wrapper portion (26) extending downstream from the first weakening line (22); an upstream tipping wrapper portion (24) extending upstream from the first weakening line (22); a first longitudinal edge (30) extending along the outer surface of the aerosol generating article; and a second longitudinal edge (32) opposed to the first longitudinal edge (30) and overlying the mouthpiece (14). The tipping wrapper (20) overlaps itself in an overlapping region (28) extending at least 45 degrees circumferentially around the aerosol generating article (10). The removable tipping wrapper portion (26) is adhered to itself by means of an area of adhesive (50) provided in the overlapping region (28) and positioned such that the removable tipping wrapper portion (26) comprises an adhesive free area (52) adjacent at least a portion of the first longitudinal edge (30).

The present disclosure provides aerosol generating substrates and aerosol source members comprising aerosol generating substrates, as well as methods of manufacturing thereof. In an example implementation, an aerosol generating substrate may comprise a fibrous filler material, an aerosol forming material, and a plurality of heat conducting constituents, wherein the substrate is formed as a sheet, and wherein the heat conducting constituents are part of the sheet. The heat conducting constituents may be incorporated within the sheet, or may be formed on a surface of the sheet. In another example implementation, an aerosol source member may comprise a substrate portion formed of a collection of intermingled pieces cut from an aerosol substrate sheet. In addition, or alternatively, a substrate portion may be formed of a series of overlapping layers of an aerosol substrate sheet.