Radiolabeled anti-LAG3 antibodies and their use in immuno-PET imaging are provided herein. Included are methods of detecting the presence of LAG3 proteins in a patient or sample.

The present invention relates to a complex comprising a transition metal cation (i); a ligand (ii) comprising at least one chelating group, preferably from 1 to 4 chelating groups, more preferred 2 or 3 chelating groups, wherein the chelating group(s) is/are selected from hydroxamate group N(O)C(O)R, catechol ate group, carboxyl ate group, partly or totally protonated forms of these chelating groups and mixtures of these chelating groups and/or their partly or totally protonated forms, wherein R is hydrogen or a C1 to C5 alkyl group; and a magnetic bead (iii); wherein the magnetic bead (iii) and the ligand (ii) are covalently bonded. The invention also relates to the use of the complex for reduction of the content of at least one phosphor-oxy-substance, which preferably comprises a structural element OP(O)(O)O within its structure, in a fluid sample, as well as to a method for decreasing the content of at least one phosphor-oxy-substance, preferably a phospholipid, in a fluid sample, comprising a step of adding the complex. The invention further relates to a supernatant obtained or obtainable from this method and to the use of a supernatant obtained or obtainable from the method for qualitative and/or quantitative determination of at least one analyte in said supernatant. Furthermore, the invention relates to a method for qualitative and/or quantitative determination of at least one analyte in a fluid sample and to a method for determining the kind and/or amount of at least one phosphor-oxy-substance in a fluid sample.

The present invention relates to a complex comprising a transition metal cation (i); a ligand (ii) comprising at least one chelating group, preferably from 1 to 4 chelating groups, more preferred 2 or 3 chelating groups, wherein the chelating group(s) is/are selected from hydroxamate group N(O)C(O)R, catechol ate group, carboxyl ate group, partly or totally protonated forms of these chelating groups and mixtures of these chelating groups and/or their partly or totally protonated forms, wherein R is hydrogen or a C1 to C5 alkyl group; and a magnetic bead (iii); wherein the magnetic bead (iii) and the ligand (ii) are covalently bonded. The invention also relates to the use of the complex for reduction of the content of at least one phosphor-oxy-substance, which preferably comprises a structural element OP(O)(O)O within its structure, in a fluid sample, as well as to a method for decreasing the content of at least one phosphor-oxy-substance, preferably a phospholipid, in a fluid sample, comprising a step of adding the complex. The invention further relates to a supernatant obtained or obtainable from this method and to the use of a supernatant obtained or obtainable from the method for qualitative and/or quantitative determination of at least one analyte in said supernatant. Furthermore, the invention relates to a method for qualitative and/or quantitative determination of at least one analyte in a fluid sample and to a method for determining the kind and/or amount of at least one phosphor-oxy-substance in a fluid sample.

A composite quaternary ammonium salt cationic collector and its preparation method and uses involve: adding a halogenated alkyl group into an alkyl tertiary amine containing amido group to carry out a quaternization reaction to obtain an intermediate; and adding a catalyst into the intermediate to carry out a catalytic reaction to obtain a first mixture; adding an alkyl benzylamine into the first mixture to carry out an amidation reaction to obtain a second mixture; and desalting the second mixture to obtain a composite quaternary ammonium salt cationic collector; a molar ratio of the alkyl tertiary amine, the halogenated alkyl group, and the alkyl benzylamine is (1-1.4):(0.8-1.2):(1-1.4).

A composite quaternary ammonium salt cationic collector and its preparation method and uses involve: adding a halogenated alkyl group into an alkyl tertiary amine containing amido group to carry out a quaternization reaction to obtain an intermediate; and adding a catalyst into the intermediate to carry out a catalytic reaction to obtain a first mixture; adding an alkyl benzylamine into the first mixture to carry out an amidation reaction to obtain a second mixture; and desalting the second mixture to obtain a composite quaternary ammonium salt cationic collector; a molar ratio of the alkyl tertiary amine, the halogenated alkyl group, and the alkyl benzylamine is (1-1.4):(0.8-1.2):(1-1.4).