The present invention relates to new vitamin D compounds which are bonded to a labelling group at the C3 stereocentre by means of a linker. The present invention further relates to a method for producing these vitamin D compounds and to the use of an intermediate for producing these compounds. The present invention also relates to a method for quantitatively determining vitamin D using a vitamin D compound according to the invention as a tracer. Furthermore, the present invention relates to a reagent for determining vitamin D, containing a compound according to the invention, and to the use thereof for determining vitamin D.

The present invention relates to new vitamin D compounds which are bonded to a labelling group at the C3 stereocentre by means of a linker. The present invention further relates to a method for producing these vitamin D compounds and to the use of an intermediate for producing these compounds. The present invention also relates to a method for quantitatively determining vitamin D using a vitamin D compound according to the invention as a tracer. Furthermore, the present invention relates to a reagent for determining vitamin D, containing a compound according to the invention, and to the use thereof for determining vitamin D.

The invention relates to new intermediates in the synthesis of Eldecalcitol and to processes for the preparation of said intermediates and of Eldecalcitol.

The present invention discloses novel method for synthesizing vegan 25-OH cholesterol/Calcifediol from inexpensive crude phytosterol. According to the method, Phytosterols are reacted to form corresponding i-steroid through tosylation and methanolysis. i-steroid on reductive ozonolysis to C-22 alcohol and conversion via C-22 tosylate to C-22 iodide in good yield. Coupling of C-22 tosylate with Grignard reagent of 4-bromo-2-methyl-2-[(trimethylsilyl)oxy]butane followed by deprotection yielded 25-OH cholesterol. In a process variant, nickel mediated conjugate addition of C-22 iodide to an electron deficient alkene ethyl acrylate and treating corresponding ester with methyl magnesium bromide as means of installing the side chain of 25-OH cholesterol in high yield. Further bromination reaction of 25-OH cholesterol diacetate followed by dehydrobromination using TBAF yielded 25-OH 7-dehydrocholesterol. Further photo reaction of 25-OH 7-dehydrocholesterol in to previtamin D3 using high or medium pressure mercury lamp and subsequent thermal reaction of previtamin D3 to 25-OH vitamin D3 (Calcifediol) in good yield.

The present invention provides methods, devices, and compositions to rapidly detect analytes, including small analytes, using a lateral flow device. Described herein is such a lateral flow device that can detect and quantify vitamin D in a whole blood, serum, or plasma sample by employing a sandwich-based immunoassay.

The present invention is intended to provide a compound useful to analyze a vitamin D compound. The present invention provides an alkyne compound represented by Formula (I):

##STR00001##

where in Formula (I), A is a linear carbon chain having an alkynyl group at an end and having a vinyl group at another end; X.sub.1 is CH or .sup.13CH; X.sub.2 is CHY, CDY, .sup.13CHY, .sup.13CDY, CO, .sup.13CO, C.sup.18O, or .sup.13C.sup.18O; m is an integer from 1 to 4; when m is 2 or more, X.sub.2s are identical or different; Y is H, D, NH.sub.2, .sup.15NH.sub.2, OH, .sup.18OH, SH, OR, O(CO)R, OSO.sub.3H, OSO.sub.3Na, or a sugar substituent; when Formula (I) contains two or more Ys, Ys are identical or different; R is an alcohol protective group, an alkyl group, an alkenyl group, or an aryl group; X.sub.3 is C or .sup.13C; and at least one selected from the group consisting of X.sub.1, X.sub.2(s), and X.sub.3 is modified with a stable isotope D, .sup.13C, .sup.18O, or .sup.15N.

The present invention is intended to provide a compound useful to analyze a vitamin D compound. The present invention provides an alkyne compound represented by Formula (I):

##STR00001##

where in Formula (I), A is a linear carbon chain having an alkynyl group at an end and having a vinyl group at another end; X.sub.1 is CH or .sup.13CH; X.sub.2 is CHY, CDY, .sup.13CHY, .sup.13CDY, CO, .sup.13CO, C.sup.18O, or .sup.13C.sup.18O; m is an integer from 1 to 4; when m is 2 or more, X.sub.2s are identical or different; Y is H, D, NH.sub.2, .sup.15NH.sub.2, OH, .sup.18OH, SH, OR, O(CO)R, OSO.sub.3H, OSO.sub.3Na, or a sugar substituent; when Formula (I) contains two or more Ys, Ys are identical or different; R is an alcohol protective group, an alkyl group, an alkenyl group, or an aryl group; X.sub.3 is C or .sup.13C; and at least one selected from the group consisting of X.sub.1, X.sub.2(s), and X.sub.3 is modified with a stable isotope D, .sup.13C, .sup.18O, or .sup.15N.

Novel biaromatic compounds, which are vitamin D analogs, processes for their preparation and cosmetic, dermatological and pharmaceutical preparations containing one or more of these compounds.

Novel biaromatic compounds, which are vitamin D analogs, processes for their preparation and cosmetic, dermatological and pharmaceutical preparations containing one or more of these compounds.

A reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein and an in vitro method for the detection of a vitamin D compound in which the vitamin D compound is released from vitamin D-binding protein by the use of this reagent composition as well as the reagent mixture obtained in this manner. Also disclosed is the use of the reagent compositions to release vitamin D compounds as well as a kit for detecting a vitamin D compound.