The present invention provides a method for the preparation of intermediates useful in the synthesis of gemcitabine-[phenyl-benzoxy-L-alaninyl)]-phosphate. It also provides a method of preparing gemcitabine-[phenyl-benzoxy-L-alaninyl)]-phosphate.

The present disclosure discloses a method for preparation of derivatives of gram-positive bacteria surface capsular polysaccharide, and belongs to the field of carbohydrate chemistry. The present disclosure takes glucose as a glycosyl donor to obtain a target β-glucosidic bond, then successfully synthesizes a disaccharide building block through a method of redox of a glucose C-2 site, and then takes the disaccharide building block as a repeat unit to synthesize a target oligosaccharide structure such as a derivative [.fwdarw.3)-α-D-Manp-(1.fwdarw.4)-β-D-Rhap-(1.fwdarw.].sub.5-Linker of gram-positive bacteria cell wall capsular polysaccharide. A reduction end of decose is linked with a linker to be linked with a protein to make glycoconjugates for immunological studies. The method provided by the present disclosure is simple, time-saving, labor-saving and low-cost, and the resultant derivatives of the gram-positive bacteria surface capsular polysaccharide may be used for development and preparation of medicine related to autism.

The present disclosure discloses a method for preparation of derivatives of gram-positive bacteria surface capsular polysaccharide, and belongs to the field of carbohydrate chemistry. The present disclosure takes glucose as a glycosyl donor to obtain a target β-glucosidic bond, then successfully synthesizes a disaccharide building block through a method of redox of a glucose C-2 site, and then takes the disaccharide building block as a repeat unit to synthesize a target oligosaccharide structure such as a derivative [.fwdarw.3)-α-D-Manp-(1.fwdarw.4)-β-D-Rhap-(1.fwdarw.].sub.5-Linker of gram-positive bacteria cell wall capsular polysaccharide. A reduction end of decose is linked with a linker to be linked with a protein to make glycoconjugates for immunological studies. The method provided by the present disclosure is simple, time-saving, labor-saving and low-cost, and the resultant derivatives of the gram-positive bacteria surface capsular polysaccharide may be used for development and preparation of medicine related to autism.

The present disclosure relates to a preparation method for a D-psicose crystal containing 98% (w/w) or more D-psicose and 0.05% (w/w) or less ethanol based on 100% (w/w) of the entire crystal. The preparation method includes a first step of mixing a D-psicose-containing solution and an organic solvent, and a second step of adding a seed to the mixed solution according to the first step and then cooling the same to obtain a massecuite containing the D-psicose crystal. Therefore, it is possible to increase the yield of the D-psicose crystal from the D-psicose crystal solution and prepare a D-psicose crystal of sufficient size and appropriate shape for use in mass production with no bad taste/smell.

The present disclosure relates to a preparation method for a D-psicose crystal containing 98% (w/w) or more D-psicose and 0.05% (w/w) or less ethanol based on 100% (w/w) of the entire crystal. The preparation method includes a first step of mixing a D-psicose-containing solution and an organic solvent, and a second step of adding a seed to the mixed solution according to the first step and then cooling the same to obtain a massecuite containing the D-psicose crystal. Therefore, it is possible to increase the yield of the D-psicose crystal from the D-psicose crystal solution and prepare a D-psicose crystal of sufficient size and appropriate shape for use in mass production with no bad taste/smell.

Proposed are allulose concentrates in solid amorphous form, which are characterized in that they contain at least approximately 20 wt. % allulose, and a method for producing said concentrates.

The present disclosure provides for antibody-oligonucleotide conjugates, methods of preparation thereof, and methods of use thereof. Also provided are related compounds, compositions and kits.

The present invention relates to methods and intermediates for the synthesis of nucleosides and nucleoside analogues (NAs). More specifically, the present invention relates to methods of synthesizing nucleosides and NAs, using simple achiral materials by a ‘one-pot’ proline-catalyzed halogenation of a heteroaryl-substituted acetaldehyde together with a tandem enantioselective aldol reaction followed by a reduction or organometallic addition and cyclization (annulation) reaction involving halide displacement.

The present invention relates to methods and intermediates for the synthesis of nucleosides and nucleoside analogues (NAs). More specifically, the present invention relates to methods of synthesizing nucleosides and NAs, using simple achiral materials by a ‘one-pot’ proline-catalyzed halogenation of a heteroaryl-substituted acetaldehyde together with a tandem enantioselective aldol reaction followed by a reduction or organometallic addition and cyclization (annulation) reaction involving halide displacement.

Methods of carbohydrate acetylation are disclosed. A method may include adding a carbohydrate to a reaction vessel, adding poly-4-vinylpyriding (P4VP) to the reaction vessel, adding a bio-derived solvent to the reaction vessel, adding acetic anhydride (Ac20) to the reaction vessel, and adding a catalyst to the reaction vessel. The bio-derived solvent may be 2-methyltetrahydrofuran (2-MeTHF). A catalyst may also be added to the reaction vessel.