A synthetic hydrogel is described, including hydrated mucin glycoproteins cross-linked with multi-arm thiol functional cross-linker, which can be prepared to model viscoelastic and micro-rheological properties of natural mucus. Such synthetic hydrogel can be prepared from a wide variety of mucin raw materials. Also described is a method of microrheologically characterizing mucus, by dispersing in the mucus muco-inert particles (MIP), irradiating the mucus containing MIP with polarized light, and measuring fluorescence polarization (FP) resulting from rotational diffusion of the MIP in the mucus in response to such irradiating, as a microrheological characteristic of the mucus. This method can be carried out using a plate reader equipped with a spectrofluorometer and polarized filter set, and therefore can be readily carried out in clinical settings without the necessity of specialized microrheological equipment.

The present disclosure provides an epoxidized natural rubber composite and a preparation process thereof, and relates to the technical field of rubber materials. The epoxidized natural rubber composite provided by the present disclosure comprises the following preparation raw materials in parts by weight: 100 parts of epoxidized natural rubber, 130 parts of peanut meal, 0.050.8 parts of surfactants, 0.14 parts of coagulant and 0.212 parts of vulcanization processing aids. The present disclosure utilizes essential amino acids and non-essential amino acids contained in peanut meal to improve the aging resistance of the epoxidized natural rubber. After hot air aging, ozone aging and ultraviolet aging treatments, both the tensile strength retention rate and the elongation at break retention rate of the epoxidized natural rubber composites of the present disclosure can be kept above 83%.

The present disclosure provides an epoxidized natural rubber composite and a preparation process thereof, and relates to the technical field of rubber materials. The epoxidized natural rubber composite provided by the present disclosure comprises the following preparation raw materials in parts by weight: 100 parts of epoxidized natural rubber, 130 parts of peanut meal, 0.050.8 parts of surfactants, 0.14 parts of coagulant and 0.212 parts of vulcanization processing aids. The present disclosure utilizes essential amino acids and non-essential amino acids contained in peanut meal to improve the aging resistance of the epoxidized natural rubber. After hot air aging, ozone aging and ultraviolet aging treatments, both the tensile strength retention rate and the elongation at break retention rate of the epoxidized natural rubber composites of the present disclosure can be kept above 83%.

The present invention forms a detection layer in an embedded biosensor probe by using a phenazine derivative as a redox mediator in which a phenazine group is covalently bonded to a high molecular weight polymer having a carboxyl group or an amino group, such as polyamino acid, polyimine, or polyallylamine; and the distance between the phenazine group and the high molecular weight polymer main chain is increased by using a polyethylene glycol chain.

The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.

A protein-binding product comprising one or more porous polymer beads, wherein at least one ligand is bound to the surfaces of said polymer beads via a spacer (R), and wherein said at least one ligand is Gal1-3HexNAcO, Gal1-4GlcNAcO and/or a derivative thereof, is disclosed as well as a device containing said protein-binding product and a method for reduction of the level of at least one galactose-binding protein and optionally at least one other protein in human blood plasma.

Hydrogels comprising a macromolecular matrix and water may be used to augment soft tissue of a human being, promote or support cell or tissue viability or proliferation, create space in tissue, and for other purposes. A macromolecular matrix may comprise a hyaluronic acid component crosslinked to a silk fibroin component.

Hydrogels comprising a macromolecular matrix and water may be used to augment soft tissue of a human being, promote or support cell or tissue viability or proliferation, create space in tissue, and for other purposes. A macromolecular matrix may comprise a hyaluronic acid component crosslinked to a silk fibroin component.

Materials and methods for generating protein-polymer conjugates on solid supports are provided herein. The methods can include, for example, reversibly immobilizing a protein on a solid support, modifying the protein by adding polymer subunits, and releasing the protein-polymer conjugate from the solid support.

Compositions useful as dermal fillers and methods using such compositions to treat various skin and soft tissue conditions. The dermal fillers can comprise silk attached to hyaluronic acid using for example two cross linkers and can be used to treat of facial imperfections, facial defects, facial augmentations, breast imperfections, breast augmentations or breast reconstructions.