A method of crosslinking a protein or peptide for use as a biomaterial, the method comprising the step of irradiating a photoactivatable metal-ligand complex and an electron acceptor in the presence of the protein or peptide, thereby initiating a cross-linking reaction to form a 3-dimensional matrix of the biomaterial.

The present invention relates to a method for preparing a protein cage which comprises: a 1.sup.st step of preparing an amphiphilic polymer comprising a 1.sup.st hydrophobic polymer and a 1.sup.st hydrophilic functional group; a 2.sup.nd step of preparing a hydrophilic protein comprising a 2.sup.nd functional group binding to the 1.sup.st functional group; a 3.sup.rd step of forming an amphiphilic polymer-protein hybrid by the binding of the 1.sup.st functional group and the 2.sup.nd functional group, and forming core-shell structured particles comprising a protein shell and an amphiphilic polymer core by the self-assembly of the amphiphilic polymer in a hydrophilic solvent; and a fourth step of removing some or all of the hydrophobic polymer of the core part from the core-shell structured particles.

The present invention relates to a method for preparing a protein cage which comprises: a 1.sup.st step of preparing an amphiphilic polymer comprising a 1.sup.st hydrophobic polymer and a 1.sup.st hydrophilic functional group; a 2.sup.nd step of preparing a hydrophilic protein comprising a 2.sup.nd functional group binding to the 1.sup.st functional group; a 3.sup.rd step of forming an amphiphilic polymer-protein hybrid by the binding of the 1.sup.st functional group and the 2.sup.nd functional group, and forming core-shell structured particles comprising a protein shell and an amphiphilic polymer core by the self-assembly of the amphiphilic polymer in a hydrophilic solvent; and a fourth step of removing some or all of the hydrophobic polymer of the core part from the core-shell structured particles.

A bifunctional polymer is functionalized at one end with an azlactone end group to conjugate biomolecules of interest, and is functionalized at another end with an azide anchor group to attach the polymer to a substrate. Methods of making the bifunctional polymer are also provided. A coated substrate includes the bifunctionalized polymers on the surface of a substrate. Methods of making the coated substrate are also provided. A microarray includes a plurality of discrete regions, each region including the coated substrate.

A bifunctional polymer is functionalized at one end with an azlactone end group to conjugate biomolecules of interest, and is functionalized at another end with an azide anchor group to attach the polymer to a substrate. Methods of making the bifunctional polymer are also provided. A coated substrate includes the bifunctionalized polymers on the surface of a substrate. Methods of making the coated substrate are also provided. A microarray includes a plurality of discrete regions, each region including the coated substrate.

A biodegradable core-shell microcapsule composition with controlled release of an active material is provided, wherein the shell of the microcapsule is composed of a biopolymer cross-linked with a combination of two or more different types of cross-linking agents.

Disclosed are collagen nanoparticles obtained from waste scaly skins from fish by using a micro fluid technique, a method for obtaining the collagen nanoparticles, and their field of use.

A composition includes a target pharmaceutical or biological agent, a solution containing the target pharmaceutical or biological agent, and substrate that is soluble in the solution. The substrate is capable of being solidified via a solidification process and the solidification process causes the substrate to become physically or chemically cross-linked, vitrified, or crystallized. As a result of the solidification process, particles are formed. The target pharmaceutical or biological agent within the solution retains proper conformation to ultimately produce a desired effect.

A composition includes a target pharmaceutical or biological agent, a solution containing the target pharmaceutical or biological agent, and substrate that is soluble in the solution. The substrate is capable of being solidified via a solidification process and the solidification process causes the substrate to become physically or chemically cross-linked, vitrified, or crystallized. As a result of the solidification process, particles are formed. The target pharmaceutical or biological agent within the solution retains proper conformation to ultimately produce a desired effect.

In some embodiments, the present disclosure provides methods including the steps of (i) providing silk fibroin material comprising substantially amorphous structure, and (ii) applying at least one of elevated temperature and elevated pressure to the silk fibroin material to form a silk fibroin article, wherein the applying induces fusion between at least a portion of the silk fibroin and structural change of fibroin in the silk fibroin material. In some embodiments, the present disclosure also provides silk fibroin articles made in accordance with the methods disclosed herein.