The present invention provides a solvent-free viscoelastic RSF liquid by modifying the RSF surface with polyethylenimine and polyethylene glycol-based polymer surfactant, which surrounds each RSF molecule by forming a dual shell and thereby minimizes the inter RSF interactions and thus prevents formation of β-sheet aggregation. The engineering of RSF surface with PEI and PS significantly improved the conformational stability and storage time of RSF liquid compared to native RSF as silk I conformation of RSF liquid remained intact for more than 8 months.

The present disclosure relates to a self-assembled nanostructure of an elastin- and resilin-based block copolypeptide with stimuli responsiveness and resilience for drug delivery, tissue engineering and regenerative medicine, a method for preparing the same and application thereof. The diblock polypeptide reversibly forms a self-assembled micelle structure in response to temperature stimuli and a hydrogel prepared using the triblock polypeptide undergoes reversible sol-gel or gel-sol transition in response to temperature stimuli and exhibits enhanced mechanical strength due to chemical crosslinkages between tyrosine residues. With such superior properties, the diblock/triblock polypeptide of the present disclosure can be used for drug delivery systems, scaffolds for tissue engineering and kits for tissue or organ regeneration.

Provided is a method of forming a fibrin hydrogel composition, the method including providing a fibrin hydrogel or precursor thereof, comprising fibrin hydrogel forming salt. The fibrin hydrogel forming salt concentration is greater than or equal to the threshold concentration to form a fibrin hydrogel. The method further includes combining the fibrin hydrogel with a carrier material. The concentration of the carrier material typically ranges from 0.1 to about 50 wt.-%. The method further includes reducing the salt concentration below the threshold concentration to form a fibrin hydrogel.

We have developed novel shear-thinning biomaterials using silica nanoparticles, gelatin-based polymers and small molecules such as doxorubicin. Shear-thinning biomaterial technology offers enables polymers and drugs loaded inside such polymers to be easily delivered directly through catheters into target area for use, for example, in cancer therapy and immunotherapy. When a force above a certain threshold is applied to inject such materials, they “thin” and behaves as a semi-solid, allowing the material to readily flow through a catheter. When the force is removed, the material instantly becomes a soft solid with significant cohesive properties that prevent it from dislodging or breaking up.

Provided are methods for linking polypeptides (including peptides and proteins) to other moieties using radical imitated thiol-ene chemistries, for example, modifying a polypeptide by introducing reactive thiol groups and reacting the thiol groups with olefin-containing reagents or alkyne-containing reagents under conditions that support radical thiol-ene or thiol-yne reactions. The reactive thiol groups have greater activity for radical thiol-ene reactions that a cysteine thiol group, including thiol groups that are separated from the peptide backbone by at least two carbon atoms, for example, the thiol group of a homocysteine residue. Also provided are compositions and biomaterials containing the linked polypeptides, for example, peptide and protein conjugates, and thiol-ene based biocompatible hydrogel polymers, and their uses in the medical field.

The present invention relates to a method of making a porous sponge-like formulation that can well absorb water, oil and organic solvents separately or combined. Methods of preparing said formulation and its use in medical, pharmaceutical, biotechnological, chemical as well as in wound care, home care, (agro-)environmental and construction material applications are also provided.

The present disclosure relates to a method of preparing polymeric microparticles that can realize excellent mechanical strength and stability as well as high crosslinking efficiency and production yield, polymeric microparticles, medical compositions, cosmetic compositions, medical articles and cosmetic articles comprising the same.

A method for producing a natural formaldehyde-free adhesive for wood panels, which improves the crosslinking reactions between the crosslinking agent and the binding agent, comprising reacting a plant protein selected from soy, lupine and legumes, together with a crosslinking agent selected from 100% of an oxidized saccharide or a combination of an oxidized saccharide and an unoxidized or pure saccharide; the method comprises the steps of: an oxidation step of a saccharide; a preparation step of plant protein, in which the plant protein is reacted with a chaotropic agent or alkali or by temperature control or a combination of these conditions; and a mixing step.

Problem to be solved: To provide a whitened or opacified capsule and film without using a white pigment such as titanium dioxide. Solution: A film, a capsule and a film-forming composition characterized by containing a film-forming polymer component selected from gelatin and pullulan and an opacifying agent consisting of a water-soluble salt except calcium salt carbonate and bicarbonate, wherein the salt is selected from a sodium salt, potassium salt, an ammonium salt and a magnesium salt.

A process for manufacturing a tubular film such as an edible casing film or a packaging film. The process includes the steps of providing a preblended powder composition containing a polymer matrix, a plasticizer, and water; feeding the preblended powder composition to an extruder; heating the preblended powder composition to a temperature above 100 degrees Celsius for a sufficient time to fully hydrate the polymer matrix and to convert the powder composition to a flowable mass; and extruding the flowable mass through a tubular die of the extruder to form the tubular film. The tubular film comprises: about 40-75 wt % polymer matrix; about 10-35 wt % plasticizer; and about 10-35 wt % water. The polymer matrix component is fully hydrated under the temperature, pressure and shear conditions inside the extruder, and may have a component which is only fully hydrated at temperatures above about 100 degrees Celsius.