This invention relates generally to water-insoluble but water-swellable and deformable crosslinked PEGylated microgel particles of proteins and protein-based macromolecules that are pseudoplastic (shear thinning) and flow in aqueous media under shear and which can be injected or made to flow, wherein said microgel particles can reform as a cluster of microgel particles when shearing forces are removed. The microgel particles function as a matrix to support cell growth, viability, and proliferation.

It is an object of the present invention to provide a cellulose-containing composition having excellent coating suitability, a production method thereof, and a film. According to the present invention, provided is a cellulose-containing composition comprising cellulose fibers having a fiber width of 1000 nm or less and protein, wherein the protein includes an enzyme, the content of the protein is 1×10.sup.−3 parts by mass or less with respect to 1 part by mass of the cellulose fibers, and when the cellulose-containing composition having a solid concentration of 0.4% by mass is obtained, the viscosity of the cellulose-containing composition measured under conditions of 25° C. and a rotation number of 3 rpm is 10 mPa.Math.s or more and 11000 mPa.Math.s or less.

It is an object of the present invention to provide a cellulose-containing composition having excellent coating suitability, a production method thereof, and a film. According to the present invention, provided is a cellulose-containing composition comprising cellulose fibers having a fiber width of 1000 nm or less and protein, wherein the protein includes an enzyme, the content of the protein is 1×10.sup.−3 parts by mass or less with respect to 1 part by mass of the cellulose fibers, and when the cellulose-containing composition having a solid concentration of 0.4% by mass is obtained, the viscosity of the cellulose-containing composition measured under conditions of 25° C. and a rotation number of 3 rpm is 10 mPa.Math.s or more and 11000 mPa.Math.s or less.

Described herein are fluid complex coacervates that produce solid adhesives in situ. Oppositely charged polyelectrolytes were designed to form fluid adhesive complex coacervates at ionic strengths higher than the ionic strength of the application site, but an insoluble adhesive solid or gel at the application site. When the fluid, high ionic strength adhesive complex coacervates are introduced into the lower ionic strength application site, the fluid complex coacervate is converted to a an adhesive solid or gel as the salt concentration in the complex coacervate equilibrates to the application site salt concentration. In one embodiment, the fluid complex coacervates are designed to solidify in situ at physiological ionic strength and have numerous medical applications. In other aspects, the fluid complex coacervates can be used in aqueous environment for non-medical applications.

Described herein are fluid complex coacervates that produce solid adhesives in situ. Oppositely charged polyelectrolytes were designed to form fluid adhesive complex coacervates at ionic strengths higher than the ionic strength of the application site, but an insoluble adhesive solid or gel at the application site. When the fluid, high ionic strength adhesive complex coacervates are introduced into the lower ionic strength application site, the fluid complex coacervate is converted to a an adhesive solid or gel as the salt concentration in the complex coacervate equilibrates to the application site salt concentration. In one embodiment, the fluid complex coacervates are designed to solidify in situ at physiological ionic strength and have numerous medical applications. In other aspects, the fluid complex coacervates can be used in aqueous environment for non-medical applications.

Biocompatible phase invertible proteinaceous compositions and methods for making and using the same are provided. Phase invertible compositions in accordance with the invention are prepared by combining a liquid proteinaceous substrate and a liquid crosslinking composition, where the liquid crosslinking composition includes a macromolecular crosslinking agent. Also provided are kits for use in preparing the subject compositions. The subject compositions, kits and systems find use in a variety of different applications.

Biocompatible phase invertible proteinaceous compositions and methods for making and using the same are provided. Phase invertible compositions in accordance with the invention are prepared by combining a liquid proteinaceous substrate and a liquid crosslinking composition, where the liquid crosslinking composition includes a macromolecular crosslinking agent. Also provided are kits for use in preparing the subject compositions. The subject compositions, kits and systems find use in a variety of different applications.

Disclosed herein are medical products, including an implantable device coated with a crosslinked extracellular matrix comprising at least one of Type IV collagen and laminin, wherein the crosslinked extracellular matrix contains no more than 0.024 mg/ml total concentration of glucose, amino acids and salts having a molecular weight of 2000 daltons or less. Corresponding systems and method also are disclosed.

Disclosed herein are medical products, including an implantable device coated with a crosslinked extracellular matrix comprising at least one of Type IV collagen and laminin, wherein the crosslinked extracellular matrix contains no more than 0.024 mg/ml total concentration of glucose, amino acids and salts having a molecular weight of 2000 daltons or less. Corresponding systems and method also are disclosed.

Compositions and methods related to hydrogel tissue sealants are generally described. In certain embodiments, a hydrogel forming composition is provided in dry form (e.g., as one or more powder mixtures) and comprises at least an electrophilic polymer crosslinking agent and a nucleophilic polymer such as a protein that is capable of crosslinking with the crosslinking agent. One or more solvents able to dissolve the crosslinking agent and the protein can be provided and used to dissolve the hydrogel forming composition to facilitate crosslinking.