The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

The present invention provides a carboxylate-bridged binuclear iron-sulfur clusters fluorescent probe having the structure of Formula I. The preparation method comprises the following steps: anthracenylmethanamine and p-methoxycarbonylphenyl isocyanate have an addition reaction to get a substitutional methyl benzoate, which is hydrolyzed to get a corresponding carboxylic acid; react the resulting carboxylic acid with alkali to get carboxylate, and then coordinate with binuclear iron precursor to obtain the fluorescent probe. Compared with the prior art, the invention firstly provides the carboxylate-bridged binuclear iron-sulfur clusters of metal complex which similar to the central structure of bio-enzyme. The metal complex, as a fluorescent probe, has good selectivity to the fluorinion detection. The fluorescence titration experiment is simple and easy to operate and the fluorescence changes are sensitive.

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The present application relates to a substituted benzanthracene compound of a formula (I) or (II). The application furthermore relates to an electronic device which comprises the said benzanthracene compound.

An aromatic amine derivative is represented by the following formula (1). In the formula (1), R.sub.1 to R.sub.10 each independently represent a hydrogen atom and a substituent. In the formula (1); R.sub.1 is represented by the following formula (2); any one of R.sub.2 to R.sub.5 and R.sub.7 to R.sub.10 is represented by the following formula (2); and L.sub.1 to L.sub.3 each independently represent a single bond, a divalent residue of an aryl group, or the like. In the formula (2); Ar.sub.1 is a monovalent substituent having a partial structure represented by the following formula (3); X represents an oxygen atom or a sulfur atom; and A and B represent a six-membered ring. In the formula (2), Ar.sub.2 is an aryl group, a monovalent substituent having a partial structure represented by the formula (3), and the like. ##STR00001##

An aromatic amine derivative is represented by the following formula (1). In the formula (1), R.sub.1 to R.sub.10 each independently represent a hydrogen atom and a substituent. In the formula (1); R.sub.1 is represented by the following formula (2); any one of R.sub.2 to R.sub.5 and R.sub.7 to R.sub.10 is represented by the following formula (2); and L.sub.1 to L.sub.3 each independently represent a single bond, a divalent residue of an aryl group, or the like. In the formula (2); Ar.sub.1 is a monovalent substituent having a partial structure represented by the following formula (3); X represents an oxygen atom or a sulfur atom; and A and B represent a six-membered ring. In the formula (2), Ar.sub.2 is an aryl group, a monovalent substituent having a partial structure represented by the formula (3), and the like. ##STR00001##

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

A fused aromatic ring derivative shown by the following formula (1): ##STR00001##
wherein R.sub.a and R.sub.b are independently a hydrogen atom or a substituent, p is an integer of 1 to 8 and q is an integer of 1 to 11, when p is 2 or more, plural R.sub.as may be independently the same or different and adjacent R.sub.as may form a ring, when q is 2 or more, plural R.sub.bs may be independently the same or different, L.sub.1 is a single bond, or a substituted or unsubstituted divalent linking group, and Ar.sub.1 is a substituted or unsubstituted aryl group having 6 to 50 ring carbon atoms or a substituted or unsubstituted heteroaryl group having 5 to 50 ring atoms, provided that the substituent of L.sub.1, the substituent of Ar.sub.1, R.sub.a and R.sub.b contain no substituted or unsubstituted amino group.

A fused aromatic ring derivative shown by the following formula (1): ##STR00001##
wherein R.sub.a and R.sub.b are independently a hydrogen atom or a substituent, p is an integer of 1 to 8 and q is an integer of 1 to 11, when p is 2 or more, plural R.sub.as may be independently the same or different and adjacent R.sub.as may form a ring, when q is 2 or more, plural R.sub.bs may be independently the same or different, L.sub.1 is a single bond, or a substituted or unsubstituted divalent linking group, and Ar.sub.1 is a substituted or unsubstituted aryl group having 6 to 50 ring carbon atoms or a substituted or unsubstituted heteroaryl group having 5 to 50 ring atoms, provided that the substituent of L.sub.1, the substituent of Ar.sub.1, R.sub.a and R.sub.b contain no substituted or unsubstituted amino group.

An organic electroluminescence element that uses a compound expressed by the following general formula has low inter-molecular interaction and high orientation during vapor deposition, and by using compounds that are resistant to aggregation, luminous efficiency is high, and there is little change in chromaticity accompanying drive deterioration (either V.sup.1 or W.sup.1 and either V.sup.2 or W.sup.2 form rings, the rings formed by V.sup.1 and V.sup.2 are six-membered rings, and the rings formed by W.sup.1 and W.sup.2 are five-membered rings; A.sup.2 to A.sup.4 and A.sup.6 to A.sup.8 represent CR.sup.Z or N, and R.sup.Z represents a hydrogen atom or a substituent; and R.sup.2, R.sup.3, R.sup.7, and R.sup.8 represent a hydrogen atom, an alkyl group, an aryl group, a heteroaryl group, a fluorine atom, a hydrogen atom [sic], or a deuterium atom.). ##STR00001##

An organic electroluminescence element that uses a compound expressed by the following general formula has low inter-molecular interaction and high orientation during vapor deposition, and by using compounds that are resistant to aggregation, luminous efficiency is high, and there is little change in chromaticity accompanying drive deterioration (either V.sup.1 or W.sup.1 and either V.sup.2 or W.sup.2 form rings, the rings formed by V.sup.1 and V.sup.2 are six-membered rings, and the rings formed by W.sup.1 and W.sup.2 are five-membered rings; A.sup.2 to A.sup.4 and A.sup.6 to A.sup.8 represent CR.sup.Z or N, and R.sup.Z represents a hydrogen atom or a substituent; and R.sup.2, R.sup.3, R.sup.7, and R.sup.8 represent a hydrogen atom, an alkyl group, an aryl group, a heteroaryl group, a fluorine atom, a hydrogen atom [sic], or a deuterium atom.). ##STR00001##