The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I):

##STR00001##

In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.

According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I):

##STR00001##

In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.

A compound having a tricyclic nucleus and having formula (I)

##STR00001##

wherein Ar is C.sub.3-C.sub.25 aryl in which at least one aromatic ring atom is nitrogen; X is O, S or CR.sup.9R.sup.10; R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6 independently are hydrogen, deuterium, C.sub.1-C.sub.12 alkyl, C.sub.4-C.sub.12 aryl or C.sub.2-C.sub.12 alkenyl; R.sup.7 and R.sup.8 independently are hydrogen, deuterium, C.sub.1-C.sub.12 alkyl, C.sub.4-C.sub.12 aryl or C.sub.2-C.sub.12 alkenyl or R.sup.7 and R.sup.8 groups attached to a nitrogen atom are joined by a single bond, O, S or CR.sup.11R.sup.12 to form a single nitrogen-containing substituent; R.sup.9 and R.sup.10 independently are hydrogen, deuterium, C.sub.1-C.sub.12 alkyl, C.sub.4-C.sub.12 aryl or C.sub.2-C.sub.12 alkenyl; and R.sup.11 and R.sup.12 independently are hydrogen, deuterium, C.sub.1-C.sub.12 alkyl, C.sub.4-C.sub.12 aryl or C.sub.2-C.sub.12 alkenyl; provided that Ar does not contain an aromatic ring attached to the tricyclic nucleus which contains more than two aromatic ring nitrogen atoms.

The present invention relates to organic light emitting elements, comprising thermally activated delayed fluorescence (TADF) emitters and/or hosts on basis of benzotriazoles, which have a sufficiently small energy gap between S.sub.1 and T.sub.1 (?E.sub.ST) to enable up-conversion of the triplet exciton from T.sub.1 to S.sub.1. The organic light emitting elements show high electroluminescent efficiency.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

The presently-disclosed subject matter includes azetidine-substituted fluorescent compounds, where the compounds may be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

Sulfonated 2(7)-aminoacridone and 1-aminopyrene dyes and their use as fluorescent tags, in particular for carbohydrate analysis The invention relates to fluorescent dyes with multiple negatively charged groups in their ionized form which are aminoacridone sulfonamides or 1-aminopyrenes having of one of the following general formulae A-D: Formula (A), Formula (B), Formula (C), Formula (D), wherein the ionizable groups X are typically selected from the following: SH, COOH, SO.sub.3H, OSO.sub.3H, OP(O)(OH).sub.2, OP(O)(OH)R.sup.a, P(O)(OH).sub.2, P(O)(OH)R.sup.a, where R.sup.aC.sub.1-C.sub.4alkyl or substituted C.sub.1-C.sub.4alkyl. The invention further relates to the use of these dyes as fluorescent tags, in particular for reducing sugars and glycans. ##STR00001##

Sulfonated 2(7)-aminoacridone and 1-aminopyrene dyes and their use as fluorescent tags, in particular for carbohydrate analysis The invention relates to fluorescent dyes with multiple negatively charged groups in their ionized form which are aminoacridone sulfonamides or 1-aminopyrenes having of one of the following general formulae A-D: Formula (A), Formula (B), Formula (C), Formula (D), wherein the ionizable groups X are typically selected from the following: SH, COOH, SO.sub.3H, OSO.sub.3H, OP(O)(OH).sub.2, OP(O)(OH)R.sup.a, P(O)(OH).sub.2, P(O)(OH)R.sup.a, where R.sup.aC.sub.1-C.sub.4alkyl or substituted C.sub.1-C.sub.4alkyl. The invention further relates to the use of these dyes as fluorescent tags, in particular for reducing sugars and glycans. ##STR00001##