Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.

Disclosed are liquid and solid assay compositions and portable sample reader devices for use in a sample-to-answer diagnostic system for the detection of one or more analytes, preferably the detection of circulating histones in whole blood. Further provided are methods of making and using the assay compositions and portable sample reader, including the collection of a raw sample, testing the sample using the assay compositions, and analyzing the sample via the portable sample reader. More particularly, assay compositions comprising a sacrificial partition, target molecule, detectable label, and sacrificial partition are used in combination with a sample reader comprising an optical system and a housing unit as part of a sample-to-answer diagnostic system for quantifying circulating histones in whole blood as a mechanism of predicting the risk of multiple organ failure.

An article that includes a fluorescent composition having at least one of a fluorescent sensor compound and organic reporter molecules encapsulated in a microsphere structure. When encapsulated, the fluorescent sensor compound and the organic reporter molecules are distributed in a liquid organic matrix. When non-encapsulated, the remaining one of the fluorescent sensor compound and the organic reporter molecules reside in the matrix. In response to a force applied to the composition sufficient to break at least a portion of the microsphere structure, the fluorescent sensor compound and the organic reporter molecules are transformed into a non-reversible fluorescent state exhibiting a quantum yield greater than 0.2. The fluorescent state is objectively visually verifiable without physically contacting the composition.

Provided are a ruthenium complex dye having a water content of 0.2% to 4.0% by mass; a dye solution including the ruthenium complex dye; a photoelectric conversion element having semiconductor fine particles having the ruthenium complex dye carried thereon; and a dye-sensitized solar cell including the photoelectric conversion element.

A compound represented by Chemical Formula 1, and a photoelectric device, an image sensor, and an electronic device including the same are disclosed.

##STR00001##

In Chemical Formula 1, each substituent is the same as defined in the detailed description.

The present disclosure provides fluorogenic compounds useful for preparing fluorescent calcium ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators in methods of detection, discrimination and quantification of metal ions. The subject fluorogenic compounds and fluorescent ion indicators can include a chelating group based on a 2-aminophenoxyethylene glycol 2-aminoethyl ether, N,N,N′,N′-tetraacetic acid (PEGTA) moiety or precursor thereof where the phenyl group of the PEGTA is substituted with or fused with a fluorophore moiety of interest. The subject methods find use in the detection of intracellular calcium ions. Also provided are kits for use in practicing the subject methods.

According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I): ##STR00001##
In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.

According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I): ##STR00001##
In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.

A 4-hydroxquinoline compound has the formula (I)

##STR00001## wherein A is selected from diradicals of the formulae (A.1), (A.2), (A.3), (A.4), (A.5), and (A.6) wherein R.sup.1, R.sup.2a, R.sup.2b, R.sup.3, if present R.sup.4a, R.sup.4b, R.sup.5a, R.sup.5b, R.sup.6a, R.sup.6b, R.sup.6c, R.sup.6d, R.sup.n1, R.sup.n2, R.sup.n3, R.sup.n4, R.sup.m5, R.sup.m6, R.sup.m7, R.sup.m8, R.sup.7, R.sup.8a, R.sup.9 are as defined in the description.

A 4-hydroxquinoline compound has the formula (I)

##STR00001## wherein A is selected from diradicals of the formulae (A.1), (A.2), (A.3), (A.4), (A.5), and (A.6) wherein R.sup.1, R.sup.2a, R.sup.2b, R.sup.3, if present R.sup.4a, R.sup.4b, R.sup.5a, R.sup.5b, R.sup.6a, R.sup.6b, R.sup.6c, R.sup.6d, R.sup.n1, R.sup.n2, R.sup.n3, R.sup.n4, R.sup.m5, R.sup.m6, R.sup.m7, R.sup.m8, R.sup.7, R.sup.8a, R.sup.9 are as defined in the description.