A method for coating a surface of a cell culture article includes dissolving a polymer having a covalently attached polypeptide in an aqueous solution to produce a polymer solution. The polymer is formed from monomers selected to form a polymer having a linear backbone, wherein the polymer is crosslink free. The weight percentage of the polypeptide relative to the polymer conjugated to the polypeptide is sufficiently high to render the polymer conjugated to the polypeptide water soluble. The aqueous solution is substantially free of organic solvents. The method further includes (i) disposing the polymer solution on the surface of the cell culture article to produce a coated article; and (ii) subjecting the coated article to sufficient heat or electromagnetic radiation to attach the polymer conjugated to a polypeptide to the surface of the cell culture article.

Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.

Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.

The invention provides a silk fibroin-based anti-counterfeiting mark comprising a silk fibroin film layer and a gold film layer located on the silk fibroin film layer, and the area of the gold film layer is less than that of the silk fibroin film layer. The inventive also provides a method for preparing the silk fibroin-based optical anti-counterfeiting mark, the method comprises preparing an aqueous silk fibroin solution, coating the aqueous silk fibroin solution onto a substrate to obtain a silk fibroin film, and sputtering a layer of gold film on the silk fibroin film with a mask to obtain the silk fibroin-based optical anti-counterfeiting mark. In the invention a thin layer of Au film is sputtered on a silk fibroin film such that an anti-counterfeiting mark pattern appears at a high humidity, the method is simple and can significantly the production cost of optical anti-counterfeiting mark.

The invention provides a silk fibroin-based anti-counterfeiting mark comprising a silk fibroin film layer and a gold film layer located on the silk fibroin film layer, and the area of the gold film layer is less than that of the silk fibroin film layer. The inventive also provides a method for preparing the silk fibroin-based optical anti-counterfeiting mark, the method comprises preparing an aqueous silk fibroin solution, coating the aqueous silk fibroin solution onto a substrate to obtain a silk fibroin film, and sputtering a layer of gold film on the silk fibroin film with a mask to obtain the silk fibroin-based optical anti-counterfeiting mark. In the invention a thin layer of Au film is sputtered on a silk fibroin film such that an anti-counterfeiting mark pattern appears at a high humidity, the method is simple and can significantly the production cost of optical anti-counterfeiting mark.

Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

The present invention describes tunable methods of treating cellulosic materials with a barrier coating comprising a prolamine and at least one polyol fatty acid ester that provides increased oil and/or grease resistance to such materials without sacrificing the biodegradability thereof. The methods as disclosed provide for adhering of the barrier coating on articles including articles comprising cellulosic materials and articles made by such methods. The materials thus treated display higher lipophobicity and may be used in any application where such features are desired.

The present invention describes tunable methods of treating cellulosic materials with a barrier coating comprising a prolamine and at least one polyol fatty acid ester that provides increased oil and/or grease resistance to such materials without sacrificing the biodegradability thereof. The methods as disclosed provide for adhering of the barrier coating on articles including articles comprising cellulosic materials and articles made by such methods. The materials thus treated display higher lipophobicity and may be used in any application where such features are desired.

A coating, a method for coating surfaces, and the coated surfaces. The method includes providing a substrate with a cleaned metal surface; contacting and coating the metal surface with an aqueous composition having a ph of from 0.5 to 7.0 and in the form of a dispersion and/or a suspension; optionally rinsing the organic coating; and drying and/or baking the organic coating, or optionally drying the organic coating and coating same with a similar or another coating composition thereto. The composition contains a complex fluoride in a quantity of 1.1 10.sup.6 mol/l to 0.30 mol/l based on the cations. An anionic polyelectrolyte in a quantity of 0.01 to 5.0 wt % based on the total mass of the resulting mixture is added to an anionically stabilized dispersion made of film-forming polymers and/or a suspension made of film-forming inorganic particles.