Provided herein are processes for preparing fluorescent 1-cyano-2-substituted isoindole compounds or N-substituted phthalazinium compounds, comprising reacting an aromatic dialdehyde or aromatic aldehyde-ketone compound with a material that contains primary amino or hydrazine groups, and assaying methods involving the processes thereof.

Provided are a phosphor-containing capable of suppressing deterioration of phosphors and can be manufactured with high efficiency and a backlight unit. Specifically, provided is a phosphor-containing film 1, including a first substrate film 10; and a phosphor-containing layer 30 at which a plurality of regions 35 containing phosphors 31, which, if exposed to oxygen, deteriorate by reacting with the oxygen, are discretely disposed on the first substrate film 10, and at which a resin layer 38 having an impermeability to oxygen is disposed between the discretely disposed regions 35 containing phosphors 31, in which a width S of the resin layer 38 between the regions 35 containing phosphors 31 is 0.01S<0.5 mm, and wherein a ratio of a volume Vp of the regions containing phosphors, to a sum of the volume Vp and a volume Vb of the resin layer in the phosphor-containing layer, is 0.1Vp/(Vp+Vb)<0.9.

Provided are a phosphor-containing capable of suppressing deterioration of phosphors and can be manufactured with high efficiency and a backlight unit. Specifically, provided is a phosphor-containing film 1, including a first substrate film 10; and a phosphor-containing layer 30 at which a plurality of regions 35 containing phosphors 31, which, if exposed to oxygen, deteriorate by reacting with the oxygen, are discretely disposed on the first substrate film 10, and at which a resin layer 38 having an impermeability to oxygen is disposed between the discretely disposed regions 35 containing phosphors 31, in which a width S of the resin layer 38 between the regions 35 containing phosphors 31 is 0.01S<0.5 mm, and wherein a ratio of a volume Vp of the regions containing phosphors, to a sum of the volume Vp and a volume Vb of the resin layer in the phosphor-containing layer, is 0.1Vp/(Vp+Vb)<0.9.

Provided are a scintillator with improved energy sensitivity dependence within the energy range of diagnostic X-rays, more specifically in the range of 40-150 kV, and a radiation dosimeter using same. Due to the scintillator comprising a photopolymer resin that contains a polymerizable monomer, a filler, and a photopolymerization initiator, energy sensitivity dependence within the range of 40-150 kV is improved. Furthermore, changes in relative sensitivity within this energy range can be reduced to 3% or less by containing an inorganic fluorescent substance such as Zn.sub.2SiO.sub.4.

Provided are a scintillator with improved energy sensitivity dependence within the energy range of diagnostic X-rays, more specifically in the range of 40-150 kV, and a radiation dosimeter using same. Due to the scintillator comprising a photopolymer resin that contains a polymerizable monomer, a filler, and a photopolymerization initiator, energy sensitivity dependence within the range of 40-150 kV is improved. Furthermore, changes in relative sensitivity within this energy range can be reduced to 3% or less by containing an inorganic fluorescent substance such as Zn.sub.2SiO.sub.4.

To suppress a decrease in optical output of a scintillator. A scintillator includes a sintered body of 1 mm.sup.3 or less that contains a rare earth oxysulfide. In a composition image obtained by observing a cross-section of the sintered body under a scanning electron microscope, the sum of the number of oxide regions that contain at least one of a rare earth oxide different from the rare earth oxysulfide and an impurity metal oxide and the number of sulfide regions that contain at least one of a rare earth sulfide different from the rare earth oxysulfide and an impurity metal sulfide, which exist in a unit area of 500 m500 m, is zero or more and five or less. Each of the oxide regions and the sulfide regions has a major axis of zero or more and 100 m or less.

A multi-compartment microcapsule emits photons when subjected to a stimulus. In some embodiments, the multi-compartment microcapsules have first and second compartments separated by an isolating structure adapted to rupture in response to the stimulus, wherein the first and second compartments contain reactants that come in contact and react to produce photons when the isolating structure ruptures.

A multi-compartment microcapsule emits photons when subjected to a stimulus. In some embodiments, the multi-compartment microcapsules have first and second compartments separated by an isolating structure adapted to rupture in response to the stimulus, wherein the first and second compartments contain reactants that come in contact and react to produce photons when the isolating structure ruptures.

This fluorescence observation method is a method of observing a living organism into which a fluorescent dye is injected. The method includes the steps of: irradiating the living organism with excitation light including a wavelength for exciting the fluorescent dye using a light irradiation means, acquiring a first fluorescence image of the living organism generated by the irradiation with the excitation light using an image acquisition means, specifying an observation object in the living organism on the basis of the first fluorescence image; acquiring a second fluorescence image of the observation object generated by the irradiation with the excitation light using the image acquisition means; and specifying a linear fluorescence pattern appearing in the second fluorescence image.

This fluorescence observation method is a method of observing a living organism into which a fluorescent dye is injected. The method includes the steps of: irradiating the living organism with excitation light including a wavelength for exciting the fluorescent dye using a light irradiation means, acquiring a first fluorescence image of the living organism generated by the irradiation with the excitation light using an image acquisition means, specifying an observation object in the living organism on the basis of the first fluorescence image; acquiring a second fluorescence image of the observation object generated by the irradiation with the excitation light using the image acquisition means; and specifying a linear fluorescence pattern appearing in the second fluorescence image.