A biofuel production method that does not require a pH adjustment of a biomass raw material as a pretreatment is provided. A biofuel production method according to the present disclosure includes mixing a terrestrial-derived raw material, i.e., a terrestrial biomass, with a marine-derived raw material, i.e., a marine biomass, and performing fermentation using the mixed raw material, thereby producing ethanol.

A biofuel production method that does not require a pH adjustment of a biomass raw material as a pretreatment is provided. A biofuel production method according to the present disclosure includes mixing a terrestrial-derived raw material, i.e., a terrestrial biomass, with a marine-derived raw material, i.e., a marine biomass, and performing fermentation using the mixed raw material, thereby producing ethanol.