A system includes an algae bioreactor that contains an algae slurry, a heat exchanger in fluid communication with the algae bioreactor to receive the algae slurry from the algae bioreactor and heat and increase a pressure of the algae slurry, and one or more valves and a flash vessel in fluid communication with a discharge of the heat exchanger to flash the algae slurry and create steam and algae biomass. A separator receives the algae biomass from the flash vessel and separates oils from the algae biomass to generate a biofuel.

The present invention relates to process for purifying renewable feedstock comprising at least one acidulated soap-stock, wherein said process comprises the steps, where the renewable feedstock comprising at least one fatty acid is treated in a treating step with an aqueous medium, and a first stream comprising water and a second stream comprising fatty acids are obtained, and the second stream is obtained as purified renewable feedstock.

The present invention relates to process for purifying renewable feedstock comprising at least one acidulated soap-stock, wherein said process comprises the steps, where the renewable feedstock comprising at least one fatty acid is treated in a treating step with an aqueous medium, and a first stream comprising water and a second stream comprising fatty acids are obtained, and the second stream is obtained as purified renewable feedstock.

A novel method is developed to remove wax and terpene from the wild hemp plants to produce enriched cannabidiol (CBD) crude oil with high quality and further purify the crude oil into crystal/isolate with 99.0% content of CBD. The method utilizes titanium dioxide (TiO.sub.2) photocatalyst for redox reaction under the irradiation of UV (ultraviolet) light in a solvent extraction process of CBD, which significantly removes accompanying wax and terpene. With the beneficial photocatalyst property, the TiO.sub.2 decomposes the wax and terpene in the wild hemp crude extract through appropriate UV radiation to induce catalytic reaction at specific PH (potential hydrogen) levels. Through optimizing the photocatalyst dewaxing using TiO.sub.2, the method achieves the best dewaxing effect and maximum terpene reduction. Thus, the method offers a low-cost, reusable, and biologically friendly process of removing wax and terpene to maximize the efficiency and effectiveness of the separation processes in the CBD production.

An improved process for producing refined oils free of contaminating waxes, resins, and lipids from botanical plant matter using an improved in-line winterization process is disclosed. The biomass is fed into an extraction vessel where the oils are extracted using a solvent, followed by a two-stage separation. Both the temperature and pressure of the solvent going into the separators are controlled for effective separation. Additional solvent is added to enhance the separation efficiency between the refined oil and the undesired fraction containing the contaminants. This process provides an alternative to current wax mitigation techniques, such as alcohol based solvent winterization.

The present invention relates generally to corn dry-milling, and more specifically, to a method and system for removing insoluble solids mid-evaporation in a corn (or similar carbohydrate-containing grain) dry milling process for making alcohol, such as ethanol, and/or other biofuels/biochemicals. In one example, the method for removing residual insoluble solids in a grain dry milling process includes separating a whole stillage byproduct into an insoluble solids portion and a solubles portion, which includes residual insoluble solids. Then, the solubles portion is subjected to a first evaporation, via one or more evaporators, to remove liquid from the solubles portion to define a concentrated solubles portion. After the first evaporation, the residual insoluble solids are separated from the concentrated solubles portion. And thereafter, the concentrated solubles portion is subjected to a second evaporation, via one or more evaporators, to remove additional liquid from the concentrated solubles portion.

Systems and methods to reduce pour point (PP) temperatures of fat-based compositions for use in transportation fuels. In one or more embodiments, methods and systems reduce the pour point of rendered fats using biologically-derived plant oils for effectively transporting the blended fat based compositions over long distances, thereby advantageously decreasing the heating and mixing requirements needed to maintain the compositional temperature above the pour point. In certain embodiments, the fat based composition comprises rendered animal fats, such as tallow in combination with distilled corn oil (DCO).

Systems and methods to reduce pour point (PP) temperatures of fat-based compositions for use in transportation fuels. In one or more embodiments, methods and systems reduce the pour point of rendered fats using biologically-derived plant oils for effectively transporting the blended fat based compositions over long distances, thereby advantageously decreasing the heating and mixing requirements needed to maintain the compositional temperature above the pour point. In certain embodiments, the fat based composition comprises rendered animal fats, such as tallow in combination with distilled corn oil (DCO).

The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present disclosure relates methods and systems for refining grain oil compositions using water, and related compositions produced therefrom. The present disclosure also relates to methods of using grain oil derived from a fermentation product in an anti-foam composition and/or feedstock to make diesel.