Methods for producing oil from distillers corn oil having high free fatty acid content are provided. In the method, distiller's corn oil is treated with a mixture including an alcohol to result in a low-free fatty acid oily phase and an alcohol phase. The mixture may also include an alkali. The alcohol may be a monohydric alcohol and an aqueous alcohol, such as an aqueous alcohol having a concentration of at least about 15% alcohol-by-weight. The low-free fatty acid phase may include oil and at least one impurity. The low-free fatty acid phase may be cooled, and the oil may be separated from the at least one impurity using membrane filtration.

Methods for producing oil from distillers corn oil having high free fatty acid content are provided. In the method, distiller's corn oil is treated with a mixture including an alcohol to result in a low-free fatty acid oily phase and an alcohol phase. The mixture may also include an alkali. The alcohol may be a monohydric alcohol and an aqueous alcohol, such as an aqueous alcohol having a concentration of at least about 15% alcohol-by-weight. The low-free fatty acid phase may include oil and at least one impurity. The low-free fatty acid phase may be cooled, and the oil may be separated from the at least one impurity using membrane filtration.

Present invention relates to a novel process for purification of lipid material for further use as such as e.g. a source of fuel or chemicals.

Provided is a mutant of the wild type phosphatidylcholine-specific phospholipase C of Bacillus cereus. The mutations involved comprise the amino acid residue at position 63 being mutated from asparagine to aspartic acid, the amino acid residue at position 131 being mutated from asparagine to serine, and the amino acid residue at position 134 being mutated from asparagine to aspartic acid, and may comprise the amino acid residue at position 56 being mutated from tyrosine to alanine, lysine, asparagine, glutamine, histidine or tryptophan, and further, may also comprise the amino acid residue at position 106 being mutated from methionine to valine. Also provided are a polynucleotide molecule encoding the mutant, a nucleic acid construct and a host cell comprising the polynucleotide molecule, a composition comprising the mutant, and the use of the mutant, the polynucleotide molecule, the nucleic acid construct and the host cell.

Provided is a mutant of the wild type phosphatidylcholine-specific phospholipase C of Bacillus cereus. The mutations involved comprise the amino acid residue at position 63 being mutated from asparagine to aspartic acid, the amino acid residue at position 131 being mutated from asparagine to serine, and the amino acid residue at position 134 being mutated from asparagine to aspartic acid, and may comprise the amino acid residue at position 56 being mutated from tyrosine to alanine, lysine, asparagine, glutamine, histidine or tryptophan, and further, may also comprise the amino acid residue at position 106 being mutated from methionine to valine. Also provided are a polynucleotide molecule encoding the mutant, a nucleic acid construct and a host cell comprising the polynucleotide molecule, a composition comprising the mutant, and the use of the mutant, the polynucleotide molecule, the nucleic acid construct and the host cell.

The present invention provides a composition comprising 10-40 mass % of C.sub.8-30 linear alkanes, up to 20 mass % of C.sub.7-20 aromatic hydrocarbons, at least 90 mass % of which are monoaromatic, and no more than 1 mass % in total of oxygen-containing compounds; wherein the total amount of C.sub.8-30 alkanes in the composition is 50-95 mass %, and the total amount of C.sub.8-30 alkanes, C.sub.7-20 aromatic hydrocarbons and C.sub.8-30 cycloalkanes is at least 95 mass %; wherein the composition comprises 45-90 mass % in total of C.sub.8-30 cycloalkanes and C.sub.8-30 branched alkanes; and wherein the amounts are based on the mass of the composition. Also provided is a method of producing the composition comprising the step of hydroprocessing a biological feedstock using a catalyst and the step of fractionating the product of the hydroprocessing step.

The present invention provides a composition comprising 10-40 mass % of C.sub.8-30 linear alkanes, up to 20 mass % of C.sub.7-20 aromatic hydrocarbons, at least 90 mass % of which are monoaromatic, and no more than 1 mass % in total of oxygen-containing compounds; wherein the total amount of C.sub.8-30 alkanes in the composition is 50-95 mass %, and the total amount of C.sub.8-30 alkanes, C.sub.7-20 aromatic hydrocarbons and C.sub.8-30 cycloalkanes is at least 95 mass %; wherein the composition comprises 45-90 mass % in total of C.sub.8-30 cycloalkanes and C.sub.8-30 branched alkanes; and wherein the amounts are based on the mass of the composition. Also provided is a method of producing the composition comprising the step of hydroprocessing a biological feedstock using a catalyst and the step of fractionating the product of the hydroprocessing step.

The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.

Described herein is a polymerized biorenewable, petroleum based, previously modified, or functionalized oil, comprising a polymeric distribution ranging from about 2 to about 80 wt % oligomer content, a hydroxyl value ranging from about 0 to about 400, and an iodine value ranging from about 0 to about 200. Methods of manufacturing the polymerized oil as well as its incorporation into asphalt paving, roofing, and coating applications are also described.

Described herein is a polymerized biorenewable, petroleum based, previously modified, or functionalized oil, comprising a polymeric distribution ranging from about 2 to about 80 wt % oligomer content, a hydroxyl value ranging from about 0 to about 400, and an iodine value ranging from about 0 to about 200. Methods of manufacturing the polymerized oil as well as its incorporation into asphalt paving, roofing, and coating applications are also described.