The present application provides mutants of wild type phospholipase C (PLC) specific to phosphatidylcholine from Bacillus cereus. The related mutations include mutation of asparagine at position 63 to another amino acid, also including mutation of arginine at position 20 to histidine and of alanine at position 83 to aspartic acid. The present application also provides a nucleic acid molecule encoding the mutant, a vector containing the nucleic acid molecule, and a cell containing the nucleic acid molecule or vector. The present application also provides uses of the mutant, nucleic acid molecule vector, and cell.

A system and method for degumming oil is used to increase oil yield and reduce impurities such as phosphorus. An oil feedstock, water and enzyme mixture is passed through a high shear mixing device prior to being fed to an agitated reactor equipped with a recirculation system. The recirculation system includes a second high shear mixing device that further mixes the oil mixture in the reactor during processing. The reacted mixture is discharged from the reactor to a downstream post-reaction system for separating the degummed oil from the reacted mixture. The separation step can be used to remove the phosphatides and other impurities from the reacted oil to form a purified oil product.

Methods to prepare vegetable oil compositions having an elevated ethyl ester content are provided.

A method for lowering saturated fatty acid content, the method comprising concentrating polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to react with a glyceride containing a polyunsaturated fatty acid; wherein the lipase reaction is performed at a temperature of not more than 25? C.

A method of synthesizing silver nanoparticles includes using sesame (Sesamum indicum) oil cake extract as a reducing agent. The silver nanoparticles can range in size from about 6 nm to about 15 nm. The silver nanoparticles can have an average particle size of about 10 nm.

Processes are described for refining a renewable oil. The processes may include the steps of adding one or more compounds to the renewable oil to produce a soap stock in the renewable oil, and separating at least a portion of the soap stock from the renewable oil. The processes may further include adding a polymer to the separated renewable oil to produce a refined renewable oil.

This invention discloses new krill oil compositions characterized by having high amounts of phospholipids, astaxanthin esters and/or omega-3 contents. The krill oils are obtained from krill meal using supercritical fluid extraction in a two stage process. Stage 1 removes the neutral lipid by extracting with neat supercritical CO.sub.2 or CO.sub.2 plus approximately 5% of a co-solvent. Stage 2 extracts the actual krill oils by using supercritical CO.sub.2 in combination with approximately 20% ethanol. The krill oil materials obtained are compared with commercially available krill oil and found to be more bioeffective in a number of areas such as anti-inflammation, anti-oxidant effects, improving insulin resistances and improving blood lipid profile.

The present invention relates to a process for degumming a vegetable oil, comprising a. contacting an oil-water mixture comprising a crude vegetable oil comprising phospholipids with an enzyme having a phospholipase activity, wherein the oil-water mixture comprises an aqueous solution having a molal ionic strength of between 0.001 and 0.5 mol/kg; b. separating an oil-water mixture into an oil composition and an aqueous composition; and, c. washing the oil composition with an acid, wherein a degummed vegetable is produced.

An electrical device containing an enzymatically-degummed vegetable oil is disclosed. Also disclosed are methods for insulating and cooling a transformer using enzymatically-degummed vegetable oils, and methods for adding an enzymatically-degummed vegetable oil to an enclosure of an electrical device. Further disclosed are processes for making dielectric fluids using enzyme-degumming of vegetable oils or using enzyme-degummed vegetable oils as the starting material for the process.

The present invention relates to processes for recovering/extracting oil from fermentation product production processes based on starch-containing material, wherein an alpha-amylase, a high dosage of protease, and optionally a glucoamylase, are present and/or added in liquefaction. The invention also relates to processes for producing fermentation products and to enzyme compositions suitable for use in processes of the invention.