The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

A method for reducing neutral oil losses during a neutralization step including (1) mixing an acid-treated vegetable oil with a base to neutralize (a) free fatty acid and (b) added acid in the acid-treated vegetable oil to obtain a pretreated mixture; (2) subjecting the pretreated mixture to at least two successive cycles of localized shear effects, each cycle including a step of forcing the pretreated mixture at a pressure through one or more nozzles, each cycle of localized shear effects having a shear duration time of less than 510.sup.5 seconds and a shear rate of at least about 6000 sec.sup.1.

Provided herein are methods of optimizing energy recovery from oilseeds. The methods disclosed provide at least the ability to swell oilseeds and disrupt the cell walls (hulls) without changing the functionality and quality of oil; the process integration of removing the impurities from oilseeds, oil extraction, protein hydrolysis, and green coal production to maximize the energy recovery in the form of crude oil, polypeptides, oligopeptides, amino acids, and/or green coal from oilseeds; and heat integration during processing stages including subcritical water pretreatment, oil extraction, subcritical water post-treatment, and subcritical water carbonization to minimize the process heat requirement.

This invention discloses new krill oil compositions characterized by having high amounts of phospholipids, astaxanthin esters and/or omega-3 contents. The krill oils are obtained from krill meal using supercritical fluid extraction in a two stage process. Stage 1 removes the neutral lipid by extracting with neat supercritical CO.sub.2 or CO.sub.2 plus approximately 5% of a co-solvent. Stage 2 extracts the actual krill oils by using supercritical CO.sub.2 in combination with approximately 20% ethanol. The krill oil materials obtained are compared with commercially available krill oil and found to be more bioeffective in a number of areas such as anti-inflammation, anti-oxidant effects, improving insulin resistances and improving blood lipid profile.

A method of separating oil from a composition containing an oil and water emulsion, by adding a separation additive which is a fatty ester of alkoxylated glycerol, and performing at least one oil separation step. The method is particularly suitable for separating corn oil from stillage produced in a corn ethanol mill.

Methods are provided for refining natural oil feedstocks. The methods comprise reacting the feedstock in the presence of a metathesis catalyst under conditions sufficient to form a metathesized product comprising olefins and esters. In certain embodiments, the methods further comprise separating the olefins from the esters in the metathesized product. In certain embodiments, the methods further comprise hydrogenating the olefins under conditions sufficient to form a fuel composition. In certain embodiments, the methods further comprise transesterifying the esters in the presence of an alcohol to form a transesterified product.

Methods are provided for refining natural oil feedstocks. The methods comprise reacting the feedstock in the presence of a metathesis catalyst under conditions sufficient to form a metathesized product comprising olefins and esters. In certain embodiments, the methods further comprise separating the olefins from the esters in the metathesized product. In certain embodiments, the methods further comprise hydrogenating the olefins under conditions sufficient to form a fuel composition. In certain embodiments, the methods further comprise transesterifying the esters in the presence of an alcohol to form a transesterified product.

The present invention relates to Coix seed oil extracted from Semen Coicis, pharmaceutical preparations thereof, and the use thereof in the treatment of tumors. Specifically, the Coix seed oil contains 11 triglyceride ingredients in the following mass percentages: trilinolein 4.87-6.99%, 1-olein-2,3-dilinolein 13.00-18.69%, 1-palmitin-2,3-dilinolein 5.25-7.54%, 1,3-diolein-2-linolein 13.23-19.02%, 1-palmitin-2-linolein-3-olein 10.26-14.75%, 1,3-dipalmitin-2-linolein 2.28-3.28%, triolein 14.44-20.76% and 1-palmitin-2,3-diolein 8.06-11.58%, 1-olein-2-linolein-3-stearin 1.37-1.97%, 1,3-dipalmitin-2-olein 1.52-2.19% and 1,2-diolein-3-stearin 1.29-1.86%.

Extracting lipids from a feedstock includes contacting a feedstock including water, lipids, and solid organic matter with an organic solvent to yield a mixture; separating the mixture to yield a solid and a liquid; and desolventizing the solid to yield a solid product and a fluid product. The organic solvent may include a polar organic solvent, a non-polar organic solvent, or a combination thereof. The lipids in the mixture are in the liquid state. The solid product has a lipids content of 20 wt % or less. In some cases, the number-weighted particle size distribution of the solid organic matter has a mean dimension of 1 mm or less, the lipids content of the feedstock is at least 20 wt %, the water content of the feedstock is 10 wt % or less or 5 wt % or less, or a combination thereof.

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture which is a fish oil or which is derived from fish oil, which process comprises the steps of: (i) purifying the feed mixture in a chromatographic separation step, to obtain a first intermediate product; and (ii) purifying the first intermediate product obtained in (i) in a simulated or actual moving bed chromatographic separation step, to obtain a second intermediate product; and (iii) purifying the second intermediate product obtained in (ii) in a simulated or actual moving bed chromatographic separation step, to obtain the PUFA product; wherein an aqueous organic solvent is used as eluent in each separation step; saturated and/or monounsaturated fatty acids present in the feed mixture are removed in the first separation step; the PUFA product is separated from different components of the feed mixture in steps (ii) and (iii); and the PUFA product obtained in the third separation step contains EPA or an EPA derivative in an amount greater than 90 wt %.