The present invention relates to methods of producing industrial products from plant lipids, particularly from vegetative parts of plants. In particular, the present invention provides oil products such as biodiesel and synthetic diesel and processes for producing these, as well as plants having an increased level of one or more non-polar lipids such as triacylglycerols and an increased total non-polar lipid content. In one particular embodiment, the present invention relates to combinations of modifications in two or more of lipid handling enzymes, oil body proteins, decreased lipid catabolic enzymes and/or transcription factors regulating lipid biosynthesis to increase the level of one or more non-polar lipids and/or the total non-polar lipid content and/or mono-unsaturated fatty acid content in plants or any part thereof. In an embodiment, the present invention relates to a process for extracting lipids. In another embodiment, the lipid is converted to one or more hydrocarbon products in harvested plant vegetative parts to produce alkyl esters of the fatty acids which are suitable for use as a renewable biodiesel fuel.

The present invention relates to methods of producing industrial products from plant lipids, particularly from vegetative parts of plants. In particular, the present invention provides oil products such as biodiesel and synthetic diesel and processes for producing these, as well as plants having an increased level of one or more non-polar lipids such as triacylglycerols and an increased total non-polar lipid content. In one particular embodiment, the present invention relates to combinations of modifications in two or more of lipid handling enzymes, oil body proteins, decreased lipid catabolic enzymes and/or transcription factors regulating lipid biosynthesis to increase the level of one or more non-polar lipids and/or the total non-polar lipid content and/or mono-unsaturated fatty acid content in plants or any part thereof. In an embodiment, the present invention relates to a process for extracting lipids. In another embodiment, the lipid is converted to one or more hydrocarbon products in harvested plant vegetative parts to produce alkyl esters of the fatty acids which are suitable for use as a renewable biodiesel fuel.

Methods for producing oil from distillers corn oil having high free fatty acid content are provided. In the method, distiller's corn oil is treated with a mixture including an alcohol to result in a low-free fatty acid oily phase and an alcohol phase. The mixture may also include an alkali. The alcohol may be a monohydric alcohol and an aqueous alcohol, such as an aqueous alcohol having a concentration of at least about 15% alcohol-by-weight. The low-free fatty acid phase may include oil and at least one impurity. The low-free fatty acid phase may be cooled, and the oil may be separated from the at least one impurity using membrane filtration.

The present invention relates to lipid comprising docosapentaenoic acid and/or docosahexaenoic acid preferentially esterified at the sn-2 position of triacylglycerol, and processes for producing and using the lipid.

A process for dry fractionation of a soft palm oil mid fraction (SPMF) into a final hard palm oil mid fraction (fHPMF-A) is disclosed. The process comprises: providing the solf palm oil mid fraction (SPMF), using the soft palm oil mid fraction (SPMF) as an input (IN1) to a first dry fractionation (FDF) to obtain an intermediate olein fraction (SPMF-O) and an intermediate stearin fraction (SPMF-S), using the intermediate olein fraction (SPMF-O) as an input (IN2) to an ultrasound assisted second dry fractionation (SDF) to obtain the final hard palm oil mid fraction (fHPMF-A) and a palm oil olein fraction (POO), wherein the ultrasound assisted second dry fractionation (SPF) comprises subjecting at least a part of the input (IN2) to ultrasonic treatment (US2). Also disclosed is a final hard palm oil mid fraction (fHPMF-A), a second hard palm oil mid fraction (sHPMF-B), a hard palm oil mid fraction mixture, and uses of these.

A process for dry fractionation of a soft palm oil mid fraction (SPMF) into a final hard palm oil mid fraction (fHPMF-A) is disclosed. The process comprises: providing the solf palm oil mid fraction (SPMF), using the soft palm oil mid fraction (SPMF) as an input (IN1) to a first dry fractionation (FDF) to obtain an intermediate olein fraction (SPMF-O) and an intermediate stearin fraction (SPMF-S), using the intermediate olein fraction (SPMF-O) as an input (IN2) to an ultrasound assisted second dry fractionation (SDF) to obtain the final hard palm oil mid fraction (fHPMF-A) and a palm oil olein fraction (POO), wherein the ultrasound assisted second dry fractionation (SPF) comprises subjecting at least a part of the input (IN2) to ultrasonic treatment (US2). Also disclosed is a final hard palm oil mid fraction (fHPMF-A), a second hard palm oil mid fraction (sHPMF-B), a hard palm oil mid fraction mixture, and uses of these.

Provided herein are systems and methods for the facile extraction and purification of oils from plant material, including cannabis and hemp. The systems and methods herein are versatile, and may utilize a wide range of solvents to extract oils from a variety of plant-based material. Further, the provided systems and methods are closed loop, reducing the loss solvent and decreasing the risk of safety concerns such as human exposure to solvent chemicals or explosion of volatiles. In some embodiments, the systems and methods remove impurities from the extracted oils, for example waxes or other precipitates, and provide a higher purity and higher quality extract.

Triglyceride oils having one or more populations of asymmetric triglyceride molecules are provided. Asymmetric triglyceride molecule populations are triglyceride molecules that consist of a C8:0 fatty acid or a C10:0 fatty acid at the sn-1 position and the sn-2 position, and C16:0 or C18:0 at the sn-3 position. Another population of asymmetric triglyceride molecules are triglyceride molecules that consist of a C16:0 fatty acid or a C18:0 fatty acid at the sn-1 position and the sn-2 position, and C8:0 or C10:0 fatty acid at the sn-3 position. Methods of producing triglyceride oils and using the same are provided using sucrose invertase and hydrogenation of the triglyceride oil. Triglyceride molecules are produced by using recombinant DNA techniques to produce oleaginous recombinant cells.

Agricultural residues (biomasses) have come-up as potential valuable renewable resources for transformation into many bio-products. To achieve this goal, the isolation of major biopolymers in their purest form through an economical process is demanded for synthesis of the target fine chemicals without impurities. However, the difficulty of fractionating the biomass into cellulose, hemicellulose and lignin by a simple method has greatly limited their isolation from agricultural residues. Also, the lipids and waxes present on the surface of the agricultural residues protect it from external chemical and microbial agents. To overcome this issue, the lipids and waxes have been isolated from the biomass through two step organic solvents extraction. Thereafter lignin, hemicellulose and cellulose are isolated successively from the de-waxed biomass.

A process for obtaining free fatty acid and/or free fatty acid ester, including separating a liquid mixture containing the free fatty acid and/or the fatty acid ester by contacting a vapor of the liquid mixture in a column of a distillation apparatus with condensate formed from the vapor running downward. Heat and mass transfer takes place between the vapor and the condensate on column internals. The column has at least 10 theoretical plates and the separation is conducted with a pressure drop between the top and bottom of the column of p of 3.3 mbars. Appropriately, the liquid mixture, contains polyunsaturated fatty acid, preferably omega-6 or omega-3 fatty acid and/or alkyl monoesters and/or glycerol monoesters. In one embodiment the column has at least 30 theoretical plates and the separation is conducted with a pressure drop p between 3.5 mbar and 6 mbar.