The invention provides methods that can be used to separate mixtures of fatty acids and fatty acid esters into individual components or into more concentrated mixtures of individual components.

Methods for obtaining soybean plants that produce seed with low linolenic acid levels and moderately increased oleic levels are disclosed. Also disclosed are methods for producing seed with low linolenic acid levels, moderately increased oleic levels and low saturated fatty acid levels. These methods entail the combination of transgenes that provide moderate oleic acid levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. These methods also entail the combination of transgenes that provide both moderate oleic acid levels and low saturated fat levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. Soybean plants and seeds produced by these methods are also disclosed.

It has been found that esterification of a hydroxy-fatty acid by a lipase can be coupled with oleate hydratase (OHase) generation of that hydroxy-FA from an unsaturated FA with a cis C9-C10 double bond, e.g. oleic acid, in a single aqueous buffered reaction medium at low temperature,e.g. 30 C. A simple one-pot enzymatic method to produce fatty acid estolides from one or more triglycerides, e.g. starting from a natural plant oil, is thereby enabled in which the same lipase catalyses both the initial hydrolysis of triglyceride and the final esterification step.

Systems, methods, and materials for pyrolyzing vegetable oil feedstocks to obtain high yields of various products are described.

A microbial oil comprising dihomo--linolenic acid as a constituent fatty acid of an oil, the microbial oil has a content, in terms of a weight ratio of arachidonic acid relative to dihomo--linolenic acid (arachidonic acid/dihomo--linolenic acid) of less than 1/13. Preferably, the microbial oil has a triglyceride content of greater than or equal to 70% by weight, and a saturated fatty acid content of less than or equal to 40% by weight. Moreover, a lower alcohol ester of dihomo--linolenic acid or a free fatty acid of dihomo--linolenic acid obtained from the microbial oil is provided.

Methods for obtaining soybean plants that produce seed with low linolenic acid levels and moderately increased oleic levels are disclosed. Also disclosed are methods for producing seed with low linolenic acid levels, moderately increased oleic levels and low saturated fatty acid levels. These methods entail the combination of transgenes that provide moderate oleic acid levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. These methods also entail the combination of transgenes that provide both moderate oleic acid levels and low saturated fat levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. Soybean plants and seeds produced by these methods are also disclosed.

The subject invention provides efficient and convenient methods of isolating 9-oxo-10E, 12E-octadecadienoic acid methyl ester (9-KODE methyl ester) from C. versicolor. In addition, the subject invention provides therapeutic uses of Coriolus versicolor extracts, biologically-active chemical substituents isolated from C. versicolor, as well as 9-KODE methyl ester and related compounds. In a preferred embodiment, the subject invention can be used to inhibit the metastatic spread of cancer cells.

A phospholipid composition obtained from the process of forming a homogeneous mixture by mixing (A) a first lecithin extract ingredient with (B) a second lecithin extract ingredient from plants in a predetermined ratio of (1-3):(1-3); and then adjusting moisture of the homogeneous mixture below 10%. In addition, the invention also discloses the phospholipid composition applied to make the nano liquid product containing curcumin have the ability to treat burns and increase the effect of scar healing; wherein the product is used at a dose of 0.05-0.1 mL/cm.sup.2 of skin, with a frequency of twice daily to reduce the area of the burns, and increase the concentration of hydroxyproline in the skin.

The present invention is related to a method for purifying crude -sulfo fatty acid salt obtained by the reaction of fatty acid and sulfur oxides, comprising the following steps: a) diluting the crude -sulfo fatty acid in a solvent and adjusting the ph from 2 to 4; b) heating up the slurry of a-sulfo fatty acid salt obtained in the step a) until being fully solubilized: c) cooling down the solution of -sulfo fatty acid salt obtained in the step b) to room temperature gradually to obtain the crystals; d) filtering the crystal obtained in the step c) to remove the motherinsing the filter cake at least twice with acid aqueous solution to obtain the purified -sulfo fatty acid salt.

A microbial oil comprising dihomo--linolenic acid as a constituent fatty acid of an oil, the microbial oil has a content, in terms of a weight ratio of arachidonic acid relative to dihomo--linolenic acid (arachidonic acid/dihomo--linolenic acid) of less than 1/13. Preferably, the microbial oil has a triglyceride content of greater than or equal to 70% by weight, and a saturated fatty acid content of less than or equal to 40% by weight. Moreover, a lower alcohol ester of dihomo--linolenic acid or a free fatty acid of dihomo--linolenic acid obtained from the microbial oil is provided.