A cell culture apparatus includes a flow passage in which cell suspension containing at least one of cells or cell masses as granular bodies is to flow, and an imaging unit that is provided in a middle of the flow passage and continuously images the plurality of granular bodies contained in the cell suspension to acquire a plurality of images while the cell suspension flows in the flow passage.

Methods and systems are described herein for manufacturing oligosaccharides, including maltosyl-isomaltooligosaccharides. The methods involve removing undesired components from fermentation fluids that contain maltosyl-isomaltooligosaccharides.

Disclosed are devices and methods for non-cryogenic vitrification of biological materials that include the steps of providing one or more capillary channels of which a first opening is operably in contact with a moisture containing vitrification mixture made of a biological material and a vitrification agent.

This cell production device comprises: a cell production plate that includes a fluid circuit in which a plurality of functional sites are integrated; and a closed type connector that connects the fluid circuit to an external space in a closed manner, wherein the fluid circuit comprises, as the plurality of functional sites, an injection and discharge unit that injects or discharges a fluid into or out of the fluid circuit via the closed type connector, a variable volume unit that stores a fluid that is extruded or withdrawn by the injected or discharged fluid, and a cell induction and culture unit that performs at least one of induction and culture of cells based on the injected fluid.

Methods of controlling hydrogen sulfide concentration of a biogas occupying an anaerobic membrane bioreactor (AnMBR) containing a submerged membrane are disclosed herein. Methods of controlling dissolved sulfide concentration of a mixed liquor within the AnMBR are disclosed. The methods include directing wastewater containing sulfur and a chemical oxygen demand (COD) to an AnMBR, withdrawing at least a fraction of the biogas from the AnMBR, directing a pre-determined amount of the withdrawn biogas to a scrubber, directing a remainder of the withdrawn biogas to a gas distributor, and directing the scrubbed biogas to the AnMBR. Systems for treating wastewater having sulfur and COD are disclosed. The systems include an AnMBR, a scouring gas closed loop, a scrubber, and a control mechanism for directing biogas to the scrubber and to a gas distributor. Methods of retrofitting a system for treating wastewater having sulfur and COD are disclosed.

Provided are a bacterium for degrading sludge having a 16S rRNA gene comprising a nucleotide sequence having 97% or more identity to the nucleotide sequence represented by SEQ ID NO: 1, a bacterium having a 16S rRNA gene comprising the nucleotide sequence represented by SEQ ID NO: 1 with mutations of two bases or less, and having an ability to degrade a target microorganism, and a microbial preparation for degrading a target microorganism comprising a bacterium (a1) below. Bacterium (a1) is a bacterium having a 16S rRNA gene comprising a nucleotide sequence having 90% or more identity to the nucleotide sequence represented by SEQ ID NO: 1.

The present disclosure relates to a well plate for 3D cell spheroid culture that facilitates 3D cell spheroid culture and enables cell adhesion and detachment by adjusting a surface roughness, a method for manufacturing the well plate for 3D cell spheroid culture, and a method for 3D cell spheroid culture using the same.

The present invention relates to a decellularized nerve graft using allogeneic and heterologous nervous tissues and a method of manufacturing the same.

In the present invention, by using a low-concentration basic solution and a surfactant as a decellularization solution, cell and tissue toxicity caused by a solvent or surfactant remaining in the tissue may be minimized by minimizing the use of a basic solution and an anionic surfactant in the entire manufacturing process. In addition, a peristaltic pump may be used to maintain the tissue structure and effectively remove lipid and cells.

A lung breathing chip and cell stretching culture platform and an operating method thereof are disclosed. The lung breathing chip and cell stretching culture platform controls the output of the motor by programming, stretches the micro-fluidic chip by the cam component, changes the size of the cam component and the frequency of the motor rotation to change the stretching frequency and the amount of stretching to simulate the breathing of the lungs in different states, uses liquid electrophoresis technology to arrange the cells in the biocompatible hydrogel and the hydrogel three-dimensionally to imitate the three-dimensional cell tissue, and injects drugs through the dynamic perfusion system to realize the drug testing platform that the cells of the chip bionic lung tissue are stretched.

Containment and facility systems for offshore porous floating bioreactors that contain algae water slurries in saltwater environments. A containment system may include a containment boom system, a containment silt curtain system, a containment marineworks system, or a combination thereof. Facility systems include an offshore porous floating bioreactor in fluid communication with at least an onshore algae harvesting system. Algae cultivation may occur at an onshore algae cultivation system or within a porous floating bioreactor, and algae lipid production occurs within a porous floating bioreactor. The lipid induced algae is received by the onshore algae harvesting system for further processing.