The invention concerns a cassette for cell culture comprising: an at least partially rigid housing internally defining an internal space inside which a cell culture bag defining an internal volume is arranged and attached; conduits each connected at one end to the internal volume of the bag and each having a second end situated outside the housing; and valves for enabling/preventing the flow of fluid through the conduits that are mounted on the housing.

A bioreactor assembly component can include a length of tubing having an integrated pump head along its length, with a portion of the tubing extending between the pump head and a container, such as a single-use bioreactor. The integrated pump head can be configured to engage with an external driving component to control the operation of the integrated pump head without breaching the sterility of the integrated pump head. The integrated pump head can include only components which can be gamma sterilized, allowing the bioreactor assembly component to be sterilized and stored before use. The bioreactor assembly component can be used with a control module including a pump driver configured to engage with and control the operation of the integrated pump driver, and can include components which can monitor and control the operation of a bioprocess within a single-use bioreactor.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

In one aspect, a system for use in processing a fluid comprises a reservoir for holding the fluid and one or more bioreactors for receiving the fluid from the reservoir. One or more sensors are provided for sensing one or more parameters of the fluid, and corresponding control measures may be initiated. Regulators may also be provided for regulating various parameters of the fluid processing operation. Related systems and methods are also disclosed.

The present invention provides a continuous upstream manufacturing process for the production of bispecific antibody products, which comprise at least two binding domains. The process comprises at least the steps of (i) providing in a perfusion bioreactor at least one mammalian cell culture, which is capable of expressing the bispecific antibody product, (ii) growing the mammalian cell culture at a first perfusion rate until a set point viable cell density is reached, and (iii) maintaining perfusion culture at a second perfusion rate, wherein the bispecific antibody product concentration in the bioreactor is kept below a threshold value. The bispecific antibody product is then subject to subsequent downstream processing. Moreover, the invention provides a bispecific antibody product produced by the continuous upstream manufacturing process.

A system for growing cells comprising a bioreactor chamber for growing the cells, a delivery system delivering a perfusion solution to the bioreactor chamber for perfusion of the perfusion solution through the cells, a dialysis system having a dialyzer, a dialysate for performing a dialysis and a filter for reducing ammonia content in said dialysate, and a controller that circulates the perfusion solution through the dialyzer and the dialysate through the filter.

The present application describes a large scale process for the in vitro production of an olive cell culture.

The application further describes a composition in a form of a powder comprising olive fruit/leaf cells grown in vitro and a method of treating metabolic syndrome disorders, such as, high cholesterol level, comprising administering an effective amount of the composition. The cell line callus culture of olive cells manufactured according to the process of the invention includes high level of hydroxytyrosoll, tyrosol, oleoropein and verbascoside.

Methods of production and apparatuses for production for stably producing healthy and functional platelets, and methods for determining operating conditions of an apparatus for producing platelets are provided. The present invention relates to methods of production comprising a step of culturing megakaryocytes in a platelet production medium C, wherein the step of culturing comprises a step of stirring a medium C in containers 1, 4 using stirring mechanisms 2, 5. In one method of production, the step of stirring comprises reciprocating stirring blades 21, 24 in the stirring mechanisms 2, 24 such that any of predetermined turbulent energy, shearing stresses, or Kolmogorov scale is produced, in the medium C. In another method of production, the step of stirring comprises pivoting a stirring blade 51 in the stirring mechanism 5 wherein a plurality of stationary members 53 is placed therearound in a bottom region 4e of the container 4 such that the step of stirring produces turbulence in the medium C. The present invention also relates to apparatuses for production for carrying out the method of production. The present invention also relates to a method for determining operating conditions of an apparatus for producing platelets.

A resource treatment system for urine and feces separation and recovery in urine diversion dehydration toilets, includes a urine-faeces division toilet, a urine and gray water treatment system, and a fermentation and biodegradation fecal system. The urine-faeces division toilet is configured to separate and recover urine and feces discharged by users. The urine and gray water treatment system includes an adjusting pool, a microalgae culture device and a metal-based electrogenerated dynamic membrane. The adjusting pool is configured to receive the urine in the urine-faeces division toilet and domestic sewage, and adjust a urine-to-domestic sewage ratio. The metal-based electrogenerated dynamic membrane includes a metal microfiltration membrane, a stainless-steel mesh and a power supply. The fermentation and biodegradation fecal system includes a collection and adjusting device, a fermentation bed and a biodegradation chamber.