Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

A mixed acrylate-siloxane polymer can be used to create three-dimensional (3D) structures of arbitrary shape via nanolithography. Treatment of such structures with amine (such as diamine) makes them permissive for neuronal cell adhesion and growth without need of additional modification such as poly-lysine (D or L) nor laminin.

A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.

Provided is a confined migration microfluidic device for cell culture and drug screening, including a chip including a plurality of parallel channels, where each pair of it is connected through a plurality of the confined migration channels; a depth of the confined migration channel is lower than the first channel and second channel; a first/second inlet and a first/second outlet are provided at two ends of the first/second channel, respectively; and a pyramid-like structure for diverging flow to the first channels to ensure evenly distribution. During the usage, cells to be cultured are added into one of the first channel and the second channel, and drugs or cells which can influence confined migration of the target cells are added into the other channel; the inhibitory effect of the drugs or cells on the confined migration will be evaluated, thereby screening/studying effective drugs and cells in relation to the confined migration.

Described herein is a beads-free bioprocessor as an automated and cost-effective T cell processing and manufacturing platform. T cells are a core component in CAR T cell therapies for cancer treatment, but are difficult to manufacture to scale in clinically relevant quantities. The 3D bioprocessor provides an alternative device that is scalable, beads-free, easy-to-use, and cost-effective for using CAR T cell therapy in cancer immunotherapy. Besides CAR T cell application, this platform technology has potential for many other applications such as cancer cell isolation.

A sensor device according to the present technology includes a stack sensor. The stack sensor includes a first sensor layer and a second sensor layer. The first sensor layer has, as a detection target, a first substrate in a culture solution, the first substrate being changed in accordance with a change in a state of a cell. The second sensor layer has, as a detection target, a second substrate in the culture solution and is provided on the first sensor layer, the second substrate being changed in accordance with the change in the state.

A mixed acrylate-siloxane polymer can be used to create three-dimensional (3D) structures of arbitrary shape via nanolithography. Treatment of such structures with amine (such as diamine) makes them permissive for neuronal cell adhesion and growth without need of additional modification such as poly-lysine (D or L) nor laminin.

A device for growing microorganisms. The device includes a body member comprising a self-supporting, water-proof substrate having upper and lower surfaces; a hydrophobic spacer element adhered to the upper surface of the substrate forming side walls to retain a predetermined amount of liquid in contact with the substrate, wherein the hydrophobic spacer element has a hole therein; a fluid control film in the hole of the hydrophobic spacer element; a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member; and a substantially dry, first microbial growth nutrient composition disposed on a portion of the inner surface of the cover sheet; a first adhesive composition adhered to the first microbial growth nutrient composition; and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition.

A cell culture apparatus having a cell-holding container and a pinholder-shaped member comprising needle-shaped bodies arranged on a substrate, wherein a protruding part is formed in the center of the bottom surface of the cell-holding container, a recessed part is formed between the center and a side wall, and through-holes through which the needle-shaped bodies penetrate are established on the bottom surface of the recessed part; the needle-shaped bodies are arranged in correspondence with the positions of the through-holes; and the pinholder-shaped member is arranged, such that a tip-side portion of each of the needle-shaped bodies penetrates through the corresponding through-hole from the bottom surface side or upper surface side of the cell-holding container.

A cell culture kit is provided. The cell culture kit allows a cultured cell to be easily separated when the cell is cultured and separates a cell without separate trypsinization for subculture.