The present disclosure relates to bioreactor tiles including fluidic channels and optical waveguides. One example system includes a substrate having a first channel, a second channel, and a third channel defined therein. The three channels are separated from one another by partial wall structures. The system also includes an optical waveguide configured to receive illumination light at a first end of the optical waveguide; propagate the illumination light toward a second end of the optical waveguide; allow at least a portion of the illumination light to escape the optical waveguide from a first surface of the optical waveguide as the illumination light propagates toward the second end of the optical waveguide; and provide the portion of the illumination light that escapes the optical waveguide from the first surface to the second channel or the third channel.

The disclosure provides for multilayered organ-on-a-chip systems that can be used to generate topographic neural organoids, and uses thereof, including as models to study neurological disorders.

A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm.sup.2.

A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm.sup.2.

The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated.

A syntrophic enrichment for enhanced digestion (SEED) system is presented, in which a retrofit addition to existing anaerobic digestion infrastructure provides improved digestion process rate and biogas quality. The system provides optimal niche environments for accelerating fermentative, syntrophic and methanogenic metabolisms to increase digestion system loading rates and enhance main digester microbiome. Prescribed media formulations, reactor integrations, and operational methods using various fixed and loose media enhance global digestion system performance. The retrofitted system enables existing plants to transition from an outdated solids-management model to one of valorized biomethane production.

A cell culture instrument configured to detach cells at a desired position and a cell processing method using the cell culture instrument. The cell culture instrument includes: a substrate; and a photoreactive layer having a photosolubility and a photothermal convertibility, wherein the photoreactive layer is laminated on the substrate, and the photoreactive layer includes a polymer having a photosolubility and a photothermal convertibility.

The disclosure relates to bioreactors, for example for biological treatment and, more specifically to bioreactor insert apparatus including biofilms and related methods. The bioreactor insert apparatus provides a means for circulation of reaction medium within the bioreactor, a biofilm support, and biological treatment of an inlet feed to the reactor/insert apparatus. The bioreactor insert apparatus has a high relative surface area for biofilm attachment and is capable of generating complex flow patterns and increasing treatment efficiency/biological conversion activity in a biologically-active reactor. The high surface area structure incorporates multiple biofilm support structures such as meshes at inlet and outlet portions of the structure. The biofilm support structures and biofilms thereon can increase overall reaction rate of the bioreactor and/or perform some solid/liquid separation in the treatment of the wastewater or other influent.

Apparatus and methods for monitoring embryos in an incubator are described. The apparatus comprises an incubation chamber defined by an incubation chamber housing and a sample platform comprising a plurality of receptacles for holding embryos within the incubation chamber for incubation. The sample platform may comprise a slide carrier on which embryo slides containing the receptacles may be placed incubation. The sample platform is rotatable about a rotation axis relative to the incubation chamber housing so as to allow receptacles to be selectively rotated to a monitoring position that is aligned with a viewing port provided in the incubation chamber housing, for example an opening or window. An imaging device is located outside the incubation chamber and arranged to image embryos within the incubation chamber at the monitoring position through the viewing port.

An impedance spectroscopy biosensor is provided that is fabricated on a stretchable substrate. The stretchable substrate is integrated with an impedance biosensor that undergoes cyclic strain without cracking. The biosensor is formed by curing an elastomer precursor while on a pre-tensioned membrane that includes a conductive electrode. The resulting elastomeric material is released from the support after curing which releases the pre-tensioned state to produce the biosensor.