An automated method includes providing a dry ingredient to be reconstituted into a liquid bioprocess solution and controlling, by a processing circuit, all automated system including at least one mixing chamber, an array of tubing for fluid flow within the system, and a plurality of valves provided within the tubing, to automatically prepare the liquid bioprocess solution from the dry ingredient. Controlling the auto system may include performing a series of sequential mixing steps, the series of sequential mixing steps causing the preparation of the liquid bioprocess solution. The method mixing include taking one or more measurements during the preparation of the liquid bioprocess solution, wherein each step is triggered by at least one of a measurement decreasing below, equaling, or exceeding a measurement threshold. Each step may also include opening or closing, by the processing circuit, at least one of the plurality of valves.

A method for mixing a fluid includes: dispensing a first volume of a fluid into a flexible container, the flexible container being at least partially disposed within the chamber of a support housing; repeatedly moving the support housing and the flexible container contained therein so as to mix the first volume of fluid within the flexible container; adding further fluid into the flexible container after moving the support housing to form a second volume of fluid; and manipulating a mixing element within the flexible container so as to mix the second volume of fluid.

An arrangement for and a method of biomass hydrolysis includes a feed pump, a pre-hydrolysis reactor and a feed line there-between for feeding fresh biomass slurry to the pre-hydrolysis reactor. The feed pump is a centrifugal feed pump and the pre-hydrolysis reactor is an upflow reactor.

A cell culture device includes a storage tank having one or a plurality of cell culture units. The cell culture units includes a culture liquid main chamber having a circulation space through which a culture liquid of cells circulates, a permeable membrane having one surface to which the cells are able to adhere, said one face facing the circulation space, a first culture liquid storage chamber having an airtight structure in which the culture liquid is to be stored, a second culture liquid storage chamber in which the culture liquid is to be stored, a culture liquid introduction flow path that introduces the culture liquid from the first culture liquid storage chamber into the circulation space of the culture liquid main chamber, and a culture liquid lead-out flow path that introduces the culture liquid from the circulation space into the second culture liquid storage chamber.

The microorganism preparation feeding method of the invention employs an automatic microorganism preparation feeding apparatus which includes a cold storage apparatus for refrigeration-storing a seed microorganism belonging to the aerobic microorganism group including at least one species of aerobic microorganisms capable of decomposing oil and fat contained in oil/fat-including wastewater and a growth tank for growing the seed microorganism so as to produce the microorganism preparation, wherein the seed microorganism belonging to the aerobic microorganism group is maintained in a live state by means of the cold storage apparatus, the seed microorganism is periodically grown by means of the growth tank so as to produce a predetermined microorganism preparation, and the produced predetermined microorganism preparation is fed to the oil/fat-including wastewater. The method includes refrigeration-storing, as the seed microorganism, a microorganism whose population density is 1×10.sup.7 CFU/mL to 5×10.sup.9 CFU/mL in the cold storage apparatus; growing, as a source material, the seed microorganism of a predetermined volume by means of the growth tank so as to produce the predetermined microorganism preparation whose volume is 50 to 500 times the predetermined volume of the seed microorganism and whose population density is 1×10.sup.7 CFU/mL to 2×10.sup.10 CFU/mL; and feeding the produced microorganism preparation to the oil/fat-including wastewater.

An apparatus for culturing cells includes a bio reactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed beds arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

The embodiments herein disclose a method and apparatus for mixing sludge retained in a digester. A jet nozzle assembly for mixing contents of a vessel is used. The jet nozzle assembly having a central outlet pipe terminating at a jet nozzle, a low pressure nozzle assembly disposed concentrically about the central outlet pipe, the low pressure low pressure nozzle having a plurality of openings disposed circumferentially about the low pressure nozzle assembly. The plurality of openings are an axial distance from the outlet jet nozzle.

The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.

The present invention provides for a fully integrated microfluidic system capable of producing single-dose amounts of biotherapeutics at the point-of-care wherein protein production, purification and product harvest are all integrated as a single microfluidic device which is portable and capable of continuous-flow production of biotherapeutics at the microscale using a cell-free reaction system.

The insert shelf (1) is characterized in that it has at least one means (3) with which an incubation medium located in an incubation vessel placed on the insert shelf (1) can be set in motion during an incubation treatment or during an incubation process in an incubator (2). For this purpose, the at least one means (3) can be operated electrically and/or preferably moved mechanically.