A photobioreactor system and a process for its use is illustrated, whereby water and nutrients from multiple sources are balanced (mixed using aeration) to the specific requirements of the particular photosynthetic organism strain used, sterilized, further mixed to balance the system and seeded with the photosynthetic microorganism, e.g. microalgae (dilution of a concentrated stock or added to an existing algal biomass). In accordance with such an embodiment, the algal biomass is then grown for a most efficient number of hours in a totally controlled environment where temperature (using aeration, an internal coil cooling system, or a combination thereof), pH (via CO.sub.2 delivery) and light delivery (using internal lighting directly inside the algal biomass) are optimized to the algal strain grown.

A container (1) for in vitro diagnostic has a lower receptacle (2) designed to contain a non-toxic liquid (LS), an upper receptacle (3) designed to contain a toxic liquid (LF), and a connecting sleeve (4), in a threaded coupling with the lower receptacle (2) and the upper receptacle (3) for butt joining them. The connecting sleeve (4) has a transversal septum (40) being provided with a plurality of passage openings (48) and vent openings (49). When the container (1) is completely closed, both the lower receptacle (2) and the upper receptacle (3) abut the transversal septum (40). If the upper receptacle (3) is partially unscrewed with respect to the transversal septum (40), the toxic liquid (LF) flows by gravity into the lower receptacle (2). A method for storing a sample of human or animal tissue by using the container (1) is also provided.

A cell culture device comprises a top layer, a diffusion layer, and a suspension layer. The diffusion layer comprises at least one diffusion channel. The suspension layer comprises multiple suspension units. The multiple suspension units are connected to the diffusion channel. A fluid is conducted through the diffusion channel and flow into the suspension units for drop suspended. Cells are cultivated in the fluid suspended in the suspension unit. The cell culture device can be used in an electromagnetic cell culturing system. The electromagnetic cell culturing system is a combination of hanging drop method, microfluidic technology and electromagnetic stimulation. The electromagnetic cell culturing system can improve the growth of cells and also is capable of continuously providing culture medium into the cell culture device. The cell culture device can be used to cultivate large quantity of cells with high productivity and stable quality.

The present disclosure provides improved methods and systems for transfecting host cells with nucleic acids, such as plasmid DNA, for purposes of efficiently producing biological products, such as AAV vectors, at large scale.

A method displacing a fluid and simultaneously gas enriching a liquid cell culture medium with a gas. The method includes injecting a controlled volume of a gas or gas mixture into a one chamber by using a gas flow controller, the injection taking place through a gas inlet into a volume of liquid. This injection produces bubbling and agitation of the volume of liquid; a build-up of gas or gas mixture due to buoyancy in a hermetic space formed by the volume of liquid and the chamber, and a pressure increase in the chamber until a sufficient controlled pressure is reached of less than or equal to 10 bar. This increase displaces the volume of liquid by a fluid outlet connecting the volume of liquid to the exterior of the chamber. Also provided are a device implementing the method and a cell culture system in a multi-well culture plate.

A bioreactor for culturing of cells is described. Screens suitable as a cell growth scaffold may comprise crossed fibers. Screens may be contained loosely in a screen holder, which in turn may be contained inside a manifold assembly. A lower manifold, screen holder and upper manifold may have identical or similar interior open cross-sections. Flow of liquid medium can occur upwardly through the array of screens, then flowing over a weir in the presence of an air pocket, and into a moat and a pump. The screen holder may have slots whose exterior-facing ligaments are rounded, and may have grooves whose interior-facing edges are rounded. These components may be located inside an incubator suitable to maintain desired environmental conditions and cleanliness.

A cell culture apparatus includes: a supply unit that supplies a culture medium to a culture tank accommodating the culture medium and cells; a discharge unit that discharges the culture medium and the cells from the culture tank; and one or more processors comprising hardware, the one or more processors being configured to determine whether or not cell density in the culture medium in the culture tank has exceeded a preliminarily set predetermined threshold, wherein in response to determining that the cell density has exceeded the predetermined threshold, the one or more processors are configured to cause the discharge unit to discharge a portion of the culture medium and the cells from the culture tank and cause the supply unit to supply a new culture medium to the culture tank to reduce the cell density to the predetermined threshold or less.

A bioreactor is provided herein. The bioreactor includes a vessel having a wall at least partially defining an interior compartment for receiving fluid, at least one port, and at least one cap configured to removably engage with the at least one port, the at least one cap comprising a filter material. A cell culture method is also provided herein which includes adding cells and cell growth medium to a vessel of a bioreactor and adding microcarriers to the vessel to form substantially confluent cells on the microcarriers. The cell culture method further includes washing the confluent cells, harvesting the confluent cells to form a solution containing the cells, and removing the solution containing the cells from the vessel by flowing the solution through a filter material in a cap removably engaged with at least one port of the bioreactor.

Disclosed mechanical stimulator systems can maintain the sterility of biological samples within a multi-well plate while mechanically stimulating the samples. The systems disclosed herein allow for the individual activation by mechanical compression of a high number of samples at the same time, while maintaining sterility of the samples. The well plates, guides, actuations pistons, and other components of the disclosed systems can be configured to be contained in or adjacent to an incubator that can control properties such as gas levels and temperature while at the same time allowing for the mechanical actuation of the biological samples.

Photobioreactor devices and units for the production of biomass and remediation of environmental contamination are provided. The bioreactor devices comprise (i) a photobioreactor unit comprising a first membrane layer and a second membrane layer, the two membrane layers arranged such that at least a portion of the first membrane layer is directly bonded to at least a portion of the second membrane layer in order to form a defined boundary around non-bonded portions of the first and second membrane layers, thereby defining the photobioreactor unit capable of containing a fluid, wherein at least one of the first and second layers is translucent, and wherein at least a part of the first and second membrane layers is permeable to gases. The permeability coefficient of oxygen through the first and/or second membrane layer is suitably not less than about 100 Barrer, typically not less than about 300 Barrer, and suitably not less than about 500 Barrer. The photobioreactor unit also comprises an inlet and an outlet port to enable the fluid to circulate through said unit. Systems comprising the devices are provided as well as methods of using the devices for the production of biomass, remediation of wastewater and removal of atmospheric pollutants.