A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

The process comprises the following steps: collecting a determined volume in a sample (4), likely to contain at least one microorganism (2), splitting the collected volume into a plurality of compartments (6), the volume of each compartment (6) being smaller than 10 L, incubating at least one microorganism (2) in the compartments (6), detecting compartments (6) comprising at least one microorganism (2), extending the incubation until detecting a defined quantity of microorganism (2), recovering the content of the detected compartments (6) in receptacles (82) for subsequent use, determining at least one functional parameter relative to a microorganism (2) in the detected compartments (6, 6), the steps for splitting, incubation, detection and determining at least one functional parameter being integrated into an automated system (1) for isolating and analyzing microorganisms (2).

A two-vessel continuous flow system in conjunction with low mutation reduced genome bacterial strains provides a platform for long term extended fermentations. Such systems require modification of standard fermentation devices such as probes, pumps and monitoring systems as well as improved procedures for feed delivery, culture monitoring and product harvesting methods. An optimized two-vessel system for producing large quantities of fermentation products from small volume, long duration continuous fermentations represents a significant improvement over existing fermentation strategies. Methods and compositions for long term continuous flow fermentation using a two vessel continuous culture fermentation apparatus are described.

A homogenized and integrated device with a coaxial line and double-high pressure cylinder, includes a long oil cylinder, two main connecting sleeves, two high pressure cylindrical homogenized main bodies, two auxiliary connecting sleeves and two short oil cylinders. The two main connecting sleeves, two high pressure cylindrical homogenized main bodies, two auxiliary connecting sleeves and two short oil cylinders are respectively and symmetrically arranged at two ends of the long oil cylinder and are assembled with the long oil cylinder along a same axial line. Each high pressure cylindrical homogenized main body is integrally connected with the long oil cylinder by virtue of one of the main connecting sleeves. Each high pressure cylindrical homogenized main body is integrally connected with the corresponding short oil cylinder by virtue of one of the auxiliary connecting sleeves.

Embodiments are described that relate to methods and systems for growing cells in a hollow fiber bioreactor. In embodiments, the cells may be exposed to an activator for activating expansion of the cells. The cells may in embodiments include T cells, and the activator may be in different forms, including, for example, antigen presenting cells or beads functionalized with antibodies.

An apparatus (100) for mixing multiphase flowing particles. The apparatus (100) comprises a conduit (100a) adapted to channelize the multiphase flowing particles. At least one flow diverter (101) is positioned in the conduit (100a), which is adapted to divert the flow of multiphase flowing particles into a plurality of flow streams. Further, at least one flow element (102) is disposed in the conduit (100a) along at least one of the plurality of flow streams, which is configured to inject fluid onto the plurality of flow streams at a velocity greater than the velocity of the plurality of flow streams. This induces a swirling flow of at least one of the plurality of flow streams, thereby facilitating mixing of the multiphase flowing particles in the conduit (100a).

There are provided a device and a method for more appropriate evaluation of a chemical substance. The device for the evaluation of the chemical substance includes a first compartment, and a second compartment that communicates with the first compartment and is separated from the first compartment by a partition wall.

In a method for producing a cell concentrate using a cell suspension treatment system including a storage container of a cell suspension, which has a solution inlet port, a circulation outlet port, and a circulation inlet port, a cell suspension treatment device for concentrating the cell suspension by separating liquid from the cell suspension by filtration, the device including a container having a cell suspension introduction port, a cell suspension lead-out port, and a filtrate outlet, which is filled with a hollow fiber separation membrane, a circulation circuit for concentrating the cell suspension while circulating the cell suspension between the storage container and the cell suspension treatment device, a collection container of a cell concentrate obtained by concentration, a collection path for feeding the cell concentrate to the collection container, an injection path for injecting a solution into the solution inlet port of the storage container, and a detecting unit.

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.

A system for performing a gas-liquid mass transfer includes a container bounding a compartment and having a top wall, a bottom wall, and an encircling sidewall extending therebetween. A tube has a first end and an opposing second end, the first end of the tube being disposed within the compartment of the container. A nozzle is disposed within the compartment of the container and has at least one outlet, the nozzle being coupled with the tube so that a gas can be passed through the tube and out the at least one outlet of the nozzle. The nozzle is sufficiently buoyant so that when a fluid is disposed within the compartment of the container, the nozzle floats on the fluid.