Provided are a cell culture apparatus and a living cell concentration adjustment method. The cell culture apparatus includes: a first container that holds a cell suspension; a second container that holds a diluent diluting the cell suspension; a living cell concentration measurement device that individually determines whether a plurality of target cells in the cell suspension are alive or dead to measure a living cell concentration of the target cells in the cell suspension; a control device that holds and controls the living cell concentration and amount data of the cell suspension; a mixing unit that mixes the cell suspension and the diluent; and a culture container in which the diluted cell suspension is seeded to culture cells. The control device determines an amount of the cell suspension to be seeded and an amount of the diluent required to adjust the cell suspension to be seeded to a predetermined living cell concentration, on the basis of the living cell concentration and the amount data of the cell suspension, feeds the amount of the cell suspension and the amount of the diluent to the mixing unit, and seeds the diluted cell suspension.

An incubation system for gas detection of a biological specimen can include a gas or optical sensor. The sensor can be arranged to be placed in communication with at least one biological specimen vessel for generating an electrical response signal indicating a chemical characteristic associated with the specimen. The specimen can be incubated in a temperature-controlled chamber, such as within a shelf defining a plurality of receptacles. Processing circuitry can be included or used such as to place the sensor and at least one biological specimen vessel in communication with each other.

A system for aerobic fermentation includes a vessel, an aeration system including a gas sparger fluidly coupled to the vessel to introduce a compressed gas to an internal volume of the vessel, and a recirculation loop fluidly coupled to an outlet of the vessel. The recirculation loop includes an eductor fluidly coupled to an oxygen-containing gas source, a static mixer downstream of the eductor, a heat exchanger downstream of the eductor, and a distributor downstream of the heat exchanger. The distributor is fluidly coupled to the vessel. The aeration system provides mixing and oxygen mass transfer to the fermentation composition in the vessel. The fermentation composition passes through the eductor, static mixer, heat exchanger, and distributor of the recirculation loop, and back into the vessel. Oxygen is transferred from an oxygen containing gas to the fermentation composition and heat is removed from the fermentation composition in the recirculation loop.

An automated cell culture system includes a cell culture reactor including a housing; a fluidic circuit for cell culture media, the fluidic circuit disposed in an interior of the housing. The fluidic circuit includes a culture vessel for culturing cells in the cell culture media, a reservoir for the cell culture media, the reservoir fluidically connected to the culture vessel, and a pump configured to pump the cell culture media in the fluidic circuit. The automated cell culture system includes one or more sensors disposed in the interior of the housing, each sensor configured to detect a parameter of one or more of (1) the cell culture media in the fluidic circuit and (2) an environment in the interior of the housing; and a computing device configured to automatically control operation of the cell culture reactor based on one or more of the detected parameters.

The embodiments herein disclose a method and apparatus for mixing sludge retained in a digester. A jet nozzle assembly for mixing contents of a vessel is used. The jet nozzle assembly having a central outlet pipe terminating at a jet nozzle, a low pressure nozzle assembly disposed concentrically about the central outlet pipe, the low pressure low pressure nozzle having a plurality of openings disposed circumferentially about the low pressure nozzle assembly. The plurality of openings are an axial distance from the outlet jet nozzle.

A cell mass dissociator for a pluripotent stem cell production system.

Organ-on-chip platforms with reduced fluid volumes include circulating fluid volumes of below 1000 L, preferably about 500 L or less. The platforms are adjustable for culturing cells with varied oxygen demand at various seeding densities. The platforms include at least one lane, wherein each lane includes at least one cell culture well, at least one oxygenator for fluid oxygenation, and a pump system containing at least one pump per lane. The oxygenator may include a separate fluid path for oxygenating the fluid, which allows controlling and measuring the oxygen concentration in the fluid, shortening the diffusion length, and passively diffusing oxygen. Provided are also different configurations for the oxygenator, fluid circulation in the platforms, attachment means for securing scaffolds to culture wells, and pneumatic plates.

The present invention relates to an anaerobic column reactor for biodegradation of wastes. Particularly the present invention relates to a process for conversion of biodegradable wastes to biogas and compost. More particularly, the present invention relates to an anaerobic reactor with unique arrangement of expanded and constricted portions alternatively placed vertically over each other which enhances the mixing pattern and thereby the mass transfer rates while controlling the biomass washout by regulating the upflow liquid velocity.

A culture container is provided that has an in vivo-like environment for culturing cells or embryos. The culture container includes rooms for individually receiving the cells or the embryos, wherein two or more rooms are connected via a space allowing passage of culture solution but preventing passage of cells or embryos; and a culture dish including the culture container. The culture dish is provided for supplying nutrient components and so forth as well as removing and discharging waste products. The culture dish includes a reserve tank and pathway for stably feeding fresh culture solution, and a waste tank.

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.